Posted by admin on May 1, 2013 in |
Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous analysis of Paracetamol (PCM) and Tapentadol (TAP) in their combined dosage form. Method A, First order derivative method involves measurement of absorbance at 257.060 nm (for TAP) and 289.406 nm (for PCM) in first order derivative spectra. Method B, Second order derivative method involves measurement of absorbance at 238.391 nm (for TAP) and 279.381 nm (for PCM) in Second order derivative spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 1.0 to 5.0 μg/mL for Tapentadol and 6.5 to 32.5 μg/mL for Paracetamol with R square value greater than 0.997 (for Method A) and 0.998 (for Method B). Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. Intraday and inter day precision was checked for all methods and mean %RSD was found to be less than 2 for all the methods. The methods were successfully applied for estimation...
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Posted by admin on May 1, 2013 in |
Andrographolide is one of the main active principles in Andrographis paniculata .It exhibits enormous pharmacological effects. Due to this immense value it required more level in pharmaceutical to satisfy the global need. Because it present in a very low level in normal regenerated plant of Andrographis paniculata. Micro propagation is a proven method for efficient invitropropagation of medicinal plants and for commercial exploitation of valuable plant derived pharmaceuticals. Hence in this present study aimed that to enhance the andrographolide content through hairy root culture techniques and elicitation process. Hairy root cultures induced in Andrographis paniculata used for determining the effect of signal compound on andrographolide production. Methyl jasmonate and salicylic acid were used in varying concentration and introduced into the 10d old hairy root cultures. HPLC studies are revealed that methyl jasmonate at 100 mM concentration shows higher efficacy (5fold/25d) than other increasing concentrations of same. Salicylic acid shows 8 fold (100mM) enhancements than hairy root cultures...
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Posted by admin on May 1, 2013 in |
The antitumor activity of methanolic extract of Decalepis hamiltonii was evaluated using Dalton’s Lymphoma Ascites (DLA) tumor model in Swiss albino mice. Methanolic extract of Decalepis hamiltonii was administered at a dose of 200mg and 400mg/kg b.wt once daily for 14 days after 24h of tumor inoculation. Cyclophosphamide was used as a standard drug at a dose of 25mg/kg b.wt. The result showed that methanolic extract of Decalepis hamiltonii root increased the mean survival time and percentage increase in life span and also decreased the body weight of the DLA tumor bearing mice. Hematological studies reveal that increase in hemoglobin content and RBC count and decrease in WBC count was observed in methanolic extract of root of Decalepis hamiltonii and cyclophosphamide treated mice. Administration of methanolic extract of root of Decalepis hamiltonii and standard control significantly decrease the serum marker enzymes (aspartate transaminase, alanine transaminase) and protein level to nearly normal levels in DLA tumor bearing mice. The methanolic extract of root of Decalepis hamiltonii at a dose of...
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Posted by admin on May 1, 2013 in |
Incorporation of drugs into non-ionic surfactant vesicles (niosomes) during their manufacture affords a possible method of achieving controlled release. The aim of this study is to formulate niosomes as carriers for delivery of diclofenac sodium (DCS). Niosomes are prepared with a series of sorbitan monoesters (Span 20, 40, 60, and 80) and sorbitan tri-oleate (Span 85) and co-surfactants of polyoxyethelene fatty acid esters (Tween 20, 40, 60, and 80), with or without cholesterol and charged lipids like stearylamine or dicetylphosphate. The prepared niosomes were evaluated for the entrapment efficiency (EE %) and in-vitro release rate of DCS. Niosomes formed of Span 60 gave the highest EE %, followed by Span 40, 20, 80 and 85. It was also found that increasing the total lipid concentration resulted in an increase in the EE %. However, the amount of encapsulated DCS (mg) per mmole lipid decreased. Increasing the concentration of DCS was accompanied by a slight increase in the EE % and a great increase in the entrapment efficiency expressed as...
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Posted by admin on May 1, 2013 in |
Non-alcoholic steatohepatitis (NASH) is a metabolic disorder of the liver that may progress to fibrosis or cirrhosis. In the present study, we examined the possible preventive effect of an extract of Teucrium polium L. (Lamiaceae), a plant with high flavonoid content and antioxidant activity, on experimental NASH. To induce NASH, a methionine and choline deficient (MCD) diet was given to N-Mary rats for 8 weeks. Rats were given normal diet (control, n=5), normal diet plus crude extract of T. polium (TPC) (0.5 g/kg body weight/day by gavages) (C+TPC, n=5), MCD diet (MCD, n=5) and MCD diet+ TPC (0.5 g powder/kg body weight/day by gavages). Histopathological evaluation of the liver samples revealed that treatment with TPC has abated the severity of NASH among the MCD–fed rats. In addition, the hepatic malondialdehyde, caspases–3 and TNF-α levels were decreased among the same group of rats. The activities of liver superoxide dismutase, glutathione peroxidase and the level of hepatic glutathione were also enhanced among MCD+TPC group relative to MCD group. Moreover, the crude...
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