Posted by admin on Nov 1, 2014 in |
The uses of plant medicines are very common in Asia and developing countries. Phytochemicals has been used to treat chronic as well as infectious diseases. Cardiospermum halicacabum Linn. (Sapindaceae) is an herbaceous climber, commonly found throughout India, used as vegetable as well as traditional medicine for rheumatism, lumbago and nervous diseases. Many pharmacological studies have been done with various parts of C. halicacabum plant to demonstrate its medicinal effect. The present work was aimed to isolate the active compounds from the leaf extract of C. halicacabum. The hydro alcoholic leaf extract (80% methanol + 20% distilled water) was subjected to isolation procedures for identification of active compounds. Eleven compounds were got and in vitro anti oxidant analysis were done with the isolated compounds.Compound 1 and 8 selected for structural elucidation. The NMR spectrum showed characteristic peaks of compound 1 and 8 and the active phytoconstituents were identified as Kaempferol- 3- o- α- l- rhamnoside and Apigenin – 7- o- β – d- glucuronide by structural elucidation. The isolated compounds...
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Posted by admin on Nov 1, 2014 in |
Antimicrobial effects of different extracts of lichens were evaluated in this study. Acetone extract of Heterodermia boryi have shown maximum inhibitory activity against Escherichia coli (22mm) followed by Staphylococcus aureus (21mm)and Pseudomonas fluorescenes (20 mm). Among fungi, diethyl ether extract gave desirable sensitivity pattern against Pestalotia foedans was very sensitive (21mm) followed by Paeciliomyces variotii (20mm). Most of the extracts of Sticta weigelii were found to be inactive against Fusarium oxysporum. Klebsiella pneumoniae was found to most resistant bacteria against Sticta weigelii. Among the tested microbial strains, Pseudomonas fluorescens was mostly inhibited (26 mm) by chloroform extract of Dermatocarpon vellereum. Serratia marcescens was found to be the most resistant strain against the lichen, but was effectively inhibited only by ethanolic extract of Dermatocarpon vellereum (20 mm). Hexane extract D. vellereum have shown maximum inhibitory activity against Staphylococcus aureus (23...
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Posted by admin on Nov 1, 2014 in |
A simple, rapid and precise stability indicating reverse phase high performance liquid chromatographic method has been developed for simultaneous estimation of Miglitol and Metformine Hydrochloride in their tablet dosage form. Chromatography was performed on a Phenomenex ODS C18 (250 X 4.6 mm) 5mm column using Water: Methanol (50:50, v/v) pH: 4 mixture as a mobile phase. The detection was carried out at 235 nm with a flow rate of 1.0 mL/min. The retention times were 4.807 and 3.273 minutes for Miglitol and Metformine Hydrochloride, respectively. The linearity of the method was excellent over a concentration range 2.5 to 7.5μg/mL for Miglitol and 25 to 75μg/mL for Metformine Hydrochloride. The correlation coefficient was 0.997 and 0.999 for Miglitol and Metformine Hydrochloride, respectively. The limit of detection was 0.6607μg/mL and 1.740μg/mL for Miglitol and Metformine Hydrochloride, respectively. The limit of quantitation was 2.0021μg/mL and 5.2736μg/mL for Miglitol and Metformine Hydrochloride, respectively. The relative standard deviation values for repeatability, intraday precision and interday precision studies were less than 2 % and %...
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Posted by admin on Nov 1, 2014 in |
A simple, rapid and precise reverse phase high performance liquid chromatographic method has been developed for simultaneous estimation of Cinnarizine and Dimenhydrinate in Tablet Dosage Form. Chromatography was performed on a PhenomenexPartisil ODS C18 (250 X 4.6 mm) 5mm column using Acetonitrile: Water (90:10) as a mobile phase. The detection of the synthetic mixture was carried out at 265 nm with a flow rate of 0.7 ml/min. The retention times were 4.3 and 5.8 minutes for Cinnarizine and Dimenhydrinate, respectively. Proposed method was validated as per ICH guidelines for linearity, accuracy, precision; specificity and robustness for estimation of Cinnarizine and Dimenhydrinate in Tablet Dosage Form and results were found to be satisfactory. The linearity of the method was excellent over a concentration range 20-100μg/ml for Cinnarizine and 40-200μg/ml for Dimenhydrinate. The correlation coefficient was 0.996 and 0.992 for Cinnarizine and Dimenhydrinate, respectively. The limit of detection was 1.62μg/mL and 2.62μg/mL for Cinnarizine and Dimenhydrinate, respectively. The limit of quantitation was 4.93μg/mL and 7.95μg/ml for Cinnarizine and Dimenhydrinate, respectively. The...
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Posted by admin on Nov 1, 2014 in |
The author selected and developed a new simple, sensitive and reproducible spectrofluorimetric method and validated for the determination of Memantine Hydrochloride in bulk as well as pharmaceutical formulations depending on condensation of Memantine hydrochloride with 1, 2 – Naphthoquinone -4- sulphonate (NQS) in an alkaline medium to become a product with orange color. The spectrofluorimetric method involved reduction of the product with potassium borohydrate and the later measurements of fluorescent formed lessen the NQS product after excitation at 295 nm and emission wavelength at 385 nm for the spectrofluorimetric method under optimum conditions, linear relationship with best correlation coefficient 0.9999 and the linearity was detected in between the range of 0.25-1.25 µg/mL. The mean accuracy was found to be 99.57 % to 99.66 %. The intraday and interday precision was found to be 0.14-0.16 % and 0.13-0.15% respectively. The limit of detection was found to be 0.0090µg/mL and the limit of quantification was 0.0270µg/mL. Therefore this newly recommended spectrofluorimetric method is most suitable for estimation of Memantine and observed...
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