Posted by admin on Dec 25, 2014 in |
The aim of the present study was to investigate the effects of Eclipta alba leaves extract on the biochemical parameters in alloxan induced diabetic Swiss albino mice. Diabetes was induced in mice by injecting intraperitoneally alloxan monohydrate at dose of 135 mg/kg body weight. Ethanolic extracts of E. alba leaves at dose of 200 mg/kg body weight were given orally in diabetic mice daily for four weeks after established LD50 value. Our studies was extended to include the effect of the tested doses on different biochemical parameters including serum glucose concentration, transaminases and phasphatse activities, total protein, albumin, urea, uric acid, and creatinine levels in serum. In diabetic mice, serum glucose concentration, transaminases and phasphatse activities, urea, uric acid and creatinine levels were significantly increased but level of serum total protein and albumin were decreased in comparison with the control group. Diabetic mice group, treated with ethanolic extract of E. alba leaves (200 mg/kg b. wt.), on comparison with diabetic group showed a significant decrease in transaminases and phasphatse...
Read More
Posted by admin on Dec 25, 2014 in |
The objective of study to evaluate a drug Diazepam applied transcranially to brain targeted drug delivery to screening for its amnesic effects, in different learning and memory paradigms viz., rod walking test, locomotor activity test, elevated plus maze test, water maze test, pole climbing test and pole climbing test on trained animal. Diazepam 4mg/ kg intraperitoneal used as a standard for comparing the test drug diazepam 4mg/ kg applied transcranially, whereas both water for injection intraperitoneally and seasme oil transcranially used as a control group. In rod walking test and locomotor activity test used mice for evaluating transfer latency and mean locomotion for 05 minutes and it was observed that Diazepam transcranially has a significant amnesic effect (p<0.0001) as compared to control, and as same like standard. In elevated plus maze, morris water maze, and the pole climbing test have been used rats for evaluating transfer latency, escape latency and avoidance latency; it was observed that Diazepam transcranial has a significant amnesic effect (p<0.0001) as compared to control, and...
Read More
Posted by admin on Dec 25, 2014 in |
Members of the genus Ipomoea of family Convolvulaceae besides being some of the most beautiful ornamental plants cultivated for their bell shaped flowers, are also of medicinal value, with some of them reported as cytotoxic agents against some cancer cell lines. The present study evaluates the anticancer activity of the leaf extracts of ten Ipomoea Jacq. Species growing in and around Mumbai, India, on the A 549 cellline and Hep-G2 cell line evaluated by the SRB assay. The crude aqueous extracts of leaves of Ipomoea species studied had significant cytotoxic effect on HEP G2 Cell Line and showed significant inhibitory effect on A549 cell line. , I. Cairica was the best growth inhibitor (GI50at 30.6µg/ml) for HEP G2 cell line followed by I. Hederifolia (GI50 at 38.1 µg/ml), I. Carnea (GI50 at 40.0 µg/ml). Considering the A549 cell line, none of the crude extracts showed GI50 inhibition upto80 µg/ml with the exception of I. aquatic which showed GI50at 75.6...
Read More
Posted by admin on Dec 25, 2014 in |
Objective: Pseudomonas aeruginosa has emerged as an important cause of serious wound infection and death in burn patients. There are a limited numbers of antimicrobial agents affecting the bacterium due to its inherent resistance. Kefir is a natural probiotic which contains diverse spectrum of bacteria, yeasts and their metabolites with potential antimicrobial activity. Current study was designed to investigate the antimicrobial activity of Kefir extract against P. aeruginosa (ATCC 27853) as well as burn clinical isolates. Materials and Methods: In this descriptive study, the Kefir fermented in MRS Broth medium for 24 hours (hrs), 48 hrs and 96 hrs and their extract were used for evaluation of antibacterial effect. The antibacterial effect of different kefir extract were compared based on measurement of diameter of inhibition zone in diffusion plate method, and assessment of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The density of bacteria and percentage of organic acids (lactic and acetic acids) were also tested. Kefir gels were prepared by mixing of equal portions of gel...
Read More
Posted by admin on Dec 25, 2014 in |
The rates of oxidation of uracil and chlorogenic acid (CGA) by tert-butoxyl radicals have been studied by measuring the absorbance of uracil at 258 nm and CGA at 328 nm spectrophotometrically. tert-butoxyl radicals are generated by the photolysis of tert-butyl hydroperoxide in presence of tert-butyl alcohol to scavenge ·OH radicals. The rates and the quantum yields (f) of oxidation of CGA by t-BuO·radicals have been determined in the absence and presence of varying concentrations of uracil. An increase in the concentration of uracil has been found to decrease the rate of oxidation of CGA suggesting that uracil and CGA compete for t-BuO·radicals. Using competitive kinetic studies, the rate constant for the reaction of t-BuO· radical with uracil has been found to be 4.43´ 108 dm3 mol-1 s-1. The quantum yields (fexpt) have been calculated from the experimentally determined rates of oxidation of CGA under different experimental conditions. Assuming that CGA acts as a scavenger of t-BuO·radicals only, the quantum yields (fcal) have been theoretically calculated. fexpt and fcal values...
Read More
