Posted by admin on Nov 1, 2013 in |
A Simple, Rapid, Precise, Accurate, Robust and Stability indicating Reverse Phase HPLC method has been developed to estimate Capecitabine in tablet dosage form using mobile phase mixture consisting of Phosphate buffer : Acetonitrile (80:20) v/v at the flow rate of 1.2ml/min. The Hypersil BDS C8 column (250mm x 4.6mm x 5mm) was used as the stationary phase. The mobile phase was run for 15 min at the wavelength of 240 nm. The mean recovery was found to be 99.91%. The linearity range was 50% to 150% and was found to be successful under 0.999. The proposed method has fulfilled all the validation parameters such as linearity, Robustness, Accuracy, System Precision, intermediate precision, method precision, solution...
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Posted by admin on Nov 1, 2013 in |
A quantitative structure activity relationship study on series of total thirty three pthalimide analogues reported compounds was taken. Pthalimide analogues have several advantages over present ant diabetic drugs. The present drugs target insulin resistance and insulin insufficiency. So, it is believed that agents will be available alternative to other second line treatment options including sulfonylurea TZDs, DPP-4 inhibitors. Several statistical regression expressions were obtained using stepwise multiple linear regression analysis (MLR) and partial least square analysis (PLS). Pthalimide analogues activity is described by models that are built on simple 2D molecular descriptors and nevertheless are of good quality and predictive power. The results obtained after performing QSAR were; r2 = 0.8986, (MLR method) (equation-1), (equation-2) r2 = 0.6898 (PLS method) The parameters that are found to have significant correlation with hypoglycemic activity are Hosoya Index which is signifies the topological index or Z index, negatively contributing in the biological activity (~40%). The next descriptor is T_N_N_4 i.e. number of Nitrogen atoms (single double or triple bonded) separated from any...
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Posted by admin on Nov 1, 2013 in |
The purpose of this work is to evaluate the possibility of enzyme therapy through microencapsulation of serratiopeptidase (SP) in biodegradable nanoparticles of chitosan (CS). This drug has short biological half-life and thus frequent administration makes it a suitable candidate for controlled release. In this study, serratiopeptidase loaded chitosan nanoparticles were prepared by ionotropic gelation of CS with tripolyphosphate (TPP) anions. Reversible physical cross-linking by electrostatic interaction, instead of chemical cross-linking, has been applied to avoid the possible toxicity of reagents and other undesirable effects. The enzyme loaded particles optimized formulation was coated with sodium alginate solution to protect its release in stomach. The enzyme loaded nanoparticle formulations were characterized for by morphology, particle size, encapsulation efficiency and in-vitro drug release. The preliminary studies show that TPP and CS were compatible with SP. The ratio of CS to TPP has an influence on the mean particle size and when CS: TPP is 4:1 nanoparticles with smallest diameter are formed. Entrapment efficiency depends on the degree of deacetylation of chitosan. The...
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Posted by admin on Nov 1, 2013 in |
Ras proteins in association with GTP and GDP act as a bio-molecular switch for signaling cell growth, cell survival and signal transduction. The presence of mutated Ras proteins is found to vary in different cancer types and the highest occurrence of about 90% is observed in pancreatic cancer. The Ras GTPase binding site is mainly involved in signal cell proliferation. Hence, this binding site has been considered as a major target. At the same time, targeting a specific protein and designing the drug molecule with respect to that is practically of no use as the target proteins are fast mutating. In this scenario, designing the template from the hot spot of proteins and fitting the template for all the target protein molecules seem to be a promising technique. The templates are initially screened on the basis of pharmacokinetic and pharmacodynamic requirements. Six templates are found to be satisfying conditions like IC50, lipophilic efficiency, ligand efficiency etc. and their efficiencies are compared with standard reference molecules. The computed enrichment factors...
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Posted by admin on Nov 1, 2013 in |
Herbal medicines have been broadly used in developed countries hence they are natural and comparatively safe. They contain plant materials as their pharmacologically active components. Infertility is one of the most extremely tragic all over the world. Despite recent advances in the treatment of male infertility, the problem has not been satisfactorily tackled. The male infertility is mainly due to an inadequate number of spermatozoa in the semen, the failure of the spermatozoa to move with sufficient vigor towards their goal. Aim of the study is to evaluate the acute and sub-acute toxicity of the spermatogenic siddha drug Isappukol Chooranam (IC) (siddha drug).For acute studies, different doses of IC were administered orally to rats once daily for one week. Forsub-acute studies, different doses of IC were administered orally to rats once daily for 28 days in various doses at 50,100,200 mg/kg of body weight. Detailed hematological, biochemical, necropsy and Histopathological evaluation of organs was performed for all animals. Histopathological analysis revealed that Spleen, Testes, Pancreas, Lung, Liver, Brain, Heart,...
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