Posted by admin on Sep 1, 2014 in |
Background and Objectives: Glucose 6-Phosphate Dehydrogenase (G-6PD) is a ubiquitous enzyme playing a key role in the metabolism of glucose. Reduced activity of G-6PD is one of the most prevalent and genetically determined hereditary defects of the human being. Recently, it has been suggested that reduced G–6PD activity in pre-eclampsia may act as a contributory factor for the development of this complicated pregnancy. Material and Methods: The material for the present study comprised of 50 pregnant females at or after 20 weeks of pregnancy without complications and 50 pregnant women having blood pressure ³ 140/90 mmHg on at least two occasions ³ 4 h apart after 20 weeks of gestation. G-6PD Activity in RBCs was measured. Results: It was found that reduced Glucose-6-phosphate dehydrogenase (G6PD) enzyme activity may be a contributory factor for the development of preeclampsia in pregnancy. Conclusion: Reduced G6PD activity may be one of the causative factors for the development of preeclampsia, so, routine screening of erythrocyte G6PD activity during the antenatal period must be done....
Read More
Posted by admin on Sep 1, 2014 in |
Introduction: Global warming is one of the major issues in the current scenario. Heat stress is known to affect both physical and mental task. Imbalance in the maintenance of temperature leads to oxidative stress and damage to the body systems. Several studies have been documented on various stressors and its impact on antioxidant status. Aim: The present study was done to evaluate the antioxidant status in rats after acute heat stress. Methodology: Twelve male Swiss albino rats were randomly divided into two groups, control group, and experimental group. The experimental group of rats was exposed to acute heat stress at 38 ºC for four hours for a single day. Blood from the jugular vein was collected for the estimation of antioxidant statuses like LPO and SOD, GPx, CAT, vitamin C, and vitamin E in rats. Results: After exposure to acute heat stress, there was a significant and progressive increase (P<0.05) in LPO levels of the experimental group than compared to controls. Superoxide dismutase (SOD), Glutathione peroxidase (GPx) Catalase, vitamin...
Read More
Posted by admin on Sep 1, 2014 in |
An accurate and precise method was developed for Rosiglitazone Maleate in the visible range of electromagnetic spectrum sing Bromothymol Blue (BTB) in alkaline medium resulting in a blue chromogen showing its absorption maxima at 615 nm. Sandell’s sensitivity to the proposed method was found to be 1.5 × 10-4 µg/ml. The extracted complexes obey Beer–Lambert’s law in the concentration range of 1 to 30 µg/ml. The effect of the concentration of dye and pH have been studied and optimized. All the methods were validated as per ICH guidelines. The methods have been applied to the determination of drug in commercial tablets, and results of the analysis were validated statistically through recovery...
Read More
Posted by admin on Sep 1, 2014 in |
An attempt has been made to synthesize Benzoxazines and conduct Molecular docking studies of the same using Cyclooxygenase-1. All the synthesized compounds are then tested for anti-inflammatory activity by using fresh egg-white induced Paw edema method. The synthesis of novel Benzoxazine derivatives has been achieved by the reaction of Salicylaldehyde with Para Amino Benzoic Acid (PABA), which affords a Schiff base. The Schiff base on reduction with Sodium borohydride yields a reduced Schiff base which on cyclization with different aldehydes gives different substituted Benzoxazine derivatives. All the synthesized compounds are spectrally characterized by Infra-red, Mass, and 1HNMR spectroscopic techniques. The paw volume of the rat is measured with Plethysmometer. The % inhibition of paw thickness is calculated using the formula [% inhibition = (control-test)/control × 100]. All the synthesized compounds exhibited anti-inflammatory...
Read More
Posted by admin on Sep 1, 2014 in |
The present study deals with the phytochemical examination of therapeutic importance of Tiliacora acuminata (Lan) Hook. F & Thomas an important medicinal plant. Qualitative phytochemical analysis of the methanol and ethanol extracts prepared from Tiliacora acuminata stem and leaf revealed the presence of alkaloids, anthraquinones, catechins, coumarins, flavonoids, phenols, quinones, saponins, steroids, sugar, glycosides, tannins, and xanthoproteins. The FT-IR spectrum confirmed the presence of alkyl group, methyl group, alcohol group, ethers, esters, carboxylic acid, and anhydrides. The bioactive components of the ethanol extract of Tiliacora acuminata stem and leaf were investigated using Perkin-Elmer Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis of ethanol extract of stem of Tiliacora acuminata revealed the existence of 9,12,15-Octadecatrienoic acid (Z,Z,Z) (85.6%), 9,12,15,Octadecadienoic acid (Z,Z) (7.2%), Asarone (3.5%), Hexa-decanoic acid, ethyl ester (1.6%), Methyl-α-d-ribofuranoside (1.4%) and 9,12,15,Octadecadienoic acid, methyl ester (E,E)- (0.8%) and leaf of Tiliacora acuminata revealed the existence of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (14.9%),...
Read More
