Posted by admin on Nov 1, 2013 in |
Two Simple, accurate, precise and sensitive spectrophotometric methods for estimation of Montelukast sodium (MON) have been developed and validated. The zero order spectroscopic method (Method I) and area under curve method (Method II) have developed. The proposed methods are validated according to ICH Q2B guidelines. The Montelukast sodium gives maximum absorbance at 241nm in chloroform and observed linearity 2-20 μg/ml for zero order method and 4-20 μg/ml for area under curve method. The stability of drug in chloroform has been studied and drug shows good stability in Chloroform. The recovery by method I and method II are 99.52 ± 0.069, 101.93± 0.11 respectively. The both spectrophotometric methods can be applied for routine analysis of Montelukast sodium in tablet formulation and in bulk...
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Posted by admin on Nov 1, 2013 in |
The present study was conducted to evaluate the hepatoprotective activity of ethyl acetate extract of stem bark of Crataeva magna (CM) in CCl4 induced toxicity in Wistar albino rats. Stem bark of CM were collected, and subjected to continuous hot extraction in a Soxhlet apparatus, for 72 h with solvents like chloroform, ethyl acetate and distilled water separately. Liver damage was induced in rats by administering CCl4 subcutaneously (s. c.) in the lower abdomen in a suspension of liquid paraffin (LP) in the ratio 1: 2 v/v at the dose of 1 ml CCl4/kg b. wt. of each animal. CCl4 was administered twice a week, on every first and fourth day of all 14 days. The extract at the dose of 200 mg/kg and 400 mg/kg b. wt. was evaluated by inducing hepatotoxicity with CCl4 and using silymarin (100 mg/kg) as the reference standard. Biochemical parameters like, SGOT, SGPT, SALP and serum bilirubin level were analysed. A section of liver was subjected to histopathological studies. Based on the above...
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Posted by admin on Nov 1, 2013 in |
In the present study, screened PGPR and Fungi were influence the growth of Mung bean (Vigna radiata) plant in the pot. Two rhizobacteria viz. Rhizobium sp., Pseudomonas putida and three fungiAspergillus niger, Rhizopus sp. and Trichoderma viride were isolated and purified. The effect of inoculation of different strains of bacteria and fungus on growth responses of Vigna radiata under pot condition was enumerated. The result revealed that the single and dual inoculation of these microbial strains enhances the plant growth in terms of root and shoot length and dry-biomass. The maximum increase in root length (up to 86.57%), shoot length (up to 56.91%), root dry weight (up to 94.42%), and shoot dry weight (up to 56.09%) was observed in response to dual inoculation of Pseudomonas putida with Trichoderma viride compared to uninoculated...
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Posted by admin on Nov 1, 2013 in |
A series of different 6-Methyl – 4- aryl -5 – (5-aryl -1,3,4-oxadiazole-2-yl ) -1,2,3,4-tetrahydro pyrimidin-2- (1H) – one / thione ( IIIa-g) have been synthesised fromEthyl – 6- methyl- 2- oxo / thioxo – 4-substituted phenyl-1,2,3,4-tetrahydro pyrimidine-5-carboxylate (Ia-g) followed by reaction with hydrazine hydrate in ethanol gave 6 – methyl-2-oxo / thioxo – 4 -substituted phenyl – 1,2,3,4-tetrahydro pyrimidine-5-carbo hydrazide (IIa-g) by means of Microwave irradiation for 2-4 mins and giving excellent yield in short reaction time, are notable advantages of this method. The structure elucidations of all the synthesised compounds have been accomplished by elemental analysis, IR, NMR and Mass spectroscopic...
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Posted by admin on Oct 1, 2013 in |
Most of the traditional systems of medicines are effective but the need is just to validate them to assess the quality, quantity and purity of the drugs. To support the same a polyherbal Ayurvedic powdered formulation Shatavaryadi churna used as immunomodulator, galactagogue, aphrodisiac and rejuvenator was taken for pharmacognostical, physicochemical and elemental investigation. Shatavaryadi churna is the composition of five ingredients, i.e. powder of Asparagus racemosus Willd. tubers-1part, Tribulus terrestris Linn. fruits- 1 part, Mucuna pruriens (L.) DC seeds- 1 part, Withania somnifera Dunal. roots- 1 part and Chlorophytum tuberosum Baker bulbs- 1 part. Ingredients of the formulation, In-house formulation and Marketed formulation have been pharmacognostically, physicochemically and elementally characterized by microscopy, physical constant values and Energy Dispersive Spectroscopy. Selected parameters for the investigation were anatomical study; powder microscopy; Extractive values i.e. alcohol soluble, water soluble, chloroform soluble, ethyl acetate soluble and petroleum ether soluble; Ash values i.e. total ash, water insoluble ash, acid insoluble ash; moisture content value, foreign matter and elemental content. The set parameters were found...
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