Posted by admin on Oct 1, 2014 in |
The present study investigated the antioxidant and antiproliferative efficacy of Andrographis paniculata, a well-known medicinal plant, using in-vitro systems. The dried leaves of A. paniculata were powdered and subjected to successive solvent extraction taking from polar to non-polar solvents (descending polarity) and simultaneously from non-polar to polar solvents (ascending polarity). Solvents used for extraction were hexane, chloroform, ethyl acetate, acetone, methanol, and water. All the extracts were screened for the presence or absence of various secondary metabolites. The antioxidant activity of all extracts at 100 μg/ml was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Further DPPH assay and reducing power assay were used to check the free radical scavenging capacity of methanolic and water extracts at their lower concentrations. The methanolic and water extracts of A. paniculata exhibited maximum DPPH inhibition of 20.75% and 17.71% respectively, at 100 μg/ml. A positive correlation between reducing power and concentration was found. Thin Layer Chromatography (TLC) of methanolic and water extracts of A. paniculata was performed. The antiproliferative effect of different concentrations of methanolic...
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Posted by admin on Oct 1, 2014 in |
Four wild plant species (Chrysthanthemum cornarium, Capparis spinosa, Carthamus lanatus, Haplophyllum tuberculatum) were investigated for their antioxidant activity, phenolic and flavonoid contents of their methanolic extracts. The antioxidant activity of the extracts was assessed their free radical scavenging properties using DPPH. Total phenolics and total flavonoids were detected by spectrophotometric and HPLC methods. The HPLC analysis compared with 14 reference standard phenolic compounds, results confirmed that gallic acid, chlorogenic acid, caffeic acid, 2,5- dihydroxy benzoic acid, and catechin were detected in the four methanolic plant extracts. HPLC chromatograms of the plant extracts showed that there was noticeable valuation in the distribution of phenolic compounds in the four studied species. Six bacterial strains were used in this study, three Gram-positive and three Gram-negative. Concerning with antimicrobial activity, results showed that plant extract of the studied species are a great source of phenolic compounds and represents the highest antibacterial activity against Gram-positive and negative bacteria. Results revealed that extracts represent the highest antibacterial activity against Gram-positive and negative bacteria and largest...
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Posted by admin on Oct 1, 2014 in |
Lantana camara L. is regarded as a notorious weed and has found several practices in folk medicine in many parts of the world. Leaves of Lantana camara L. is traditionally used for cuts, swellings, and burns for their healing property. The leaves are found to contain various chemical constituents such as monoterpenes, sesquiterpenes, triterpenes, and glycosides. In the present study, Ursolic acid stearoyl glucoside (UASG) isolated from Lantana camara L. (100 mg/kg/day) was evaluated for its wound healing activity in albino rats using excision and incision wound models. UASG treated animals exhibited significant (P<0.01 and P<0.001) reduction in wound area when compared to controls. UASG treated wounds are found to epithelize faster as compared to controls. Significant (p<0.01) increase in granuloma breaking strength was observed when compared with the control group. Histological section of the granuloma tissue of control animal showing lesser collagen formation indicated incomplete healing of the wound compared to UASG treated animals where complete epithelialization and increased collagen deposition occurred. The UASG showed significant wound healing...
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Posted by admin on Oct 1, 2014 in |
Plants have played a significant role in the treatment of cancer and infectious diseases for the last many decades. Natural products have been rediscovered as important tools for drug development despite advances in combinatorial chemistry. Bacopa monnieri (L.) Wettst. (Scrophulariaceae) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. The various ethnomedicinal uses mentioned are antitumor, against anxiety and depression, epilepsy, against bronchitis and asthma, gastrointestinal disorders, cardiovascular effects, hypothyroidism, anticonvulsant, analgesic, anti-inflammatory, antioxidative activity, antipyretic and antistress. Preliminary phytochemical analysis was carried out using different extracts. Sulforhodamine B (SRB) assay was done for in-vitro anticancer activity. The preliminary phytochemical studies of B. monnieri whole plant showed that it possesses tannins, alkaloids, steroids, saponins, glycosides, flavonoids, resins, amino acids, carbohydrates, fats, and fixed oils, proteins and starch. The in-vitro anticancer activity was performed against various Human Cancer Cell Lines namely Cervix (ME180, SiHa), Leukemia (HL60, K562), Ovarian (A2780, Ovkar-3), Breast (MCF-7, MDA-MB-468, MDA-MB-435, MDA-MB-231, ZR-75-1, BT-474), Prostate (PC3, DU145), Colon (HT29, Colo205), Lung (A549),...
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Posted by admin on Oct 1, 2014 in |
Trichosanthes cucumirina L., locally known as “Chachindra” among the tribal and rural mass of Similipal Biosphere Reserve (SBR) forest. Plant parts are used as ethnomedicine among inhabitants of SBR. They use the fruits, leaves, and roots against various diseases and traditional practices are continuing since long. Leaves are used against microbial infections, roots against diabetic and fruits against the killing of stomach worm. Young fruits are used as vegetables as well as medicine. Keeping this traditional practice in view, assessment of food values, and antibacterial properties were done. Fruits are rich with carbohydrate (26.24%), lipid (2.20%), protein (1.50%), fiber (1.96%), and a good amount of moisture. Plant parts showed diverse bioactive compounds such as tannin, saponin, and flavonoid present in most of the extracts of leaves, fruits and roots of T. cucumirina L. The antibacterial activity of plant extracts showed excellent against MTCC 1252, MTCC 1457, MTCC 3906, MTCC 1926, MTCC *497. Presents study highlights the nutritional, ethnobotanical, and antibacterial activity of the plant and emphasis upon the conservation...
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