Posted by admin on Jul 1, 2014 in |
A new simple and sensitive reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Dutasteride and Tamsulosin hydrochloride following forced degradation studies as per ICH guidelines. The degradation products were observed in acid and alkaline hydrolysis for Dutasteride; whereas Tamsulosin hydrochloride showed degradation on exposure to light. All the degradants were separated by preparative thin layer chromatography and subjected to structural elucidation by mass spectrometry using electron spray ionization technique. The base peak of DTS with m/z of 529.1 was formed in positive ESI mode. The daughter ion of DTS was observed at m/z of 461.2. Two degradants with m/z of 546.2 and m/z of 527.5 were formed by acid hydrolysis to give a carboxylic acid derivative and another by dehydrogenation respectively. Degradation product with m/z of 573.3was di-sodium adduct of DTS and was formed only in alkaline hydrolytic condition. The base peak of photo degradant of TMS with m/z of 409.1 was formed in positive ESI mode showed same m/z value of the base...
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Posted by admin on Jul 1, 2014 in |
The aim of the study was to develop and evaluate matrix based controlled drug delivery system of Bupropion hydrochloride. Controlled release tablets were prepared by employing Guar gum, Eudragit RS 100, HPMC K15M, HPMC K100M at different concentration. All 13 batches passed friability, hardness, weight variation, assay but only four batches (CRB7W, CRB8W, CRB10W, and CRB11W) passed the dissolution as per USP 30 NF25 for extended release tablet of Bupropion hydrochloride. Among the four formulation CRB7W & CRB11W follows Higuchi Model and CRB8W & CRB10W follows First order model. The optimized formulations were compared with the marketed product for similarity and dissimilarity factor. CRB7W and CRB10W showed dissimilar result and CRB8W and CRB11W showed similar result with marketed product. HPMC (K15M 7& K100M) as a matrix polymer used in tablet formulation provide a good initial retardation in the release as well as helped to enhance the overall release rate of the drug than that of Gaur gum and Eudragit RS...
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Posted by admin on Jul 1, 2014 in |
Woodfordia fruticosa (L.) Kurz. is a plant with vast medicinal properties. The plant and its parts are reported to be used for the treatment of hemorrhoids, dysentery, diarrhoea, liver diseases, piles, leucorrhoea, menorrhagia, ulcers, wounds, skin diseases, fever, headache, herpes, etc. The present work attempts to evaluate the physicochemical and preliminary phytochemical screening using various extracts of W. fruticosa (Family- Lythraceae). The herbal standardization was carried out on the basis of physico-chemical properties. Physicochemical parameters including ash values, extractive values and fluorescence analysis were evaluated. The qualitative phytochemical screening using various solvent extracts of plant revealed the presence of most of the biologically active phytochemicals in the methanolic extract of Woodfordia fruticosa leaves. TLC profiling of in vivo and in vitro plant part extracts gives an idea about the presence and confirmation of various phytochemicals. TLC screening showed the presence of ellagic acid (Rf 0.54), gallic acid (Rf 0.61), quercetin (Rf 0.8), myricetin (Rf 0.96) and β-sitosterol (Rf 0.58). Results confirmed the presence of therapeutically potent compounds in the...
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Posted by admin on Jul 1, 2014 in |
Proteomics is the large scale study of proteins. Before going to analyse the differentially expressed proteins, through 2-D gel electrophoresis the proteins were extracted in a sample under stress. In the present study, we were isolated the protein samples of E. coli Nissle 1917 treated with cocoti sap and wine stress. The isolated proteins washed with 2-D clean up kit because it was very sensitive to salts and detergents. Quantify the protein concentration for loading the samples in 2-D electrophoresis. Proteins were separated by based on IEF and Molecular weight. By using Image master 2-D platinum 6.0 software programmes analyze the protein spots in the gel. We noticed over all 800 proteins in our gel, 370 protein spots were visualized clearly, in that considers 15 spots were isolated based on the regulation. Ten spots shows up regulation and remaining shows down regulation. One newly expressed protein was isolated from wine treated gel when compared to the control and also sap treated gels. Independent t-test was performed to analyze the...
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Posted by admin on Jul 1, 2014 in |
Cultures of Aspergillus niger and Bacillus licheniformis were procured from NCL, Pune. Obtained cultures were exposed to UV irradiation. Mutants of Aspergillus niger exposed to 30 and 50 min UV and Bacillus licheniformis exposed to 20 and 30 min UV were selected. Growth of wild type and mutated Aspergillus niger and Bacillus licheniformis was found to be optimum at 48 hrs of incubation time, 30°C of incubation temperature and pH 6. SSF was carried out using two substrates namely banana peel and potato peel. Alpha amylase produced using the two substrates showed good activity but banana peel as a substrate exhibited highest activity of 113.6 (U/ml) in Aspergillus niger mutant exposed to 30 min UV treatment followed by banana peel exhibiting activity of 11.2 (U/ml) in Aspergillus niger mutant exposed to 50 min UV treatment. In this regard, production of amylase which can be used in food & pharmaceutical industry can be increased by using mutants & waste banana...
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