Posted by admin on Dec 1, 2013 in |
A simple, rapid, accurate, specific and sensitive reverse phase liquid chromatography method (RP-HPLC) was developed and validated for the estimation of Cytarabine in Pharmaceutical dosage form. The method was standardized using a Nova pack C18 column (250 mm x 4.6 mm ID; Particle size 3 μm) and the mobile phase consisted of acetonitrile: buffer (Ammonium acetate) at 30:70% v/v. The eluents were monitored at 272 nm and at 1 ml/min flow rate. The retention time was found to be 2.734 min for Cytarabine. The developed method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantification in accordance with the ICH guidelines. Linearity was obtained with the correlation coefficient value r2 =1 for Cytarabine. The proposed method can be used for the estimation of Cytarabine in bulk and Pharmaceutical dosage...
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Posted by admin on Dec 1, 2013 in |
A simple, precise, accurate and repeatable method for bulk and tablet dosage form of Ritonavir have been developed using spectrophotometric method. Differential spectrophotometric method was used. The developed method was validated according to ICH (Q2R1) guidelines and was found to be accurate, precise and specific. Amplitude difference was taken at absorbance maxima at 246 nm and absorbance minima at 266 nm. Linearity range was found to be within the concentration range of 10-30 μg/mL. Limit of detection and quantification was found to be 2.62 and 7.96 μg/mL respectively. The proposed method was found accurate in the range of 95.5 to 105.5%. It can be successfully applied for the estimation of Ritonavir in bulk and pharmaceutical dosage forms. Results of the analysis were validated statistically and by recovery...
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Posted by admin on Dec 1, 2013 in |
A novel fibrinolytic protease from Bacillus circulansGD25 was isolated and characterized for the fibrinolytic activity. The fibrinolytic protease was purified by ammonium sulphate fractionation (70%) and subjected to chromatographic methods like Sephadex G-50, DEAE –Sephadex A-50 columns. The purified enzyme has an approximately 38 kDa in size by SDS-PAGE and gel filtration. Optimum activity was at 35ºC and the enzyme was highly active over a wide range of pH from 7.0-9.0 with an optimum at pH 8.0. It exhibited stronger fibrinolytic protease activity. The activity was slightly enhanced by Ca2+ and Mg2+ whereas Zn2+, Cu2+, Co2+ and Fe2+ suppressed the activity. The fibrinolytic protease activity was totally lost in the presence of PMSF, suggesting that the purified enzyme is a serine metallo-protease with potential application in thrombolytic...
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Posted by admin on Dec 1, 2013 in |
In this study, an attempt has been made to approach of developing antibacterial finish using aromatic herbal oils. The five different herbal oils like Palmarosa, Petitgrain, Tea tree, Thyme, Lavender oil were screened with disc diffusion method against bacteria that normally exist like Staphylococcus aureus, Escherichia coli. The result showed good efficiency in Thyme oil when compared to other oils. Bamboo and Tencel fabrics are treated with thyme oil. The thyme oil finished fabric was assessed with anti- bacterial test (AATCC 147), anti-odor test (Organoleptic test) .The results indicates thyme oil treated fabric showed excellent antibacterial activity in both Bamboo and Tencel fabric compared to untreated sample. For anti-odor test Bamboo fabric shows good...
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Posted by admin on Dec 1, 2013 in |
The study was designed to determine the acute and sub-chronic toxic effects of aqueous extract of Gmelina arborea (Roxb.) in healthy male Wistar rats. An aqueous bark extract of G. arborea was administered at graded doses (0.25-2.00 g/kg) to Wistar rats in the acute toxicity test. Sub-chronic toxicity was evaluated by daily administration of the extract at 1.00 g/kg to Wistar rats for 30 days. All animals were physically active and no death was observed up to the dose of 2.00 g/kg in the acute toxicity study. The hematological parameters, serum concentration of liver enzymes and relative organ weight did not differ significantly in treated rats as compared to untreated healthy rats (P > 0.05). The histopathological study revealed no treatment-related gross cellular changes in vital organs in treated rats. The administration of the bark extract of G. arborea (1.00 g/kg) was found to be toxicologically safe in Wistar...
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