Posted by admin on Apr 1, 2014 in |
Helicteres isora (L.), a South-Asian plant, is a rich source of medicinal and antioxidant compounds and has been widely used in traditional medicine. Antioxidants are important to neutralize damaging free radicals in the body, especially when the systemic defence mechanisms prove insufficient. The objective of this study was to determine the antioxidant potential of its fresh and dry plant parts in various solvent systems. Plant material was collected from Karnala forest of Maharashtra. Extracts of leaves, bark, root and fruits (fresh and dry) were prepared using four different solvents viz. Distilled water, Ethanol, Methanol and Acetone. Each extract was tested for total phenolic content, total flavonoid content, and antioxidant activity by – FRAP, DPPH˙ and ABTS˙+ assays while phenolic compounds like Gallic acid, Cathechol, Vanillin, Caffic acid, p-Coumaric acid and Ferulic acid were detected using RP-HPLC. Antioxidant potential was significantly high in dried plant parts than the fresh ones. Leaves showed highest phenolic and flavonoid content (11.47 ± 0.50 mg GAE/g) and (54.16 ± 1.22mg RE/g) with 90.21 ±...
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Posted by admin on Apr 1, 2014 in |
Present studies was undertaken to find out of aquatic fungi in river Narmada. Fungi are eukaryotic, heterotrophic organisms, including both single-celled yeasts and multi-cellular filamentous fungi. Many fungal species can survive in oligotrophic environments, through scavenging nutrients from the substrate which they colonies, or the air or water in which they live. Fungi also produce secondary metabolites, some of which are toxins. Some of the fungal species and the metabolites they produce are human pathogens or allergens. It is an attempt for water quality studies by the analysis of aquatic fungi at three sampling sites in...
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Posted by admin on Apr 1, 2014 in |
A simple and precise RP-HPLC method was developed and validated for the determination of Choline fenofibrate in pharmaceutical dosage forms. Chromatography was carried out on Agilent make Zorbax C18 column (4.6 mm x 15 cm), 5 µ particle size using a mobile phase of phosphate buffer (pH 6.8) : acetonitrile (70 : 30 % v/v) at a flow rate of 0.8 ml/min. The analyte was monitored using UV detector at 298 nm. The retention time was found to be 3.207 min for Choline fenofibrate. The proposed method was found to be having linearity in the concentration range of 5-35 µg/ml with correlation coefficient 0.999. The mean recoveries obtained for Choline fenofibrate were in the range of 99.4-100.65 %. The developed method has been statistically validated according to ICH guidelines and found to be simple, precise and accurate with the prescribed values. Thus the proposed method was successfully applied for the estimation of Choline fenofibrate in routine quality control analysis in bulk and its...
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Posted by admin on Apr 1, 2014 in |
A series of novel hesperetin oxime esters (3a-l) were synthesized and there in vitro antioxidant potential was examined. Hesperetin oxime 2 was furnished by oximation of hesperetin then subsequently upon esterification with substituted benzoyl chlorides to obtain hesperetin oxime esters (3a-l) in good yields. The structure of compounds was elucidated by elemental analysis, IR, NMR (1H, 13C) and mass spectral studies. Among, the synthesized derivatives compounds (3i-l) showed pronounced antioxidant activity indeed higher than standard butylated hydroxyl anisole (BHA) and ascorbic acid (AA). Compounds with electronegative groups 3a and 3b demonstrated least activity compared to other...
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Posted by admin on Apr 1, 2014 in |
Onion, garlic and fenugreek are widely cultivated in Egypt and many reports confirmed their antidiabetic activities as used separately. Current study focused on these three herbs when present together in a mixture. All dried herbs were finely powdered to obtain homogenous mixtures. Pre-formulation studies were conducted on each individual herb including determination of foreign matter, loss on drying, acid insoluble ash and water contents. Analysis of active components responsible for the antidiabetic activity was also carried out using GLC and HPLC techniques. Pharmaceutical capsules were prepared by blending garlic, onion, and fenugreek seed powders in a ratio 1:1:2 respectively. The blend was granulated using 2% alcoholic solution of PVP k-30. The Disintegration testing of hard gelatin capsules was performed according to the USP, the capsules were disintegrated completely within 15 minutes in both distilled water and acetate buffer (0.05 M). In vitro release studies showed variable dependence of the active components on the pH of the release media. Pharmacological studies conducted on hyperglycemic rats indicated that both onion- and...
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