Posted by admin on Dec 1, 2013 in |
The aim of the study is to evaluate the diagnostic DNA profiles of the assessment of semen analysis data with respect to male fertility potential. Seman analysis taken form 50 patients of known fertility and 50 suspended infertility were studied by conventional semen analysis by suitable methods. The acridine orange test was used to assess the integrity of sperm DNA. The data throw doubt upon the validity of an approach based on the number of deviations for the normal standard values defined by the WHO. The alternative approach as specific semen characteristics (particularly morphology) as the major predictor of fertility produced no beneficial results. The means of sperm concentration in fertile males was significantly higher than infertile males (62.7±18.7 million vs. 36.7±25.40 million). Total sperm motility of fertile males also was significantly higher compared to infertile (56.32±0.07% vs. 28.8±10.7%). Normal sperm morphology of fertile males was also significantly higher compared to infertile (24.5±8% vs. 12.87±4.6%). According to these results we can conclude that testing sperm DNA damage is helpful...
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Posted by admin on Dec 1, 2013 in |
Antibacterial agents are essential drugs for human health and welfare. Many pharmaceutical industries have produced a number of antibiotics and resistance to these drugs by microorganisms increased. This development of resistance has necessitated the search for new anti-bacterial agents. Medicinal plants like spices are considered as a good source of natural products for maintaining human health since decades. The use of these plant extracts with known antimicrobial properties can be of great significance in therapeutic treatments. The main objective is to study the antimicrobial activity of combined plant extracts and to screen their anti-bacterial efficacy with the individual plant extracts by using Disc diffusion method against 5 bacterial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Proteus vulgaris). Aqueous extracts like Infusions and Decoctions from the following plants were prepared: Syzygium aromaticum (Clove), Laurus nobilis (Bay leaf), Cuminum cyminum (Cumin) and the comparison is made between individual extracts with the combined mixture of infusions and decoctions. The combination of three infusions was found to be more effective...
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Posted by admin on Dec 1, 2013 in |
This study evaluated methanolic extract of Filicium decipiens (MFD) on reducing hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats. MFD was orally administered once a day after 3 days of streptozotocin -induction at 100,200 and 400 mg/kg for 45 day and the results showed that serum fasting blood glucose, Glucose – 6 – phosphotase , Fructose 1, 6 –phosphotase in hepatic tissues ,TC, free fatty acids , phospholipids, and TG in serum levels were significantly decreased, whereas Hb and HbCA1in blood and serum HDL, Glucokinase, Glucose – 6 – Phosphate Dehydrogenase in hepatic tissues and liver and muscle glycogen level were increased. The dosage of 400 mg/kg is more effective than that of 100, 200 mg/kg. These results suggest that the MFD possesses antidiabetic and hypolipidemic effects in streptozotocin -induced diabetic...
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Posted by admin on Dec 1, 2013 in |
A reversed-phase-liquid chromatographic (RP-HPLC) method was developed for the determination of Metronidazole (MTZ) and Ciprofloxacin HCl (CPX) in their bulk drug and marketed formulations. The separation was carried out using a mobile phase of 25mM Potassium dihydrgen phosphate buffer of pH 3.0 consisting of 0.15%v/v Triethylamine, 0.1% Phosphoric acid and Methanol in the ratio of( 60:40v/v). The column used was Zorbax C18 column (250mm x 4.6mm, 5 µm) with flow rate of 1 ml / min using UVD 17 Detector. Detection carried out at 320 nm. The retention times of Metronidazole and Ciprofloxacin Hydrochloride were found to be 3.7 min and 5.6 min, respectively. Developed methods were validated according to ICH guidelines. Linearity was observed at concentration range of 50-250 μg/ml for Metronidazole and 62.5-312.5μg/ml for Ciprofloxacin Hydrochloride followed by Beer’s law. The percentage RSD for the method precision was found to be less than 2%. The proposed method is precise, accurate, selective and rapid for simultaneous determination of Metronidazole and Ciprofloxacin...
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Posted by admin on Dec 1, 2013 in |
Aim: To evaluate safety and efficacy of diclofenac 75 mg/1ml (Dynapar AQ) administered as IV bolus versus diclofenac 75mg/3ml administered as IV infusion in patients with postoperative pain. Methods: 350 postoperative adult patientswere randomized to receive either treatment. Primary efficacy endpoints were time to onset of analgesia and postoperative pain intensity while secondary efficacy endpoints included degree of pain relief and global assessment by patient and investigator. The safety endpoints were pain intensity and grade of thrombophlebitis at injection site. Safety and efficacy endpoints were evaluated over 12 hour study period. Results: Both study drugs were safe and effective throughout study period. However, IV bolus route of Dynapar AQ produced significantly faster onset of analgesia, better improvement in postoperative pain intensity and pain relief upto 1 hour, lesser thrombophlebitis and lesser pain at administration site upto 8 hours. Also, global assessment by patient and investigator was significantly favorable towards Dynapar AQ. Conclusions: IV bolus route of Dynapar AQ is better alternative to IV infusion of diclofenac 75mg/3ml with rapid...
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