Posted by admin on Aug 1, 2013 in |
Actinomycetes species Streptomyces violaceorubidus, strain RC 1796 was isolated from Nagpur region which was identified from MTCC Chandigarh. The inoculum was prepared for fermentation using Streptomyces violaceorubidus RC 1796.Different fermentation parameters were studied to increase the yield which includes types of fermentation media, temperature, pH, agitation condition, time period of fermentation, inoculum size, and effect of different concentrations of fermentation media ingredients. The best media for fermentation was found to be yeast malt extract broth media. The optimum fermentation temperature was 28oC, pH 7, agitation speed 150 rpm; time period of fermentation 7 days, inoculum size 10%, concentration of media ingredients like yeast extract concentration 0.4% and malt extract concentration 1.0%. Analysis for the extracellular or intracellular antibiotic production was carried out and it was found that there was extracellular production of antibiotic. Production of antibiotic was done on large scale using the optimum conditions and by using yeast malt extracts broth for...
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Posted by admin on Aug 1, 2013 in |
The present study was designed to evaluate the antioxidant and anticancer activities of the ethanolic leaf extract of Ficus glomerata Roxb. on the basis of enzymatic and non-enzymatic antioxidants, xenobiotic enzymes and histopathological parameters in 7, 12- dimethylbenz(a)anthracene (DMBA) induced mammary carcinoma in rats. F. glomerata is large evergreen tree, found all over India and South East Asia. DMBA at a dose of 20mg/kg body weight (gastric intubation) significantly reduce the enzymatic antioxidant such as superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GR), glucose-6-phosphate-dehydrogenase (G6PD) and non-enzymatic antioxidant like vitamin-C, vitamin-E and total reduced glutathione (TRG) and lipid peroxidation (LPO) in group II rats. A significant increase in xenobiotic enzymes like cytochrome P450 and cytochrome b5, in DMBA induced groups were observed. Treatment with ethanolic leaf extract of F. glomerata (150mg/kg body weight, orally) in group III rats were observed all these above mentioned parameters were reverted to near normal level when compared to control rats. Finally, we concluded that the F. glomerata leaf...
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Posted by admin on Aug 1, 2013 in |
Fenugreek (Trigonella foenum-graecum) seeds were extracted in 80% methanol to examine the content of various potent antioxidant compounds and their influence on in-vitro oxidation of biomolecules i.e. amount. proteins, lipids and DNA. Fenugreek extract had polyphenols (9.47±0.10 mg GAE/g dry seeds) as major antioxidant principle. Gallic acid (170.335µg), caffeic acid (164.550 µg), ellagic acid (184.879 µg) and quercetin (215.814 µg)/g dry weight fenugreek seeds were identified in methanolic extract by HPLC analysis. Other antioxidant compounds ascorbate, tocopherol and riboflavin were present in very low amounts. Antioxidant properties were assayed using DPPH free radical scavenging activity. Fenugreek extract inhibited the metal induced oxidation of proteins and lipids. Presence of the extract could protect DNA against H2O2 -induced oxidative stress. IC50 value of fenugreek seeds was estimated by these mechanisms. The results of this study indicate that polyphenol rich methanolic extract of fenugreek had efficient free radical scavenging, reducing and metal chelating activity to protect biomolecules like proteins, lipids and DNA against oxidative...
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Posted by admin on Aug 1, 2013 in |
Two simple, accurate, novel, safe and precise methods developed for the simultaneous estimation of poorly water-soluble drugs Levofloxacin and Ornidazole in tablet dosage form using 2M sodium acetate and 8M urea solution (50:50% W/W) as mixed hydrotropic solution. Sodium acetate and urea solution did not show any absorbance above 240 nm and thus no interference in the estimation of drugs were seen. LEVO and OZ follows the Beer’s law in the concentration range of 5-25 µg/ml (r2= 0.9997 and 0.9998). Method-A simultaneous equation method employs 287 and 320 nm as two analytical wavelengths, method-B is absorption ratio method, which uses 301 and 320 nm as two analytical wavelengths were used for estimation of LEVO and OZ. The mean percent label claims of tablet dosage were found to be 98.528±0.431 and 97.916±0.732 in method A, 97.586±0.821 and 98.642±0.293 in method B for LEVO and OZ respectively. The standard deviation, coefficient of variance and standard error were obtained for LEVO and OZ was satisfactorily low. The developed methods were validated according...
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Posted by admin on Aug 1, 2013 in |
Floating matrix tablets of Metformin hydrochloride were developed and evaluated to increase bioavailability by increasing gastric residence time and sustained release of drug in the upper part of gastrointestinal tract thereby diminishing side effects and enhanced patient compliance. The tablets were formulated by using central composite design having two independent variables at three levels. Independent variables were total amount of hydrophilic gel forming polymers (Hydroxyl Propyl Methyl Cellulose K15M and Carbopol 934P) X1 and polymer-polymer ratio X2. The prepared formulations were evaluated for various physicochemical, buoyant and in-vitro drug release characteristics. All formulations possessed good floating properties with total floating time more than 12 hours. Statistical Optimization carried out for various responses like ‘n’ of Peppas equation, ‘K’ of zero order and ‘n’ of higuchi equation and T80%. Optimized formulation was found to float for longer duration and provided more sustained release of the drug. Release kinetics of optimized formulation followed Higuchi model with anomalous non -fickian diffusion. Hence Optimized floating matrix tablet could be a promising delivery system...
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