Posted by admin on Feb 1, 2014 in |
Pseudomonas aeruginosa FARP72 was isolated from the skin mucus of freshwater catfish (Clarias batrachus) as a potent antagonistic bacterium. Efforts have made to assess the antagonistic activity against potential fish and human bacterial pathogens in vitro. Antagonistic effects were confirmed by cross-streaking method against pathogenic bacteria such as Aeromonas hydrophila, Bacillus subtilis, Bacillus amylolequifasciens, Escherichia coli, Edwardsiella tarda, Klebsiella pneumoniae, Enterohaemorrhagic Escherichia coli (EHEC), Pseudomonas aeruginosa, Salmonella Typhi, beta haemolytic Streptococcus, Staphylococcus aureus ATCC12598and Vibrio cholera. The LD50 value of Pseudomonas aeruginosaFARP72 and β-haemolytic Aeromonas hydrophila N10P were calculated to be >5.65×1010 cells/ fish and 2.37×108 cells/fish, which proved them to be non-pathogenic and moderate pathogenic respectively. In biocontrol experiment, the Labeo rohita stocks which received a first 15 min dip in Pseudomonas aeruginosaFARP72 suspension containing 1×107cells/ml followed by another dip in β- haemolytic Aeromonas hydrophila suspension containing 1×105 cells/ml for 15 min and subsequently cohabited with healthy Labeo rohita showed 37% mortality; while 75% of the fish died when treated only with β- haemolytic Aeromonas hydrophila suspension containing...
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Posted by admin on Feb 1, 2014 in |
In the present research work, four simple, precise, accurate and economical methods of UV spectroscopy have been developed for the estimation of Rilpivirine in bulk and pharmaceutical formulation. Method A involves estimation of Rilpivirine using low cost solvent, 0.01N HCl by zero order spectroscopy at an absorption maximum of 280nm. Method B involves Area under Curve method which involved calculation of integrated value of absorbance with respect to wavelength between two wavelengths selected, 275nm and 285nm respectively. Method C involves first order derivative technique for the same at 264nm. Method D involves second order derivative technique for the same at 237nm. The developed methods were found to be linear in the concentration range of 0.5-7.5μg/mL with correlation coefficient (R2) of 0.9998. The mean percentage label claim of tablets of Rilpivirine estimated by proposed methods was within the acceptable range. The developed methods were validated by following the analytical performance parameters suggested by ICH. All the validation parameters were within the acceptable range. As economical solvent is used, these methods...
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Posted by admin on Feb 1, 2014 in |
OrallyClopidogrel bisulphate has a short elimination half-life (7-8 hrs.), low oral bioavailability (50%) undergoes extensive first pass metabolism (85%) and frequent high doses (75 mg) are required to maintain the therapeutic level as a result, dose development toxic effect. The purpose of this research work was formulation and evaluation of transdermal drug delivery system of Clopidogrel bisulphate using vegetable oils as permeation enhancer by using solvent evaporation technique for improvement of bioavailability of drug and reducing toxic effects. Matrix transdermal patches were prepared by using hydroxypropylmethylcellulose (HPMC) and ethyl cellulose (EC) polymers by incorporating glycerine and dibutylphthalate as plasticizers, respectively. Prepared formulations were evaluated for different physicochemical characteristics like thickness, folding endurance, drug content, percentage moisture absorption, percentage moisture loss weight uniformity, etc.,. All the patches were uniform with respect to physicochemical evaluation. The in vitro drug release studies indicated that HPMC containing films have shown better release than that of EC containing films without any permeation enhancers. The result of diffusion study shows that formulation C7 showed maximum...
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Posted by admin on Feb 1, 2014 in |
The present study describes a simple, accurate and precise RP-HPLC Technique for the simultaneous determination of Flupirtine maleate and Paracetamol in pharmaceutical dosage form. The method involves an isocratic elution of drug in a stationary phase of Phenomenex, C18 (150mm × 4.6mm, 5µm) column using a mobile phase composition of methanol and 0.1% (v/v) orthophosphoric acid in the composition ratio of 60:40 v/v with a flow rate of 0.8 mL/min at 270 nm of detection. The injection volume is 20 µL. the method has been validated for specificity, linearity, range, precision, accuracy, limit of detection, limit of quantification, ruggedness and robustness. The retention times for Flupirtine maleate and Paracetmol are about 3.07 and 4.63 minutes respectively. Quantitative linearity was observed over the concentration range of 10.08 to 302.51 µg/mL for Flupirtine maleate and 4.99 to 99.80 for Paracetamol respectively. The regression equations of concentration of Flupirtine maleate and Paracetamol are found to be y = 1774x+4755, y = 39182x + 64154 respectively where y is the peak area and...
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Posted by admin on Feb 1, 2014 in |
1, 5-Dihydrobenzothiazepines are synthesized by conventional and microwave assisted synthesis methods. By microwave assisted synthesis, a considerable increase in the reaction rate has been observed and that too, with better yields. The compounds have been screened for antimicrobial and cytotoxic activity. 1, 5-Dihydrobenzothiazepines are prepared by the reaction of 1, 3-diarylprop-2-enones with o-aminothiophenol. All the products were tested for purity by tlc and characterized by elemental analysis, IR, 1H-NMR, 13C-NMR and mass spectral...
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