Posted by admin on Dec 1, 2013 in |
Swertia chirata Buch Ham (Gentianaceae) commonly known as chiratais a perennial herb. The present study was undertaken to evaluate the immunomodulatory effect of methanolic extract of S. chirata aerial part on Swiss albino mice. Haemagglutination Test (Humoral antibody response) and Delayed-type hypersensitivity (DTH) response test and were done to investigate the effect of methanolic extract of S. chirata on humoral and cell mediated immunity responses and flow cytometric studies for the estimation of T lymphocytes (CD3 and CD19) and Th1 cytokines (IL-2, IFN-γ and IL-4). Levamisole and Cyclophosphamide were used as immunostimulatory and immunosuppressive standard drugs. The methanolic extract of S. chirata showed dose related decrease in primary and secondary antibody response and Delayed type hypersensitivity (DTH) response with the maximum decrease at 200 mg kg-1 b wt (primary antibody response- 5.41±0.24, secondary antibody response- 5.39±0.19 and DTH response- 0.62±0.05). It also showed dose dependent decrease in the production of T lymphocytes (CD3 and CD19) and Th 1 cytokines (IL-2, IFN-γ and IL-4). The maximum decrease in the production...
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Posted by admin on Dec 1, 2013 in |
Human Papilloma Virus (HPV) is the smallest virus in the world. HPV is a non-enveloped double-stranded circular DNA virus, which can widely infect human skin and reproductive tract and respiratory epithelium. HPV also has a close relationship with benign and malignant tumors. There is no universal HPV treatment. Although there is a vaccine called Gardasil that can prevent some diseases caused by HPV, it is not recommended for everyone and does not eliminate the chance of getting other types of HPV. Thus, the aim of the present study is to design a subunit vaccine against HPV using bioinformatics approaches. In order to achieve our objective, we have used B-cell and T-cell epitope prediction methods. The possible vaccine target was proposed by using the conserved sequence among the chosen L1 binding protein from twenty different sequences. Structure prediction of this sequence by PSIPRED revealed that one helix is present in the sequence. The use of immune-informatics has greatly revolutionized the field of vaccine research, discovery and development. It demands the...
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Posted by admin on Dec 1, 2013 in |
Currently there has been an increased interest globally to identify the plant origin with free radical scavenging properties and have great importance as therapeutic agents in several diseases due to oxidative stress. Lipid peroxidation of cellular membranes has also been implicated in various disease processes, including cardiac ischemia-reperfusion injury. In recent years, the use of natural antioxidants present in food and other biological materials has attracted considerable interest due to their presumed safety, nutritional and therapeutic value. Shorea robusta has wide range of pharmacological activity but the cytoprotective activity against oxidative damage has not yet been documented. The present study was investigated the cytoprotective activity of Shorea robusta extract (SRE). The results of the study indicate that the cytoprotective role of SRE may be related to counteraction of free radicals. The mechanism of cytoprotective is to be worked out in future...
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Posted by admin on Dec 1, 2013 in |
The aim of the study is to evaluate the diagnostic DNA profiles of the assessment of semen analysis data with respect to male fertility potential. Seman analysis taken form 50 patients of known fertility and 50 suspended infertility were studied by conventional semen analysis by suitable methods. The acridine orange test was used to assess the integrity of sperm DNA. The data throw doubt upon the validity of an approach based on the number of deviations for the normal standard values defined by the WHO. The alternative approach as specific semen characteristics (particularly morphology) as the major predictor of fertility produced no beneficial results. The means of sperm concentration in fertile males was significantly higher than infertile males (62.7±18.7 million vs. 36.7±25.40 million). Total sperm motility of fertile males also was significantly higher compared to infertile (56.32±0.07% vs. 28.8±10.7%). Normal sperm morphology of fertile males was also significantly higher compared to infertile (24.5±8% vs. 12.87±4.6%). According to these results we can conclude that testing sperm DNA damage is helpful...
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Posted by admin on Dec 1, 2013 in |
Antibacterial agents are essential drugs for human health and welfare. Many pharmaceutical industries have produced a number of antibiotics and resistance to these drugs by microorganisms increased. This development of resistance has necessitated the search for new anti-bacterial agents. Medicinal plants like spices are considered as a good source of natural products for maintaining human health since decades. The use of these plant extracts with known antimicrobial properties can be of great significance in therapeutic treatments. The main objective is to study the antimicrobial activity of combined plant extracts and to screen their anti-bacterial efficacy with the individual plant extracts by using Disc diffusion method against 5 bacterial strains (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Proteus vulgaris). Aqueous extracts like Infusions and Decoctions from the following plants were prepared: Syzygium aromaticum (Clove), Laurus nobilis (Bay leaf), Cuminum cyminum (Cumin) and the comparison is made between individual extracts with the combined mixture of infusions and decoctions. The combination of three infusions was found to be more effective...
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