Posted by admin on Jun 1, 2014 in |
Objective: To study detailed Pharmacognosy of the root of Operculina turpethum (Convolvulaceae) well known Ayurvedic drug (trivrit). Method: Macroscopy, microscopy, physicochemical analysis, preliminary phytochemical testing of the root and other WHO recommended methods for the standardization were done. Results: The periderm cells are tabular in shape; the phloem cells are homogeneous, tabular in shape and have suberized walls. The phelloderm cells are also tabular in shape and have cellulose walls. Irregular masses of squarish sclerenchyma cells are seen inner to the periderm and with outer cortical zone. Sclereids are also seen in small masses in the interior portion of the cortex. The cortical cells are densely loaded with starch grains and sparsely distributed calcium oxalate crystals. Secondary xylem is the thick and dense central cylinder comprising wide, circular thin walled vessels and thick walled lignified fibers. The vessels are solitary and diffuse in distribution. The xylem parenchyma cells are thick, tangential layered and they are apotracheal layered type. The parenchyma layers are away from the...
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Posted by admin on Jun 1, 2014 in |
A direct chiral separation method development was carried out for sitagliptin and its (S)-enantiomer on ten diverse chiral stationary phases. Chiral stationary phases, which were tested include, R, R Whelk-O1 column, macrocyclic glycopeptides namely, Chirobiotic R (Ristocetin A), Chirobiotic V (Vancomycin), Chirobiotic T (Teicoplanin), Chirobiotic TAG (Teicoplanin aglycone), OJ-H, OJ-RH belonging to tris (4-methylbenzoate) of cellulose. Chiral selectivity was observed on polysaccharide based CSP columns namely Chiralpak IA-3, Chiralpak IC-3 belonging to tris-(3,5-dimethylphenyl carbamate) of amylose, tris-(3,5-dichlorophenyl carbamate) of cellulose respectively and OD-H (tris-3,5-dimethylphenylcarbamate) of cellulose. Better enantioselective separation has been achieved on cellulose tris-(3,5-dichlorophenyl carbamate) column (Chiralpak IC-3), using IPA and n-hexane as mobile phase, both containing 0.05% ethylene diamine and at 0.5 mL/min flow rate. Detection was carried out at 266nm using PDA detector and column maintained at 35ºC. The method was validated for precision, accuracy, linearity and robustness. The advantages of the method are rapid equilibration and less solvent consumption due to short column length. Efficient enantio separation (Resolution 3.38) is due to small particle size...
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Posted by admin on Jun 1, 2014 in |
In present study the phytochemical analysis of the plant Acacia nilotica was done using GCMS method and seven compounds were identified as 3-picoline-2-nitro, 1-acetyl beta carboline, Hydroxy citronellal, Trans decalone, Propionic acid-2-chloro, ethyl ester, Lavandulyl acetate and D-Glucoronic acid. The anticancer activity of these seven compounds were analyzed with molecular docking study against the protein S-adenosyl-L-methionine decarboxylase which is found elevated in person with liver cancer. Among the seven phytochemicals identified, hydroxy citronellal followed by 1-Acetyl ß carboline was observed to have maximum score with S-adenosyl-L-methionine decarboxylase in molecular docking study, Glucoronic acid scores maximum in number of Hydrogen bonds. And taking into consideration all the scoring parameters, Glucoronic acid is considered as the best fit. Further studies are needed for the isolation of this particular phytochemical and subject it to further cell line testing and animal testing for anticancer activity and can be processed to be an effective and cheaper drug due to higher biomass availability of Acacia nilotica...
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Posted by admin on Jun 1, 2014 in |
The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of levofloxacin in bulk and pharmaceutical formulation. The solvent used throughout the experiment was 0.1N NaOH. Absorption maximum (λmax) of the drug was found to be 288 nm. The quantitative determination of the drug was carried out at 288 nm and Beer’s law was obeyed in the range of 2-12μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.0704x + 0.0034 with correlation coefficient R2of 0.999. The recovery values for levofloxacin ranged from 99.92% – 100.33%. The relative standard deviation of six replicates of assay was less than 2%.The percent relative standard deviation (RSD %) of interday precision range was 0.469 – 0.925 % and intraday precision range was 0.685 – 0.713%. The limit of detection and limit of quantification was 0.087μg/mL and 0.164μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.136 – 0.213%....
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Posted by admin on Jun 1, 2014 in |
ABSTRACT: Aim: The main aim behind this work is to study the effect of thermal methods used for the formulation upon the candesartan cilexetil properties and to evaluate the changes due to temperature Method: Melt method was followedto study the polymorphic changes in the candesartan cilexetil solid dispersions. In this method different ratios of drug to carrier were selected for the formulation. Initially the carrier was melted in china dish at 800c, when the complete carrier was converted to liquid form the weighed amount of drug was added to the liquefied carrier and stirred vigorously to get homogenous mixture and to get uniform coating of the carrier around the drug, then the homogenous mixture was cooled at room temperature to solidify the formulation. The final formulation was then evaluated by DSC, X-RD, FTIR, and HPLC to determine the polymorphic changes in the candesartan cilexetil. It was also evaluated to see whether there is any change in the release of the candesartan cilexetil. Results: Evaluating the final formulation of the...
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