Posted by admin on Sep 1, 2014 in |
Genistein is a soy phytoestrogen that mediates its functions by interacting with the endogenous estrogen receptor β. To investigate the effect of 10mg/kg body weight/ day genistein administration up to 90 days on the ovarian folliculogenesis and its correlation with serum gonadotropins and estrogen, adult Mus musculus were either administered with 1:4 DMSO : PBS (vehicle) or with 10mg genistein/kg body weight dissolved in vehicle for 30, 60 and 90 days. Post sacrifice on 31st, 61st and 91st day, blood serum was separated, and the gonadotropins (LH and FSH) and estrogen hormone were estimated by ELISA and Bouin’s fixed ovaries of control and treated mice were used for histology after H&E staining. Comparison of the ovarian sections revealed an increased number of arrested anovulatory follicles along with the treatment groups, i.e., 30 60 and 90 days and chronological decrease in the serum concentrations of LH, FSH as well as estrogens (two way ANOVA, at p<0.05 to p<0.001). Together these results suggested for delayed folliculogenesis, and reduced number of mature...
Read More
Posted by admin on Sep 1, 2014 in |
In the present study, the effort has been made to formulate the effervescent bioadhesive vaginal tablet of Metronidazole for localized treatment of Bacterial vaginosis. Among various bioadhesive polymers carbopol 934P, hydroxyl propyl methylcellulose K4M, methylcellulose, and sodium carboxymethyl cellulose were selected. Eight different formulations were made using a single polymer as well as a combination of two polymers in 1:1 ratio by direct compression technique. Formulations containing carbopol 934P alone (F4), carbopol 934P and sodium CMC in 1:1 ratio (F8) and carbopol 934P and hydroxypropyl methylcellulose K4Min 1:1ratio (F6) showed high, medium and low swelling behavior and bioadhesion property respectively and other formulations were found to disintegrate in 1 h time during swelling study as well as during dissolution study. In-vitro drug release was found to be 51.03% for F4, 42.98% for F8, and 34.87% for F6 at 12 h. F4 was found to release half of the total drug in 12 h. Based on swelling behavior, bioadhesion test, and in-vitro drug release, F4 was selected as the best...
Read More
Posted by admin on Sep 1, 2014 in |
The objective of the present study is to develop and validate a method for the estimation of gatifloxacin (GTX) in the tablet dosage form. An isocratic reversed-phase high-performance liquid chromatographic (HPLC) method was developed to estimate gatifloxacin using X terra C18 column (4.6 mm ´ 150 mm I.D., 5µm particle size) and the mobile phase containing phosphate buffer (pH adjusted to 3.5 with dilute phosphoric acid) and acetonitrile (60:40 v/v) at the flow rate of 0.5 ml/min. The UV detection was carried out at 298 nm. The retention time of gatifloxacin was found to be 2.340 min. The developed method was validated concerning accuracy, precession, linearity, selectivity, ruggedness, and robustness. Linearity for gatifloxacin was observed in the concentration range 0.01 to 0.50 µg/ml. Percent recovery was found to be 99.67. The results confirmed that the proposed method is specific, rapid, reproducible, and suitable for the routine determination of...
Read More
Posted by admin on Sep 1, 2014 in |
Swertia chirayita (SC) has been traditionally used for the treatment of several metabolic disorders related to health problems. We have investigated the antidyslipidemic and antioxidant potential of Swertia chirayita (SC) plant, a potent antidiabetic, and its isolated markers. The LC-ESI-MS standardized hot water extracts of different batches from the whole plant of Swertia chirayita, were tested in-vivo for their antidyslipidemic activity in Triton (WR-1339) and high-fat diet (HFD) induced dyslipidemic Charles Foster rats and in vitro for their antioxidant activity. Further solvent fractionation and isolation resulted in the identification of antidyslipidemic Mangiferin (MG) and Amarogentin (AM). Treatment of hyperlipidemic rats with MG and AM at 100 and 200mg/kg doses caused reduction in plasma levels of Low density lipoprotein (LDL) 24, 26% and very low – density lipoprotein (VLDL) 19, 25% as well as increase in levels of High density lipoprotein (HDL) 18, 22% respectively. In rats with HFD, MG and AM significantly reduced levels of VLDL lipids (26% and 27%) as well as Low-density lipoprotein-Total cholesterol (LDL-TC) 27% and...
Read More
Posted by admin on Aug 31, 2014 in |
The present study was designed to evaluate the oculohypotensive effect of enalaprilat in comparison to timolol using an intraocular pressure (IOP) recovery model. The study was carried out in 18 adult albino rabbits of 2.5-3.0 kg which were divided into 3 groups.Group Ireceivednormal saline,group II received enalaprilat (0.1%) and timolol (0.5%) was administered in group III. IOP recovery model was used to assess the effect of drugs on IOP recovery time. Additionally relative percent of IOP (IOPt%) at various time intervals was calculated by equation: IOPt% = (IOPt / IOP-40) x 100 % . Condition of conjunctiva was also examined. There was a significant (p= 0.006) delay of the IOP recovery with both timolol (150 ± 8.65 minutes) and enalaprilat (130 ± 6.83 minutes) when compared to normal saline (110 ± 6.83 minutes). There was also a change in relative percent of IOP at all the time intervals being significant at 80 minutes (p=0.036), 100 (p=0.006) and at 120 minutes (p=0.032). There was no congestion of conjunctiva with both...
Read More
