Posted by admin on Nov 1, 2014 in |
To synthesize structurally modified novel Glycogen Synthase Kinase-3βinhibitors to overcome insulin resistance in type 2 diabetes mellitus and to overcome associated health problems with reducing morbidity, mortality and economic costs of diabetes. Methods. Nineteen Phenylmethylene hydantoin analogs were designed and docked in the ATP binding site of GSK-3β by Molegro Virtual Docker 2006.1.5. The synthesis of proposed six hydantoin analogs were performed based on docking results by two approaches, Knoevenagel condensationand Steglich Esterification reaction, keeping the hydantoin ring and different ester substitution at benzylidene ring system can afford potent and selective GSK-3β inhibitors involved in the control of glycogen metabolism. The antidiabetic activity and liver glycogen content were also determined against Streptozotocin induced diabetic rat model. Results. The synthesized compounds H-a, H-c and H-e increased hepatic glycogen content in range 410-420 mg/gm liver weight while compounds H-b, H-d and H-f cause increased in hepatic glycogen content in range of 357-370 mg/gm liver weight as compared to control having 242.01 mg/gm liver weight. Conclusion. The synthesized compounds decreased blood glucose...
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Posted by admin on Nov 1, 2014 in |
The inhibitory effect of Eucalyptus globulus oil was evaluated against the E. coli and the minimum inhibitory concentration (MIC) value of Eucalyptus globulus oil was calculated by agar dilution assay, which was found to be 1µl/ml. The E. coli biofilm was formed on catheter surface and quantified by crystal violet staining system. The crystal violet quantification method showed maximum biofilm after 48h. The effect of Eucalyptus globulus oil was evaluated against E. coli biofilm and was found to inhibit the biofilm by 62%. To evaluate the effect of oil containing silica nanoparticle against biofilm system, the E. globulus oil was loaded in the silica nanoparticle. The synthesized nanoparticle (SNP) was characterized using scanning electron microscopy (SEM), and data showed the average size of silica nanoparticle 1000 nm. The application of oil loaded silica nanoparticle was done against E coli biofilm system and biofilm quantification assay showed 81% reduction in biofilm. The inhibition was further confirmed using light microscopic analysis of biofilm after staining with 0.5% crystal violet. The study...
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Posted by admin on Nov 1, 2014 in |
CoII complexes of ligands pyridine3carboxamide thiosemicarbazone (L1), pyridine 3 carboxamide semicarbazone (L2), pyridine2carboxamide thiosemicarbazone (L3) and pyridine 2 carboxamide semicarbazone (L4) have been synthesized. The ligands were characterized based on elemental analyses, IR, 1HNMR studies while the complexes were characterized by elemental analyses, molar conductance, magnetic moment, IR, electronic and EPR spectral studies. The complexes are not soluble in ordinary organic solvent but highly soluble in dimethylsulfoxide. The conductivity data show the nonelectrolytic nature of the complexes. Based on elemental analyses, molar conductance data and other spectral studies the complexes were found to have general composition [Co (L)2X2] (where L=L1, L2, L3 and L4, X = Cl–, ½SO42–,NO3–) and exhibit distorted octahedral...
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Posted by admin on Nov 1, 2014 in |
The novel Co (II) and Zn (II) complexes have been synthesized by reaction of CoCl2/ZnCl2 with Schiff bases of benzoyl derivative of 3-methyl-1-phenyl-2-pyrazoline-5-one and sulfa drugs in 1:2 ratio. The synthesized complexes were screened/tested for their antimicrobial activity against pathogenic bacterial strains i.e. E. coli, Staphylococcus aureus strain I, Staphylococcus aureus strain II, Staphylococcus aureus strain III, Vibrio cholera, Gram positive Cocci, Gram positive Bacillus, Gram negative Bacillus, Bacillus subtilis and Salmonella typhimurium. It was found that metal complexes have more antimicrobial activity than their parent Schiff bases. Complexes of Schiff base ligand sulfamerazine and bmphp (L2) were also found to be more...
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Posted by admin on Nov 1, 2014 in |
The present study evaluates the anti-inflammatory potential of the methanol and chloroform extracts of Cleome viscosa and C. burmanni by in-vitro methods.The anti-inflammatory property of the extracts was assessed using albumin denaturation assay, proteinase inhibitory activityand hyaluronidase inhibition assay and the results compared against reference drugs such as Aspirin and Indomethacin. The present findings exhibited a concentration dependent inhibition of albumin denaturation and hyaluronidase enzyme by the extracts of both plants. Proteinase activity was also significantly inhibited by both plant extracts. The methanol extract was more effective than chloroform extract and was comparable to the standards.From the present study, it can be concluded that the extracts of C. viscosa and C. burmanni possessed marked in- vitro anti-inflammatory activity and the effect could be due to the presence of various phytochemicals present in the...
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