Posted by admin on Aug 1, 2014 in |
An accurate, precise and reproducible UV-spectro-photometric methods and liquid chromatographic assay method were developed and validated for the determination of Levofloxacin and Ornidazole in tablet dosage form. Spectrophotometric estimation was done by simultaneous equation method and 50% methanol as solvent. In this method λmax for LEVO and OZ were selected at 293.5nm and 318nm. RP-HPLC analysis was carried out using Prontosil C-18 column (4.6 x 250mm, 5μ particle size) and mobile phase composed of Acetonitrile : 0.05% Ortho-phosphoric acid in water pH 3.0 (45:55% v/v)at a flow rate of 1.0 ml/min and chromatogram was recorded at 303 nm. Linearity was evaluated over the concentration range of 4 -20 μg/ml and 8-40μg/mL for LEVO and OZ in both UV spectrophotometric and RP-HPLC method (the value of r2= 0.999 found were by both the methods for LEVO and OZ). The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the both...
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Posted by admin on Aug 1, 2014 in |
The aim of this study was to extract the secondary metabolites of Coscinium fenestratum stem extracts and to determine the alkaloid berberine using HPLC. Phytochemical analysis was carried for acetone, chloroform, methanol and Aqueous fractions of stem extracts. Total phenolic content and total flavonoid contents were estimated using standard Gallic acid and Quercitin respectively. TLC and HPLC were performed for alkaloid to determine the berberine content present in the stem extract. Preliminary phytochemical screening revealed the presence of alkaloids, saponins, steroids, phenolic substances and cardiac glycosides. Steroids and tannins were absent in the stem extract. The stem methanolic extract of C.fenestratum contained large amount of phenolics18.35±0.56mg GAE/g extract and the flavonoid was found to be 12.8±0.88 mg QE/g extract respectively. The concentration of berberine in the stem was found to be...
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Posted by admin on Aug 1, 2014 in |
Diversity of plants has attracted a great deal of research interest in search of natural antioxidants and antimicrobials. Many natural substances having anti-oxidant and anti-microbial properties have been used in health foods for medicinal and preservative purposes Fleshy calyces ofHibiscus sabdariffa L. of family Malvaceae including the fleshy calyces were tested positive for presence of phytochemicals like Phenols, tannin, flavonoids, alkaloids, saponin, anthraquinone, triterpenoids and steroids etc. Both Petroleum ether extract and ethanol extract had shown similar antimicrobial activities. Petroleum ether extract was very effective against bacteria like Klebsiella pneumoniae, Staphylococcus aureus, Bacillus cereus and Lactobacillus brevis. But ethanol extract showed moderate antimicrobial activities against Staphylococcus aureus, Escherichia coli and Bacillus cereus. Antioxidants like phenolics and flavonoid content of roselle was found to be high. Petroleum ether extract also showed better antioxidant activity by scavenging DPPH free radical. This plant has the prospect of being a source of new clinically efficient antimicrobial or antioxidant...
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Posted by admin on Aug 1, 2014 in |
Background: With the dramatic advances in the medical science, treatment of many cancers is not just palliative, but rather curative in today’s world. The dosage regimen and the method of administration can greatly affect their efficacy and toxicity. Aims and Objects: To study the patterns of adverse drug reaction to cancer chemotherapy and to assess the causality, severity and predictability of those adverse reactions. Materials and Methods: The study was conducted in the department of Radiotherapy for a period of 6 months. It is a prospective study and included all in-patients & outpatients with adverse effects during the study period. The data was collected using ADR (adverse drug reaction) form designed by CDSCO (Centre for Drug Standard Control Organization) and statistical analysis was done using SPSS 20. Results: A total of 224 adverse reactions were noted in 178 patients with M:F ratio of 1:1.5. 54.5 % of the reactions were observed in age group of 40-60 years. The most common single drug causing reaction is cisplatin and regimen is...
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Posted by admin on Aug 1, 2014 in |
Aerva javanica belonging to family (Amaranthaceae) roots and flowers are reported to possess medicinal properties against rheumatism and kidney problems. The present work is to evaluate the nephroprotective effect of Aerva javanica by cisplatin induced renal toxicity in adult male albino rats of Wistar strain. The effect ofalcoholic extract and aqueous extract were evaluated at 200 mg/kg and 400 mg/kg, in rats against nephrotoxicity induced by administration of cisplatin through intra peritoneal route. Various serum parameters were studied along with histopathological examination of kidneys in each treatment group. Both the extracts were compared to the ursolic acid at the dose of 150 mg/kg as a standard drug. Alcoholic extract was foundto have significant nephroprotective activity. The levels of urea, creatinine, albumin and total protein in the serum were normalized after treatment with alcoholic extract at both the doses. The alcoholic extract at the dose of 400 mg/kg shows similar result as compared to the reference standard ursolic acid. The alcoholic extract of A. javanica possesses marked nephroprotective activity and...
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