Posted by admin on May 1, 2014 in |
Aim: To evaluate the antidiarrhoeal activity of methanolic leaf extract of Anogeissus latifolia. Material and Methods: The methanolic extract was evaluated using rodent animal models like castor-oil induced diarrhoea and castor-oil induced enteropooling. The phytochemical constituents were also planned to screen by using standardized methods. The plant is traditionally used for the treatment of dysentery, snakebite, lepsory, diabetes, wounds and ulcers & skin diseases. Thus, the present study is therefore undertaken to investigate the antidiarrhoeal activity. Result: The methanolic extract of Anogeissus latifolia given orally at the dose of 200 and 300 mg/kg, showed significant antidiarrhoeal activity in castor oil induced diarrhoea by decreasing fecal episodes, and castor oil induced enteropooling by decreasing the volume of intestinal fluid. Conclusion: The results suggested that the extract may contain biologically active component that may be useful against diarrhoea, thereby validating its mention in the ancient Indian texts and justifying its use in ethanomedical...
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Posted by admin on May 1, 2014 in |
Hair loss problems can affect physical and mental health of human and have particular relevance during the aging process. In an effort to find solution of such problems, an attempt has been made to evaluate the hair growth promotion activity of a herbo-mineral Ayurvedic formulation, Trichup Tablet. Safety of drug was also evaluated by conducting acute toxicity study as per OECD guideline. For evaluation of hair growth promoting activity selected animals were divided in to three groups where each group was consisting of six animals. TED and TED×2 group was treated with Trichup Tablet at 198 mg/kg/day and 396 mg/kg/day, p.o., for 30 days respectively. Visual parameters, hair length, hair diameter and serum total protein were determined. The animals did not manifest any signs of toxicity or mortality. Treatment of Trichup Tablet showed significant effect on all visual parameters. Length and diameter of hairs were significantly increased in TED and TED×2. Serum total protein was also observed to be elevated in TED and TED×2 in comparison to normal control....
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Posted by admin on May 1, 2014 in |
This study investigates the level of cathepsin D activity in the serum and gingival tissues of periodontitis subjects and healthy individuals. The cathepsin D activity (spectrophotometric assay) was measured in the gingival tissues and serum of control healthy individuals (group I) and periodontitis subjects (group II). Inflamed gingival tissue was obtained from 15 periodontitis patients (9 males and 6 females) and15 control individuals (8 males and 7 females) aged 32-52 yrs with healthy gingival who were undergoing other forms of oral surgery. Serum was obtained by centrifuging the blood at 1500 r.p.m for 10 minutes. The enzyme unit was expressed by micromoles of tyrosine liberated per minute / 1000 ml in serum and micromoles of tyrosine liberated per minute / mg in gingival tissues. Cathepsin D activity in the serum and gingival tissue was higher in periodontitis group than that of the control healthy group (p < 0.05). We found that serum cathepsin D in the serum of group II subjects was elevated 8 times and in the gingival...
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Posted by admin on May 1, 2014 in |
Mucoadhesive buccal patches containing diclofenac sodium were prepared using the solvent casting method. HPMC used as bioadhesive polymer and different ratio of propylene glycol and glycerin also used. FT-IR and UV spectroscopic methods revealed that there is no interaction between Diclofenac sodium and polymers. The patches were evaluated for their physical characteristics like mass variation, drug content uniformity, folding endurance, surface pH, and in vitro drug release, in vitro buccal permeation study. Incorporation of DMSO generally enhanced the release rate. Swelling index was proportional to the concentration of HPMC. Optimized patches (F) showed satisfactory bioadhesive strength. The surface pH of all batches was within ± 0.4 units and thus no mucosal irritation is expected. Patches containing of F5 had higher bioadhesive strength with sustained drug release as compared to patches with other ratios of polymer. Data of in-vitro release from patches were fit in to different equations and kinetic models to explain release kinetics. The models used were zero and first-order, Hixon-Crowell, Higuchi and Korsemeyer-Peppas models equation. The optimized...
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Posted by admin on May 1, 2014 in |
Back ground: The study was designed to determine antiadipogenesis properties of crude methanolic extracts from four species of Caralluma R.Br. such as Caralluma adscendens (Roxb.) R. Brown var. attenuata (Wight) Grav. & Mayur. (CAA), Caralluma adscendens (Roxb.) R. Brown var. fimbriata (Wall.) Gravely & Mayur. (CAF), Caralluma stalagmifera C.E.C. Fisch. (CS) and Caralluma stalagmifera C.E.C. Fisch. var. longipetala Karupp. & Pull. (CSL) and as well as two species of Boucerosia Wight & Arn. such as Boucerosia lasiantha Wight.(BL) and Boucerosia umbellata (Haw.) Wight & Arn. (BU) at intervarietal and interspecific levels along with bioactivity studies. (BU) along with an isolated compound pregnane steroid using different cell lines. Methods: In vitro inhibitory activity of adipogenesis and facilitating activity on adipolysis were evaluated in 3T3 L1 adipocytes. Results: The percentage anti adipogenesis (50 µg/ml ) was also observed with values of 15.29%, 4.20%, 12.27%, 21.85%, 0 and 9.75% respectively. The percentage inhibition of pregnane steroid in adipogenesis of 3T3 L1 preadipocytes by 1 and 5 µg/ml were 21.22% and 35.97% respectively. Discussion: Four of...
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