Posted by admin on Jun 1, 2013 in |
Reactive oxygen species (ROS) produced by activated macrophages play a major role in causing liver disorders. Small antioxidants and scavengers are protective only at very high concentrations and they poorly detoxify toxic ROS. Catalase (CAT) is an antioxidant enzyme that scavenges ROS and has great potential for treating liver disorders. In the present research work, gelatin nanoparticles bearing CAT were prepared using a two-step desolvation technique coupled with mannose and targeted in to liver to treat liver disorders associated with excess of ROS. It was characterized for shape, particle size, zeta potential, and percentage drug release and entrapment and confirmed by infrared spectroscopic studies. Experimental studies demonstrated that mannose Coupling enhanced their uptake into macrophages due to presence of mannose receptors in macrophages which increases their ability to scavenge ROS, produced by the activated...
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Posted by admin on Jun 1, 2013 in |
As part of a research initiative to evaluate plants used for their nutritional and herbal values, the antimicrobial activity of Solanum Melongena’s fruit and Moringa oleifera’s leaveswere investigated. Each plant part was subjected to selective extraction using solvents of varying polarity: n-C6H14, CH2Cl2, EtOAc and CH3CH2OH. The n-C6H14 and CH3CH2OH extract of these two plants were tested for their antimicrobial activity at three different concentrations of 5%, 10% and 20% of crude extracts against three pathogenic bacterial strains: Eschericia coli, Staphyloccocus aureus and Klebsiella pneumoniae using the Disc diffusion assay. Both n-C6H14 and CH3CH2OH extracts showed antibacterial activity at a higher concentration of 20% of crude extract. The order of bacteria susceptibility to Moringa oleifera extract been S. aureus > K. pneumoniae > E. coli whereas that for Solanum Melongena extract been S. aureus > E. coli > K. pneumonia. The area of zone of inhibition ranges from 44.15 mm2 to 53.55 mm2. These investigations suggest that the extracts of Moringa oleifera and Solanum Melongena can be used as...
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Posted by admin on Jun 1, 2013 in |
Antiretroviral therapy for decades is the appropriate treatment for infected populations with human immunodeficiency virus (HIV). However, it is associated with complications that affect the organism infected. The combination of antiretroviral therapy and pregnancy degrades iron metabolism. This work aims to evaluate and characterize the impact of the interaction between antiretroviral therapy and different stages of pregnancy in women of Abidjan on all biological indicators of iron status. The conducted investigations were a prospective, cross-sectional, descriptive analytical and case-control study. They concerned 275 women of reproductive age in all trimesters of pregnancy and in consultation to integrated center for bioclinical research of Abidjan (ICBRA). These women were divided into two groups: 135 pregnant women infected with HIV without antiretroviral therapy as controls and 140 pregnant women infected with HIV receiving antiretroviral therapy. All biological indicators of iron metabolism were determined either by colorimetric or immunoturbidimetric assays through blood samples from each pregnant woman or by calculation. The Student t test, factor analysis of variance (ANOVA) with two factors and...
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Posted by admin on Jun 1, 2013 in |
Solutions properties of sapide molecules provide fundamental information on type (hydrophobic or hydrophilic) and degree of hydration. Physico-Chemical properties like intrinsic viscosity, Apparent Specific Volume (ASV) and related parameters for bitter molecule (nicotine) and bitterness inhibitors (carbohydrate sweeteners, artificial sweeteners) and their mixtures were determined to study the role of water structure in the mechanism of unpleasant taste inhibition. The hydrophobicity of bitter molecule (nicotine) is manifested by ASV value of 0.920 cm3g-1 obtained in artificial buffered saliva (pH »8.2). Under these conditions nicotine (3-(1-methyl-2-pyrrolidinyl)pyridine is in neutral form, the most potentially hydrophobic. Experimental results indicate that viscometric constant (intrinsic viscosity [ƞ], Huggins constant k’, B coefficient) could discriminate between different sapid molecules tested at our laboratory. The ASV value of carbohydrate sweeteners falls within the sweet region. The bitterness of nicotine is manifested by ASV as high as 0.920 cm3g-1, which falls in the bitter region. Solution properties also determined for sweet-bitter mixtures validate an improvement in the fitting of solute molecules with solvent structure through the decrease...
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Posted by admin on Jun 1, 2013 in |
Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous estimation of Diclofenac Sodium (DIC) and Esomeprazole Magnesium Trihydrate in bulk drugs and synthetic mixture. Method A, Simultaneous equation method (Vierodt’s method) applies measurement of absorptivities at two wavelengths, 280.00 nm, (λmax of Diclofenac Sodium) and 301.00 nm,( (λmax of Esomeprazole Magnesium Trihydrate) in zero order spectra. The concentrations can be calculated from the derived equations. Method B, Absorbance ratio method (Q-method) involves formation of Q-absorbance equation at 302.80 nm (isoabsorptive point) and 280.00 nm (λmax of Diclofenac Sodium) in zero order spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 2.0 to 10.0 μg/ml for Esomerazole Magnesium Trihydrate and 5.0 to 25.0 μg/ml for Diclofenac Sodium with R2 value greater than 0.999. Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. Intraday and inter day precision was checked for both methods and mean %RSD was found to...
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