Posted by admin on Nov 1, 2014 in |
Accurate and sensitive assay methods for quantitative determination of Rutoside trihydrate (rutin), Trypsin and Bromelain in tablet formulation based on UV-spectrophometric analysis were developed and validated. This three drug combination has anti-inflammatory properties and hence is used for treatment of edema and inflammations. Rutin was analyzed directly by UV-Vis spectrophotometer at 257nm because Trypsin and Bromelain are not UV-active therefore they don’t interfere in the analysis of rutin. Trypsin and Bromelain being proteolytic enzymes, Casein was used as substrates for their analysis. Casein was standardized according to Bradford assay using Bovine serum albumin as a standard. Casein was hydrolyzed to amino acid tyrosine by both the proteolytic enzymes. Tyrosine formed was further reacted with Folin-Ciolcalteu (F-C) reagent and absorbance was recorded at 785nm. Trypsin was inhibited by specific trypsin inhibitor Nα-p-tosyl-L-lysine chloromethyl Ketone (TLCK) and specific proteolytic activity of Bromelain was analysed using casein. Bromelain was inactive towards N-a-benzoyl-DL-arginine p-nitroaniline (BApNA) therefore; BApNA was used as a specific substrate for analysis of Trypsin. BApNA was hydrolyzed to pNA by...
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Posted by admin on Nov 1, 2014 in |
The pH-sensitive microcapsules were prepared by interfacial polymerization, in which caffeine was used as a model drug and hyperbranched poly L-lysine (HBPL) and terephthaloyl chloride as wall-forming materials. The microcapsules were characterized by Malvern particle size analysis, scanning electron microscopy, Fourier transforms infrared spectrophotometry (FTIR) and UV-vis spectrophotometer. Triton X-100 was used as the emulsifier of interfacial polymerization. The effects of the emulsifier content on the particle size distribution, loading content, encapsulation efficiency and drug release property of microcapsules were discussed in detail. The release behaviors of caffeine in different phosphate-buffered saline (PBS) pH were investigated systematically. The FTIR analysis of caffeine-containing microcapsules demonstrated that caffeine was successfully encapsulated in the wall-forming materials. The resultant microcapsules had narrower particle size distribution, smoother surface, and higher drug release amount with the percentage weight of emulsifier being 1.5 wt%. The drug-release behavior of caffeine from microcapsules was evaluated as a function of pH. More than 87.1% caffeine was released into pH 7.6 PBS after 100 min, whereas only 37.0% caffeine was...
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Posted by admin on Nov 1, 2014 in |
The uses of plant medicines are very common in Asia and developing countries. Phytochemicals has been used to treat chronic as well as infectious diseases. Cardiospermum halicacabum Linn. (Sapindaceae) is an herbaceous climber, commonly found throughout India, used as vegetable as well as traditional medicine for rheumatism, lumbago and nervous diseases. Many pharmacological studies have been done with various parts of C. halicacabum plant to demonstrate its medicinal effect. The present work was aimed to isolate the active compounds from the leaf extract of C. halicacabum. The hydro alcoholic leaf extract (80% methanol + 20% distilled water) was subjected to isolation procedures for identification of active compounds. Eleven compounds were got and in vitro anti oxidant analysis were done with the isolated compounds.Compound 1 and 8 selected for structural elucidation. The NMR spectrum showed characteristic peaks of compound 1 and 8 and the active phytoconstituents were identified as Kaempferol- 3- o- α- l- rhamnoside and Apigenin – 7- o- β – d- glucuronide by structural elucidation. The isolated compounds...
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Posted by admin on Nov 1, 2014 in |
Antimicrobial effects of different extracts of lichens were evaluated in this study. Acetone extract of Heterodermia boryi have shown maximum inhibitory activity against Escherichia coli (22mm) followed by Staphylococcus aureus (21mm)and Pseudomonas fluorescenes (20 mm). Among fungi, diethyl ether extract gave desirable sensitivity pattern against Pestalotia foedans was very sensitive (21mm) followed by Paeciliomyces variotii (20mm). Most of the extracts of Sticta weigelii were found to be inactive against Fusarium oxysporum. Klebsiella pneumoniae was found to most resistant bacteria against Sticta weigelii. Among the tested microbial strains, Pseudomonas fluorescens was mostly inhibited (26 mm) by chloroform extract of Dermatocarpon vellereum. Serratia marcescens was found to be the most resistant strain against the lichen, but was effectively inhibited only by ethanolic extract of Dermatocarpon vellereum (20 mm). Hexane extract D. vellereum have shown maximum inhibitory activity against Staphylococcus aureus (23...
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Posted by admin on Nov 1, 2014 in |
A simple, rapid and precise stability indicating reverse phase high performance liquid chromatographic method has been developed for simultaneous estimation of Miglitol and Metformine Hydrochloride in their tablet dosage form. Chromatography was performed on a Phenomenex ODS C18 (250 X 4.6 mm) 5mm column using Water: Methanol (50:50, v/v) pH: 4 mixture as a mobile phase. The detection was carried out at 235 nm with a flow rate of 1.0 mL/min. The retention times were 4.807 and 3.273 minutes for Miglitol and Metformine Hydrochloride, respectively. The linearity of the method was excellent over a concentration range 2.5 to 7.5μg/mL for Miglitol and 25 to 75μg/mL for Metformine Hydrochloride. The correlation coefficient was 0.997 and 0.999 for Miglitol and Metformine Hydrochloride, respectively. The limit of detection was 0.6607μg/mL and 1.740μg/mL for Miglitol and Metformine Hydrochloride, respectively. The limit of quantitation was 2.0021μg/mL and 5.2736μg/mL for Miglitol and Metformine Hydrochloride, respectively. The relative standard deviation values for repeatability, intraday precision and interday precision studies were less than 2 % and %...
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