Posted by admin on Jul 1, 2014 in |
Polycarpaea corymbosa has been used traditionally as medicine for the treatment of various ailments. In the present investigation an attempt has been made to quantify phenol, flavonoid and steroid in methanol extract of aerial and root parts of P.corymbosa Lam. by HPTLC method. Different compositions of the mobile phase for HPTLC analysis were tested in order to obtain high resolution and reproducible peaks. The result of HPTLC analysis authenticates the presence of phenols, flavonoids and steroid with several peaks having different Rf values. Our results revealed that at least three types of phenols were seen in aerial methanolic extract and one in root extract. Similarly five types of flavonoids and three types of steroids were found in the Polycarpaea corymbosa whole plant methanolic extract. Phenol and flavonoid were found to have higher concentration in the methanolic extract when compared to steroids. Therefore, from the above results, methanolic extract of P.corymbosa can be used as therapeutic agents to treat various disorders caused by free radical and chemical substances due to...
Read More
Posted by admin on Jul 1, 2014 in |
The present paper deals with synthesis and characterization of some new chromium (III) Schiff base complexes using microwave irradiation technique as well as conventional heating. The S∩N donor benzothiazolines, 1-(2-furanyl) ethanone benzothiazoline (Bzt1N∩SH), 1-(2-thienyl) ethanone benzothiazoline (Bzt2N∩SH) and 1-(2-pyridyl) ethanone benzothiazoline (Bzt3N∩SH) were prepared by the condensation of ortho-aminothiophenol with respective ketones in ethanol. The chromium (III) complexes have been prepared by mixing CrCl3∙6H2O in 1:1 and 1:2 M ratios with benzothiazolines. The structure of the ligands and their transition metal complexes were confirmed by the elemental analysis, melting point and molecular weight determinations, IR, 1H NMR, electronic, EPR spectral studies. On the basis of these studies an octahedral environment around the chromium (III) ionhas been proposed. The in vitro antimicrobial and in vivo antifertility activities of Schiff base ligands and their respective chromium (III) complexes were performed on pathogenic bacterial and fungal strains and male albino rats respectively. The results indicated that the complexes showed higher activity than the parent...
Read More
Posted by admin on Jul 1, 2014 in |
The aim of the present work was to develop and validate a novel, rapid, sensitive, accurate and specific RP-HPLC assay with fluorescence detection for the quantification of amlodipine besylate in rat plasma to be applied to preclinical studies. Flourescein was used as internal standard (IS) and the detection was carried out by an online wavelength switching which enabled detection of amlodipine & flourescein in the same method. The plasma proteins were precipitated by a single step protein precipitation extraction procedure using methanol. Mean recovery of amlodipine besylate from rat plasma was more than 85.0 % for 25 – 5000 ng/ml concentrations. The assay exhibited good linear relationship with an r2 of 0.999. Lower limit of Quantification limit (LLOQ) was 1.84 µg/ml of amlodipine besylate and accuracy and precision were over the concentration range 25 – 5000 ng/ml. Thus, the bionanalytical method for quantification of amlodipine besylate is novel, since use of fluorescence detection & using flourescein as the internal standard has made the method with comparable sensitivity to LC-MS/MS...
Read More
Posted by admin on Jul 1, 2014 in |
The present study was designed to evaluate antidepressant effects of fruit extract of Elaeocarpus ganitrus(75% ethanol)on albino mice. Albino mice of either sex, weighing 25-30g were divided into seven groups and each group contains six animals. The animals were housed in polypropylene cages (38×23×10cm3) with not more than six animals per cage. Group I, II and III were administered orally with distilled water, fluoxetine and imipramine respectively. Group IV, V, VI and VII were administered orally with different doses (20, 40, 60 and 80 mg/kg body weight) of Elaeocarpus ganitrus fruit extract (EGFE). Antidepressant effect of EGFE assessed by forced swim test. The (EGFE) (20 and 40mg/kg) significantly decreased the immobility time and it also increased climbing and swimming time significantly. Duration of immobility of mice increased (246.00±1.73) at high dose (80mg/kg) as compared to vehicle (228.00±4.28). It is concluded that the EGFE exhibit antidepressant effect in tested animal model at low dose but at high dose it is...
Read More
Posted by admin on Jul 1, 2014 in |
A new simple and sensitive reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Dutasteride and Tamsulosin hydrochloride following forced degradation studies as per ICH guidelines. The degradation products were observed in acid and alkaline hydrolysis for Dutasteride; whereas Tamsulosin hydrochloride showed degradation on exposure to light. All the degradants were separated by preparative thin layer chromatography and subjected to structural elucidation by mass spectrometry using electron spray ionization technique. The base peak of DTS with m/z of 529.1 was formed in positive ESI mode. The daughter ion of DTS was observed at m/z of 461.2. Two degradants with m/z of 546.2 and m/z of 527.5 were formed by acid hydrolysis to give a carboxylic acid derivative and another by dehydrogenation respectively. Degradation product with m/z of 573.3was di-sodium adduct of DTS and was formed only in alkaline hydrolytic condition. The base peak of photo degradant of TMS with m/z of 409.1 was formed in positive ESI mode showed same m/z value of the base...
Read More
