Posted by admin on Jan 1, 2014 in |
The antimicrobial activity of the individual extracts of Doliocarpus dentatus and Montricardia arborescens and combined extracts of both plants were investigated against bacterial strains E. coli, S. aureus and K. pneumoniae using the Disc/ Agar Diffusion method under asceptic conditions. The magnitude of the diameter of the zone of inhibition was used as an indication for antimicrobial susceptibility or potency. Aqueous and C2H5OH extract of Doliocarpus dentatus showed insignificant antimicrobial activities whereas the CH2Cl2 and EtOAC extract of Doliocarpus dentatus were antimicrobial. The area of zone of inhibition ranging from 68.3 mm2 to 89.4 mm2. However, all the extracts of Montricardia arborescens were antimicrobial against all three bacterial strains. The area of zone of inhibition ranging from 68.3 mm2 to 172.6 mm2. The combined extract also showed variation in potency against the three microbial strains with the area of zone of inhibition ranging from 180 mm2 to 368.6...
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Posted by admin on Jan 1, 2014 in |
The present study explores the chemical composition, antimicrobial and antioxidant activities of sweet basil (Ocimum basilicum). Sweet basil essential oil was extracted using a Clevenger type apparatus. The oil yield of sweet basil was found to be 0.171%. A total of 75 compounds representing 99.9% of Sweet basil oil were identified. Linalool (69.85%) was identified as the major component present in sweet basil oil, followed by geraniol (10.850%), 1,8-cineole (6.430%), _ bergamotene (1.635%) and geranyl acetate (1.350%). sweet basil essential oil exhibited strong antibacterial activity against all the bacteria tested except Pseudomonas putida and Pseudomonas aeruginosa. The strongest inhibition activity of Sweet basil was observed against Streptococcus pneumoniae 2 (60 mm), Hemophilus influenzae (45 mm), Candida albicans (45 mm), S. pneumoniae 1 (37 mm) and Aspergillus niger (35 mm). Total antioxidants content quantified in Sweet basil essential oil using a commercial kit were found to be 50.32±1.8 mM. The essential oil extracted from Sweet basil was found to have higher linalool content, antimicrobial and antioxidant activities than most of...
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Posted by admin on Jan 1, 2014 in |
To investigate an Ayurvedic compound formulation Phalatrikadi Kvatha (PTK) having seven plant origin drugs for quality control and standardization. This compound formulation is mentioned in Ayurvedic classics for the treatment of Kamala ~ hepatic disorders. Macroscopy, qualitative phytochemical analysis, extractive values in ethanol and water and thin layer chromatography (TLC) of the formulation was done. Physicochemical parameters include loss on drying, total ash, acid insoluble ash, sulphated ash, alcohol soluble extractive, water soluble extractive and preliminary phytochemical screening of various solvent extract (hexane, chloroform, ethyl acetate, methanol and aqueous). Phytoconstituents such as Tannins, Saponin, Flavonoids, and Steroids were found to be present. Further TLC of Alcoholic extract of Phalatrikadi Kvatha (PTK) was done for proper identification. The qualitative phytochemical and pharmacognostic study of the drug is helpful in sample identification, quality and purity standards of the plant materials. The result of the present study can serves as valuable source of information and provides suitable standards for identification of PTK formulation for future investigation and its...
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Posted by admin on Jan 1, 2014 in |
Floating matrix tablets of ranitidine hydrochloride were developed and evaluated to increase bioavailability by increasing gastric residence time and sustained release of drug in the upper part of gastrointestinal tract thereby diminishing side effects and enhanced patient compliance. The tablets were prepared by direct compression method, using polymers such as Hydroxy propyl methyl cellulose, carbopol 940, xanthum gum, oryza sativa husk, chitosan and cetyl alcohol. The formulations were evaluated for various physical parameters, buoyancy studies, dissolution parameters and drug released profile from all formulation batch F8 showed slow and sustained release of ranitidine hydrochloride over a period of 12 hours upto 95%. Optimized floating matrix tablets F8 showed no change in physical appearance, drug content, or in dissolution pattern after storage at 40±2°C/75±5% for 90 days. It was concluded that formulation F8 shows the better buoyancy and drug...
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Posted by admin on Jan 1, 2014 in |
A simple, accurate rapid and precise RP-HPLC method has been developed and validated for determination of Hamycin and Ketoconazole in Pharmaceutical Cream. The RP-HPLC separation was achieved on Thermosil C-18, (250 mm, 4.6 mm, 5μm) using mobile phase 0.4 % ( v/v) diisopropylamine in methanol (v/v): 0.5% (w/v) Ammonium acetate in distilled water (90:10 % v/v) pH 6.5 adjusted with Glacial acetic acid at flow rate of 1.0 ml/min at ambient temperature. The retention times were 2.433 min. for Hamycin and 4.711 min. for Ketoconazole. Calibration plots were linear over the concentration range 50-250 μg/ml for Hamycin and 200-1000 μg/ml. Quantification was achieved with UV detection at 263 nm over the Beer-Lambert’s range.The method was validated statistically and applied successfully for the determination of Hamycin and Ketoconazole. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for the routine determination of Hamycin and Ketoconazole in pharmaceutical cream. The proposed method was validated as...
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