Posted by admin on Aug 1, 2013 in |
Aim: To evaluate the in vitro antioxidant, antimutagenic and cytotoxic property of hydro-alcoholic extract of Thymus vulgaris L. by subjecting it to free radical scavenging, Ames and MTT assay respectively. Methods: Powdered Thyme leaves were extracted in a Soxhlet apparatus using 70% ethanol to obtain a dry extract. The extract was assessed for its total phenolic, tannin, flavonoid and flavonol content using quantitative assays. The antioxidant potential of Thyme was determined using DPPH•, ABTS•+, FTC and metal chelating assays. The in vitro inhibitory effects of Thyme on lipid peroxidation of liver and kidney along with erythrocyte hemolysis were performed following standard procedures. Further, the antimutagenic property of Thyme was investigated using Ames assay against TA 98 and TA 100 mutant strains of Salmonella typhimurium. The in vitro cytotoxicity assay of Thyme was examined following the MTT assay against Hep 2 and Hep G2 cell lines. Results: The hydro-alcoholic extract of Thyme registered 91.42 ± 0.90, 63.23 ± 1.02, 55.01 ± 0.42 and 65.69 ± 0.43 mg/g extract of total...
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Posted by admin on Aug 1, 2013 in |
Objectives: Arformoterol is the (R, R) – enantiomer of formoterol, a short and long acting β-2 agonist bronchodilator and is distinguishable from the racemic (R, R/S,S) – diasteriomer of formoterol, which is most commonly used. Some in vitro data show significant bronchodilatation, inhibition of inflammation and marked baseline airway reversibility with arformoterol. Yet, there is a dearth of study literature demonstrating its role as immediate reliever medication, in patients with exacerbation of non-severe asthma. The aim of this study was to compare the efficacy and safety of arformoterol with salbutamol nebulisation therapy, as a rescue medication, in acute non-severe asthma, not requiring hospitalisation, at a tertiary care centre. Methods: 40 patients, with exacerbation of non-severe asthma, were randomly allotted to Group A=20 and Group B=20. After every 30 minutes, 3 doses of salbutamol or arformoterol nebulization were given, respectively. Peak expiratory flow rates (PEFR) were measured at the baseline and 6 min, after each dose. Results: In the demographic profile, there were comparable results, with no statistically significant difference...
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Posted by admin on Aug 1, 2013 in |
Ethanol extract of the leaves of Taxus baccata, Phyllanthus debilis, Plectranthus amboinicus were studied for their antimicrobial property against multidrug resistant bacterial strains (MDR) from clinical specimens. Of these three plants, the most potent antimicrobial plant was Phyllanthus debilis followed by Taxus baccata (MIC range 80 – 400μg/ml) having broad spectrum activity, whereas Plectranthus amboinicus had no effect against MDR isolates. Taxus baccata and Phyllanthus debilis can be used as antimicrobial agents. Further confirmation needs ethano-pharmcological...
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Posted by admin on Aug 1, 2013 in |
The present study deals with the antioxidant activity, drug likeness score and bioactivity score of the compounds isolated from Curcuma longa and search lead antioxidant compound through molinspiration software. We have taken ten compounds from this plant. All the compounds were further subjected to molecular properties prediction and drug likeness by Molinspirstion and found in compliance with Lipinski’s rule of five. The compounds IV, V, IX, X fulfill Lipinski’s rule and show good drug likeness score. Milog P of these compounds was found below 5 that means these shows good permeability across cell membrane. TPSA below 160 Ǻ2, n violatios =1 or <0 it means compound easily bind to receptor, molecular mass <500, n rotb < 5 [10], Number of hydrogen bond donors ≤ 5 (The sum of OHs and NHs), Number of hydrogen bond acceptor ≤ 10 (The sum of Os and Ns). Compounds I-X were taken further calculation of bioactivity scoreby calculating the activity score of GPCR ligand, ion channel modulator, nuclear receptor legend, kinase inhibitor, protease...
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Posted by admin on Aug 1, 2013 in |
Olmesartan medoxomil (OLM) is an angiotensin II receptor blocker used for the treatment of hypertension. In this work an attempt was made to mask the metallic taste and enhance the solubility and dissolution rate of poorly soluble OLM by formulating it as inclusion complexes with β-cyclodextrins (β-CDs) as complexing agent in 1:1 molar ratio. The drug – CDs complexes were prepared by physical mixing and co-evaporation methods. The complex formation with in solid state was confirmed by Fourier transform infrared spectroscopy, x-ray diffractometry, differential scanning colorimetry and by scanning electron microscope analysis. From the prepared inclusion complexes, orodispersible tablets (ODTs) were formulated by using various superdisintegrants like sodium starch glycolate (SSG) and crospovidone in various concentrations (5-15%). Prepared tablets were evaluated for physical parameters and drug release by in vitro dissolution studies. ODTs containing SSG (15%) as super-disintegrant showed fastest disintegration and in vitro drug release. In conclusion, the present investigation demonstrates that the combination of inclusion complexation and using of superdisintegrants was a promising approach in the preparation...
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