Posted by admin on Jul 1, 2014 in |
Trichosanthes cucumirina L., locally known as “Chachindra” among the tribal and rural mass of Similipal Biosphere Reserve (SBR) forest. Plant parts are used as ethnomedicine among inhabitants of SBR. They use the fruits, leaves and roots against various diseases and traditional practices are continuing since long. Leaves are used against microbial infections, roots against diabetic and fruits against killing of stomach worm. Young fruits are used as vegetables as well as medicine. Keeping this traditional practice in view, assessment of food values and antibacterial properties were done. Fruits are rich with carbohydrate (26.24 %), lipid (2.20 %), protein (1.50 %), fiber (1.96 %) and good amount of moisture. Plant parts showed diverse bioactive compounds such as tannin, saponin, and flavonoid present in most of extracts of leaves, fruits and roots of T. cucumirina L. The antibacterial activity of plant extracts showed excellent against MTCC 1252, MTCC 1457, MTCC 3906, MTCC 1926, MTCC *497. Presents study highlights the nutritional, ethnobotanical and antibacterial activity of the plant and emphasis upon the...
Read More
Posted by admin on Jul 1, 2014 in |
To evaluate the protective effect of fenugreek (Trigonella foenum graecum L.) on alloxan induced diabetic mice, 15 male albino mice weighing 26-30 gm were used. They were divided into 3 groups. Control group received subcutaneous injection of 0.15 M acetate buffer for 15 days. Alloxan induced group received subcutaneous injection of alloxan 150 mg/ kg body weight to induce diabetes. Recovery group received subcutaneous injection of fenugreek seed extract 15 mg/kg body weight per day for 15 days. At the end of experiment histological and ultrathin sections of pancreas prepared. In diabetic mice disturbances in pancreatic arrangement is observed. While in electron microscopic study pyknotic nuclei, vacuolation and morphological changes in mitochondria, endoplasm reticulum and degranulation of β cells were observed. After treatment with fenugreek repair in pancreatic arrangement especially in β cells was clearly observed. In conclusion fenugreek seed extract could normalize the diabetes and provides protection to pancreatic tissues from damaging effect of...
Read More
Posted by admin on Jul 1, 2014 in |
Chronic Kidney diseases are increasingly recognized as a problem worldwide. Incidence of chronic kidney diseases is doubled in last fifteen years. The kidney is both cause and victim of hypertension. Hypertension is a serious risk factor for chronic kidney disease. The purpose of this study was to measure serum creatinine level in hypertensive patients as an indicator of kidney functioning and compared it with normal healthy controls. This study was carried out in Shanti Prapann Sharma Government Hospital, Rishikesh. In present study, the volunteers were selected from General Medicine department of Government hospital Rishikesh from May 2009 to May 2010. A total of 120 volunteers were recruited for this study. Out of them 70 were hypertensive subjects (38 males and 32 females) and 50 were normotensive subjects (27 males and 23 females). Hypertension was defined as per the recommendations of JNC7th Report. After 12 hours fast the blood samples were collected with the help of trained laboratory technician from all individuals without anticoagulant and centrifuged at 3000 rpm for...
Read More
Posted by admin on Jul 1, 2014 in |
Biofilm are a matrix of microorganisms which are adhered to and colonized a surface. When formed they are very difficult to remove and act as a source of contamination in processing environments. As bacteria in biofilm exhibit enhanced resistance to antibiotics and clearance by the host immune system, the resistance of enteropathogenic bacteria to commonly prescribed antibiotics is increasing both in developing as well as in developed countries. Resistances have emerged even to newer, more potent antimicrobial agents. This study was under taken to investigate the presence of multidrug resistance producing biofilm forming Aeromonas hydrophila in human clinical samples. A total of 150 urine samples were collected from private hospital in Tiruchengode during the period of six month. Among these only 75 isolates were found to be positive for Aeromonas hydrophila. The Starch-Ampicillin agar were used as a selective presumptive isolation medium for the isolation of bacterial isolates and confirmed as Aeromonas hydrophila were determined by using standard biochemical analysis according to Bergey’s manual of systematic Bacteriology. Slime producing...
Read More
Posted by admin on Jul 1, 2014 in |
A simple, rapid, and sensitive high-performance liquid chromatographic method with UV detection has been developed and validated according to the ICH guidelines for the quantitation of Moxifloxacin Hydrochloride (MOX), Ketorolac Tromethamine (KET) in pharmaceutical dosage form. Chromatographic separation was carried out in a Zorbax eclipse plus, C18 column (250 mm × 4.6 mm; 5 µm particle size) with simple mobile phase composition of 10 mM Potassium dihydrogen phosphate buffer with Triethylamine (pH 3.14) and acetonitrile (40:60, v/v) at a flow rate of 0.5 mL min-1 where detector was set at 302 nm with a total run time of 8 mins. The method was linear over the concentration range of 40-100, µg mL-1 with a correlation coefficient of 0.9891 and 0.994. Limit of quantifications (LOQ) of 13.3, 26.3 and limit of detections (LOD) 4.4, 8.7 µg mL-1 for MOX, and KET respectively. Accuracy and precision values of both within-run and between-run obtained from six different sets of three quality control (QC) samples analyzed in separate occasions for both the analytes...
Read More
