Posted by admin on Oct 1, 2013 in |
One of the obstacles to effective intratumoral gene delivery lies in low transfection efficiency of non-viral vectors. Thus, in this study we evaluate the application of Diethyl methyl chitosan (DEMC) in intratumoral gene delivery of pancreatic cancer. DEMC/ pEGFP nanoparticles are prepared by polyelectrolyte complexation. After nanoparticle characterization via atomic force microscopy (AFM), in in vivo experiments, nude mice are subcutaneously injected with AsPC-1 cell line and the created tumors are used to determine the effect of DEMC on i.t. gene delivery via fluorescence microscopy, flow cytometry and immunohistochemistry. Also the relation between tumor-injection volume ratio (TIVR) and percent of the transfected tumor cells was predicted via mathematics. Relative to the control, which is injected with plasmid alone, there is a transfection increase up to 15- folds with DEMC. This delivery system involves simple preparation procedures and can be injected directly into the site, hence should be a useful approach to plasmid-based gene transfer for pancreatic cancer local...
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Posted by admin on Oct 1, 2013 in |
A Simple, rapid, sensitive, accurate and precise spectrofluorimetric method was developed for the determination of tolterodine tartrate (TOL) in bulk powder and in pharmaceutical preparation. This method is based on measuring the native fluorescence of TOL in methanol at 313 nm after excitation at 285 nm using 1 ml of phosphate buffer pH 6. All variables that affect fluorescence intensity such as diluting solvents, pH, buffer and buffer volume were studied and optimized .The fluorescence-concentration plot was rectilinear over the range of 0.5-8 µg/ml with a lower detection limit (LOD) of 0.090 µg/ml and lower quantification limit (LOQ) of 0.273 µg/ml. The method was validated and successfully applied to the determination of TOL tablets with an average percent recovery ± RSD% of 100.57 ± 0.76. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and...
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Posted by admin on Oct 1, 2013 in |
A simple, rapid and validated high performance liquid chromatography (HPLC) method with UV detection for the quantification of atorvastatin Calcium microemulsions (ATC-MEs) in rat plasma was developed. The assay procedure involved chromatographic separation using a C-18 chromatographic column (250 mm × 4.6 mm, i.d., 5 μm) and a mobile phase of methanol/water (containing 0.05 % glacial acetic acid) (70:30, v/v) 1.0 mL/min and detection wavelength of 248 nm. Plasma sample (200 μL) pretreatment was based on simple deproteinization by ethanol and centrifugation. The organic layer was evaporated under N2 gas, reconstituted with 200 μL of mobile phase, and 20 μL portions of reconstituted sample were injected onto the column. Calibration curve was linear (r ≥ 0.9854) from 0.1 to 50 mg/L. Both intra- and inter-day assay precisions that are presented through RSD were lower than 12.4% for intra-day and lower than 12.5% for inter-day assessment. This method was omitting the use of expensive solid phase extraction and time consuming liquid extraction procedures. Moreover, the present method was successfully applied...
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Posted by admin on Sep 1, 2013 in |
Emesis is a clinical condition which requires immediate treatment. The present work is aimed to formulate Orodispersible Tablet (ODT) of BCS class II drug Domperidone using Kyron T-314 as superdisintegrant and Avicel 102 as disintegrant by direct compression method. The drug-excipients compatibility study was carried out by Fourier Transform Infrared (FTIR) and Differential Scanning Calorimetry (DSC) which reveals no interaction between drug and excipients. A 32 factorial design was used to investigate effect of independent variables viz. superdisintegrant (Kyron T-314) and disintegrant (Avicel 102) on dependent responses like friability, disintegration time and percent drug release. Sodium lauryl sulphate (SLS) was used in the formulation to aid the dissolution of drug. All the formulations were evaluated for hardness, friability, disintegration time and dissolution rate. The factorial batch F4 containing 1.5 mg Kyron T-314 and 5 mg Avicel 102 has shown better results for all evaluation parameters with drug release of 99.22% and disintegration time of 29 sec. Therefore, factorial batch F4 was selected as the optimized...
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Posted by admin on Sep 1, 2013 in |
Kamrup District of Asasm is bounded by Udalguri and Baksa districts in the north, Meghalaya in the south, Darrang and Kamrup Metropolitan in the east and Goalpara and Nalbari district in the west. The rural part of kamrup district is mostly inhabitated by different tribes such as Boro, Khoronia Kochari, Rajbonghi, and Nepalis. An ethno botanical survey was conducted during the year 2011-2012 in some selected villages to explore the medicinal plants used by the local traditional healers known as ‘bej’ and ‘Ohjha’ for treatment of diabetes mellitus. The data’s were collected using questionnaire and personal interviews with few local healers. The investigation revealed 56 species of plants belonging to 38 families which are used to cure diabetes. These traditional ethno medicinal plants are consumed either in form of juice, powder, or boiled extract of leave, stem, root, seed, fruit, bark, rhizome and flower. In some cases it was found that the whole plant is directly used as potent...
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