Posted by admin on Sep 1, 2014 in |
The present study is a simple rapid, sensitive, selective and reproducible RP-HPLC method has been developed for the estimation of Triclosan in dental formulations. In present work a simple, sensitive and specific method (RP-HPLC assay, stability indicating RP-HPLC) has been developed by using a phenomenex, BDS C18, column having 5 µm particle size and 150 mm × 4.6 mm in length and gradient mode, with mobile phase containing acetic acid buffer (pH 2.5, adjusted with formic acid) and acetonitrile in the ratio of 30:70. The flow rate was 1.2 mL/min and effluents were monitored by PDA detector at 281nm. Injection volume was 20 µL. The method is linear (1-50 µg/mL). The regression equation was y = 7311x-2307 (r2 = 0.997). The method was validated for linearity, precision, accuracy, ruggedness, and forced degradation studies were performed. Estimation of triclosan was done in 3 dental formulations. Recoveries from formulations were between 98% and 102%. Limit of detection and limit of quantification was 0.101433 and 0.3333µg/mL, respectively. All the validation parameters are...
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Posted by admin on Sep 1, 2014 in |
Subcutaneous implantation of the drug is known to be the first medical approach aiming to achieve prolonged and continuous administration of drugs. The purpose of the research was to achieve sustained delivery of Tramadol Hydrochloride from biodegradable Gelatin-Sodium Alginate polymeric implant. Implants were prepared by using Gelatin-Sodium Alginate polymer in two ratios 70:30 & 80:20% w/w by heating and congealing method and then exposed to formaldehyde vapor for different periods (3, 6, 12 & 24 h) for hardening. Implants formulated with 80:20 Gelatin-Sodium Alginate ratio and hardened for 12 h were chosen for further studies based on drug loading and release performance. Effects of different excipients were studied on drug loading efficiency and drug release profile. Morphology of implant matrices, as studied by SEM, supported the experimental results. The release kinetics of drug was evaluated by fitting the data in four different kinetic models, namely, Zero order, First order, Higuchi and Korsmeyer‐Peppas. Implants were found to follow Korsmeyer Peppas Model the best in most cases. Good correlations were obtained...
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Posted by admin on Sep 1, 2014 in |
Many of the plants used to treat certain diseases because they have shown antimicrobial activity. In this case, many studies have conducted on antimicrobial activity of Artemisia annua. The crude extracts obtained from Artemisia annua L. (Asteraceae) were investigated for their antibacterial activity by using agar well diffusion assays against three bacterial species (Escherichia coli, Bacillus subtilis, and Salmonella typhi). The purpose of this study is to determine the antimicrobial activity of ethanol, methanol, and hexane extracts of Artemisia annua during different flowering stages. The zone of inhibition was calculated. Results indicate that the different concentrations of various extracts under study exhibit antimicrobial activity among different microorganisms. When compared to three organic solvents ethanol extract showed maximum zone of inhibition. Results proved that the parts of Artemisia annua might be potential sources of new antibacterial agents. At post flowering stages of the plant, the maximum zone of inhibition was observed when compared to the pre-flowering stage of the...
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Posted by admin on Sep 1, 2014 in |
Aim: H1 Antihistamines are classified into the first generation and second generation agents. The main differences between the first and second generations of drugs are their propensity to cause central nervous system (CNS) side effects. Therefore, the present study was aimed to analyze the effects of different H1 antihistamines (first and second generation) on CNS using different animal experimental models. Materials and Methods: H1 antihistamines such as pheniramine maleate (3 mg/kg, 6 mg/kg), cetirizine (0.6 mg/kg, 1.2 mg/kg), levocetirizine (0.6 mg/kg, 1.2 mg/kg), loratadine (1 mg/kg, 2 mg/kg) and desloratadine (0.6 mg/kg, 1.2 mg/kg) are evaluated and compared for their effects on CNS using experimental animal model (Pentobarbitone sleeping time, spontaneous motor activity, motor coordination) in Swiss albino mice. Results and Discussion: Desloratadine (0.6 mg/kg, 1.2 mg/kg) and loratadine (1 mg/kg, 2 mg/kg) did not produce significant (P<0.05) effect on sleeping time when compared to control. At 120 min time interval after treatment with cetirizine (1.2 mg/kg) and levocetirizine (1.2 mg/kg) was shown a reduction in locomotor activity and...
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Posted by admin on Sep 1, 2014 in |
A simple, accurate, precise RP-HPLC method has been developed for the estimation of Rizatriptan Benzoate. Good chromatographic separation was achieved by isocratic mode with Sodium dihydrogen orthophosphate as buffer (pH 3.5): Acetonitrile (80:20), as the mobile phase with Zorbax SB phenyl column (250 × 4.6 mm i.d, 5 µ particle size) as stationary phase with a flow rate of 1.0 ml/min at ambient temperature. Quantification was attained with UV detection at a wavelength of 255 nm. The retention time for Benzoate and Rizatriptan was 3.85 minutes and 4.32 minutes, respectively. The calibration graphs were linear in the concentration range of 5 – 25 μg/ml. The developed method was effectively applied to formulated fast disintegrated sublingual tablets of Rizatriptan Benzoate, and the % assay of the drug was found to be 96.4%. This method was statistically validated for the determination of accuracy, precision, specificity, linearity, range, the limit of detection, the limit of quantification and system suitability according to ICH...
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