Posted by admin on Jul 3, 2014 in |
The development of a highly effective AIDS vaccine will depend on the success of designing immunogens that will elicit immune response against broadly neutralizing antibodies to naturally circulating strains of HIV- can be done by reverse vaccinology. Reverse vaccinology is a good approach for provoking the broadly neutralizing antibody response by identifying the specific epitopes i.e. surface proteins. The 3D structure of a protein is a prerequisite for structure based drug design as well as for identifying the conformational epitopes that are essential for the designing vaccines. The objective of this study is to identify peptides that can be used as potential vaccines against HIV using bioinformatics approach. B-cell epitopes which are present in the structural, regulatory and accessory proteins encoded by Clade-B HIV-1 genome were predicted using epitope prediction servers (RANKPEP) available in the public domains. Homology modeling approach was used to determine the 3D structures of these predicted epitopes. In addition to these studies, modes of binding of these epitopes to broadly neutralizing antibodies 2G12, B12, 4E10...
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Posted by admin on Jul 2, 2014 in |
Proteins, which are soluble under normal physiological conditions, sometimes form insoluble aggregates with serious medical implications. Bovine serum albumin (BSA), a structural analogue of human serum albumin. Bovine Serum Albumin (BSA) has been used as a carrier, and stabilizing agent, for insoluble fatty acids. The chemistry of chalcone has been recognized as a significant field of study. An interesting feature of chalcones is that they serve as starting materials for the synthesis of various heterocyclic compounds such as pyrimidines, pyrazolines, flavones, flavonols, flavanones, aurones and benzoylcoumarones as well as certain compounds like deoxybenzoins and hydantions which are of some therapeutic importance. A series of chalcones was synthesized by the Claisen-Schmidt condensation and the structures of 1-(4-aminophenyl)-3-phenylprop-2-en-1-ones were established with the help of IR and NMR study, then their effect was observed on bovine serum albumin. We have found that the synthesized chalcones interacted with bovine serum albumin and produce a great effect on their...
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Posted by admin on Jul 2, 2014 in |
A simple, accurate, rapid and sensitive high performance thin layer chromatographic method has been developed for the estimation of Tapentadol Hydrochloride in bulk and its tablet dosage form. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of methanol: toluene (4:1v/v). Densitometric evaluation of the separated zones was performed by UV detector at 272 nm. The drug was satisfactorily resolved with Rf value 0.5 ± 0.02.The method was linear over concentration range of 1500-2000 (1.5-2.0 µL) ng/spot. The analytical percent recovery was found to be 99.92%.The intra and interday precision with percent relative standard deviation (%RSD) values in the range of 0.0895 to 0.0892. The validation of the method was carried as per ICH guidelines. The proposed HPTLC method was successfully employed for routine analysis of bulk drug and its commercial tablet dosage...
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Posted by admin on Jul 2, 2014 in |
Eudragit RS100 microcapsules containing Aspirin as a model drug was prepared using solvent evaporation technique. The drug was dispersed in the organic phase and poured into the aqueous phase with stirring until microcapsules formation. The product was collected by filtration and air dried. The particle size analysis of the products was done and normal distribution curve showed for the same size range of different theoretical drug content, microcapsules had different size distribution. The sphericity of the microcapsule was greatly affected by the theoretical drug loaded. The mean microcapsule size decreased upon increasing the percent of theoretical drug content and then increased again. The surface of spherical microcapsule was smooth and no drug crystals attached to the surface. It was noticed drug crystals in the microcapsule structure. The mean actual drug content was markedly higher than the theoretical one. In the same product prepared with the same theoretical drug content, there was a relationship between the actual drug content and the product particle size. These findings were explained according to...
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Posted by admin on Jul 2, 2014 in |
Three simple, rapid, sensitive, specific and economic spectrophotometric methods were developed and validated for simultaneous quantitation of timolol maleate (TM) and brimonidine tartarate (BT) in bulk powder and eye drops. Vierordt’s and bivariate calibration algorithm spectrophotometric methods were developed for the simultaneous estimation of cited drugs in a binary mixture without previous separation. In the simultaneous equation method (Vierordt’s), TM and BT were quantified using their absorptivity values at selected wavelengths 257 nm and 295 nm, respectively. Also bivariate calibration procedure was successfully applied for simultaneous determination of both drugs. Difference spectrophotometric method was also tried and showed high selectivity for BT determination without any interference from TM or eye drops additives. The accuracy and reproducibility of the proposed methods were statistically validated by recovery studies. The calibration curves were found to be rectilinear over the concentration ranges 5-85 μg/mL for TM in all methods. BT calibration curves were rectilinear over the concentration (2–35 μg/mL) in case of D1 and Vierordt’s methods, while (5–35 μg/mL) in case of bivariate...
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