Posted by admin on Nov 1, 2014 in |
A rapid and sensitive pH-gradient SAX HPLC method for the determination of Dermatan sulphate (DS) and trace levels of Oversulphated chondroitin sulphate (OSCS) in Heparin sodium was developed and validated. A pH gradient was opted to strike a balance between resolution and sensitivity because the response of OSCS was found to be better at neutral pH and the resolution between DS and Heparin was good at acidic pH. The use of commercially available lithium perchlorate was avoided and an in situ preparation of the same was opted to decrease the baseline drift which is a major concern for gradient methods using perchlorate mobile phases for compounds being analyzed at lower wavelengths. Chromatographic separation was achieved using a hydrophilic polymer resin-based SAX column, a linear gradientelution from 15% to 70% of solution-B which is 1.0M lithium perchlorate containing 5mM sodium dihydrogen phosphate at pH 6.0 and solution-A being 5mM sodium dihydrogen phosphate at pH 3.0 and a UV detection at 202 nm. The method has been validated and the limit...
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Posted by admin on Nov 1, 2014 in |
The aim of the study is to screen the grasses of South India for mineral nutrition. The results revealed that the grass species accumulate the major and minor elements at different concentrations. The maximum concentration (ppm) of macroelements in the analysed samples were 12270, 31310, 5309 and 3946 for Ca, K, Mg and P respectively. The maximum concentrations (ppm) of microelements were 50.05, 89.68, 2452, 258.3, 64.01 and 76.05 for B, Cu, Fe, Mn, Mo and Zn respectively. 22% of ash content and 4192 cal/g of calorific value were found. The levels of macro and micro elements determined of the grasses were analysed by using ICP –OES. Elemental studies of the grass species revealed that large amounts of major and minor elements were rich in Aristida hystrix and Echinochloa colonum, which may be suggested for livestock...
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Posted by admin on Nov 1, 2014 in |
Antibacterial activity of the two honey samples produced by the honey bee (Apis florea), was standardized by Disc diffusion method. The two honey samples were tested at three concentrations (5, 10 and 15µl v/v) and zone of inhibition were measured against Staphylococcus aureus, Escherichia coli, Basillus subitis, Pseudomonas putida and Klebsiela pneumonia; unifloral and multifloral honey samples were effected in inhibiting the growth of the gram positive and Gram negative organisms at (15 µl v/v). The inhibitory effect of unifloral honey on (15µl) Staphylococcus aureus and Escherichia coli; and multi floral honey on E.coli were comparable to that of Amphicillin (10µg/10µl). In conclusion, natural honey can be employed in treating, some common diseases especially those caused by the tested microorganisms and carry the potential of introducing new template into modern medicinal...
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Posted by admin on Nov 1, 2014 in |
By using five different sieve sizes of ascorbic acid granules, affects of granule sizes on tablet properties, i.e. tablet weight and hardness have been studied with standard methods. Affects of lubricant types and concentrations on tablet properties, i.e. tablet hardness and disintegration time have also been studied by using magnesium stearate and sodium stearyl fumarate as the lubricants for producing tablets from mixed sizes granules of ascorbic acid. Moreover, affects of high, moderate or medium and low compression forces on tablet hardness and disintegration time have been studied by using the mixed sizes granules of ascorbic acid. The result reveals that medium sized granules are better than smaller or larger granules to produce tablets within the specificity. Tablet weight variation has been found as higher during the production of tablets from smaller or larger granules. Production of tablets without lubricants has been complicated due to the difficulties in tablet ejection, resulting in picking, sticking, capping and cracking of tablets, higher tablet hardness and lower disintegration time. With increasing concentration...
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Posted by admin on Nov 1, 2014 in |
Endophytic fungi are ubiquitous in nature residing inside all the plant species contributing to their host plants by producing a plenty of substances that provide protection and survival value to the plant. Many researchers have proven that endophytes are a new and potential source of novel natural products for exploitation in modern medicine. This present study is undertaken to isolate and identify the potential endophytic fungi from Moringa olifera, a medicinal plants which is traditionally known and reported to posses various biological properties. A total of 72 fragments each 18 from the stem, leaves, flower and calyx were collected, surface sterilized and was inoculated on to Sabouraud dextrose agar (SDA) plates. Based on the macroscopic & microscopic features, the fungal isolates were identified.The most predominant endophytic fungal species isolated belonged to the genus Aspergillus spp. (53.3%) followed by Bipolaris spp. (6.6%). The Colonization Frequency (CF %) and Endophytic Infection Rate (EIR %) was found to be 83.31% and 20.83% respectively. The stem segments showed a maximum repository for endophytic...
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