Posted by admin on Sep 1, 2013 in |
Fluvastatin sodium (FVS) is the first fully synthetic 3-hydroxy-3-methylglutaryl coenzyme (HMG-CoA) reductase inhibitors used for the treatment of hypercholesterolemia. It shows extensive first pass metabolism with short plasma half-life (3 hrs). The iontophoresis approach was used to determine the permeability of drug through isolated rat skin. In-vitro permeation of drug was determined by using glickfeld diffusion cell. Cathodal iontophoretic delivery was studied and optimized by evaluating donor compartment for the effect of pH, NaCl concentration, current density, pulsed depolarized DC current and drug concentration. Effectual permeation of FVS was obtained in phosphate buffer pH 5. Different concentration of NaCl showed negative effect on the iontophoretic permeation of drug. At higher current density, the rate of permeation was increased. Pulsed depolarized DC current showed higher flux (34.34 µg/cm2/hr) compared to the continuous DC current (26.44 µg/cm2/hr). With increasing concentration of drug, permeation was increased linearly. Comparison between human cadaver skin and rat skin showed that the permeation of drug was decreased from human cadaver skin (443.311±3.53 µg/cm2) as compare to...
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Posted by admin on Sep 1, 2013 in |
Asthma isa disease of the human respiratory system. The ethanolic extract of the roots of Picrorhiza kurroa was evaluated for antiasthmatic activity by using in-vivo and in-vitro model in guinea pigs. Animal studies used the histamine induced broncho-constriction in guinea pigs. The extract showed a maximum degree of protection of 52.16 % which was comparable to that of salbutamol 65.83 %. The effect of the ethanolic extract of the plant on isolated guinea pig ileum was studies to know the mechanism by which the extract exhibited muscle relaxant activity. The study showed the extract is effective against histamine and acetylcholine induced contraction. The extract exhibited relaxation effect against histamine and acetylcholine induced contraction at a concentration of 100 mg/ml. The result revealed that the ethanolic extract produced antiasthmatic activity due to presence of saponins and...
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Posted by admin on Sep 1, 2013 in |
Sustained released Aceclofenac microcapsules based on synthetic hydrophilic polymer hydroxypropyl methylcellulose (HPMC) were developed in this study in an attempt to design a dosage form that would manifest desirable release profile. W/O emulsion solvent evaporation technique was employed. Various release retarding agents were used to provide the analgesic and anti-inflammatory action of aceclofenac for prolong period of time. Microcapsules were prepared at a fixed stirring rate of 1000 rpm. Polymeric solution containing aceclofenac was emulsified by light liquid paraffin (LLP) which was initially emulsified by 1% (w/w of the continuum) lipophilic surfactant Span 80. The formulated microcapsules were evaluated for drug-polymer drug loading efficiency, micromeritic properties, surface morphology study, in-vitro drug release kinetics. It was found that all formulations showed satisfactory flow behavior. The maximum loading efficiency of was found to be 87.42%. SEM study showed that the microcapsules were discrete and spherical shaped. Efficacy of the dosage forms were evaluated in terms of in-vitro dissolution studies which showed statistically significant difference among the drug release profile from different polymeric...
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Posted by admin on Sep 1, 2013 in |
Tuberculosis is the major public health problem in throughout the world caused by Mycobacterium tuberculosis. Result indicates that micronutrient deficiency is common in patients with tuberculosis. As per in this study patients with newly diagnosed tuberculosis patients were divided into four groups. One group (n=41) received a placebo, second group (n=47) received 5000 IU vitamin A as retinyl acetate, third group (n=49) received 15 mg zinc as zinc sulphate and fourth group (n=41) received both 5000 IU vitamin A as retinyl acetate & 15 mg zinc as zinc sulphate. All groups received the same antituberculosis treatment (ATT). Results there were no significant differences in biochemical status amongst groups at baseline. After 2 months, the number of patients with sputum smears negative for tubercle bacilli was almost equal in all the groups (Group A-90.6%, Group B-90.3%, Group C-91.4% and Group D=93.1%). After 2 and 6 months of ATT, the increase in hemoglobin, serum albumin, vitamin A and zinc concentrations were significantly higher in the micronutrient group than in the placebo...
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Posted by admin on Sep 1, 2013 in |
A new, simple and sensitive spectroscopic method has been developed for the estimation of Atorvastatin calcium hydrate residue by using swabbing technique. In the proposed method the absorbance was measured 245.0nm corresponding to the absorbance maxima of Atorvastatin calcium in the ratio methanol and water as 90:10 as a solvent. Shimadzu-2450 UV-Visible spectrophotometer with UV Prove 2.31 software, capable of multi-component analysis, was used for quantization. Linearity range was observed in the concentration range of 1-10μg/ml for Atorvastatin. The % recovery of Atorvastatin was found out to be 100.2%. The method was validated statistically and recovery study was performed to confirm the accuracy of the method. The method was found to be rapid, simple, accurate and...
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