Posted by admin on Aug 1, 2013 in |
Purpose: The current investigation was carried out to study the hematopathological changes in the developing red blood cells of neonate of Swiss albino mice exposed with lead acetate and vitamins. Methods: Lead acetate was administered orally at 8, 16, 32 mg /kg/BW to selected pregnant females from 10th day of gestation to 21st day of lactation. Vitamin C (166 mg/kg BW) and vitamin E (133mg/kg BW) individually and in combination with lead were also administered from 10th day during the same period. Hematopathological alterations in the RBCs were examined in neonates after birth at postnatal days 1, 7, 14 and 21. Results: The examination of blood smears demonstrated that lead exposure during gestation and lactation led to various hematological disorders in red blood cells of neonates and abnormal types of RBCs like schistocytes, stomatocytes, codocytes, echinocytes with prominent hypochromasia, anisocytosis, macrocytosis etc. were also noticeable. The antioxidants vitamin C and E also induced structural abnormalities independently and also with co-administration of lead in developing RBCs in forms of dacrocytes,...
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Posted by admin on Aug 1, 2013 in |
A simple, sensitive, rapid, accurate and economical polarimeteric method has been developed and subsequently validated for simultaneous determination of Sucrose – in Iron Sucrose Raw material, Iron Sucrose Injection and Inprocess Bulk sample of Iron Sucrose formulations in pharmaceuticals. The usual measure of purity of sucrose is by polarimetry – the measurement of the rotation of plane-polarized light by a solution of sucrose. The specific rotation at 20°C using yellow “sodium-D” light (589 nm) is +66.47°. Commercial samples of sucrose are assayed using this parameter. Readings are not affected by the molecular weight of the Iron Sucrose, the entire procedure takes much less time to perform. The developed method was compared with RP-HPLC method. The described method was linear over a concentration range of 10-70 mg per ml for the assay of Sucrose. Results of analysis were validated statistically and by recovery studies. The limit of detection (LOD) and the limit of quantification (LOQ) for Sucrose were found to be 0.076 and 0.231mg per ml. The results of the study...
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Posted by admin on Aug 1, 2013 in |
Vascular oxidative stress occurs in hypertensive states and the potential role of antihypertensive drugs as antioxidants is currently under review. Hydrogen sulfide (H2S) is a recently recognized member of gasotransmitters, which may act to decrease blood pressure in experimental hypertension. The present study evaluated the antioxidant potential of hydrogen sulfide in SHR. NaHS, donor of H2S, was subjected to a series of in vitro tests to evaluate its antioxidant capacity. For in vivo study Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were divided in 4 groups namely WKY control (I), WKY-NaHS treated (II), SHR control (III) and SHR-NaHS treated (IV). Groups II and IV received NaHS, 56 µM/kg i.p. daily for 4 weeks. Blood pressure, renal cortical blood perfusion and pulse wave velocity were measured in acute studies. Oxidative and antioxidant markers from plasma were measured at the end of 4 weeks. In vitro, NaHS was found to be a free radical scavenger, reductant and inhibitor of lipid peroxidation. In vivo, the SHR control rats had higher blood...
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Posted by admin on Aug 1, 2013 in |
Evaluation of the anti-hepatotoxic activity of methanol-dichloromethane (MDM) extract of Oroxylum indicum (OI) whole plant on carbon tetrachloride (CCl4)- induced hepatotoxicity in rat liver explant cultures (in-vitro) and chronic liver damage in rats (in-vivo). In toxicant (CCl4) treated explants culture set (in-vitro), the marker enzymes GOT, GPT and ALP level increased significantly (p<0.001) when compared to control set. Treatment of CCl4-intoxicated liver explant culture sets with MDM extract of OI whole plant at a concentration of 3.3 mg/ml attenuated the marker enzymes GOT, GPT and ALP levels in culture supernatants significantly (p<0.001) as compared to CCl4 treated explant culture sets. A significant hepatocellular damage as evident from significant elevation in serum activities of GOT, GPT, ALP, bilirubin concentration (total and direct) with decrease in albumin and total protein were found in CCl4: liquid paraffin (1:1) treated animals (in-vivo) when compared to normal values. The MDM extract of OI whole plant (200 mg/kg/day, p.o.) exhibited a significant reduction (p<0.001) in CCl4: liquid paraffin (1:1) induced increased serum levels of GOT,...
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Posted by admin on Aug 1, 2013 in |
The fruit of Phoebe cooperiana U.N Kanjilal ex A. Das is highly priced and one of the most preferred folk food among the Indigenous people of Arunachal Pradesh, India. The methanol extract of fruit collected from East Siang District of Arunachal Pradesh, India were evaluated for total phenolic content, total flavonoid content and antioxidant potential. The methanol extract of fruit were subjected to 2, 2-Diphenyl-1-picrylhydazyl (DPPH) assay for free radical scavenging property and ABTS radical cation scavenging activity to determine antioxidant potential. Folin-Ciocalteu’s method for quantification of total phenolic content and colorimetric method for quantification of total flavonoid content were used in the experiment. Ethnobotany, mode of cooking, demand of fruit in the local market and unscientific harvest from forest and resultant threat on the plant is discussed in the paper. The methanol extract contains considerable phenolic (33.93±1.14 mg GAE/g) and flavonoid (941.5 ±5.31 µMRE/g) but no significant correlation between the total content of phenolic and flavonoid and antioxidant potential was found as IC50 value of 94.6±1.35µg/ml was found ...
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