Posted by admin on Jul 1, 2013 in |
A study was undertaken to fractionate and characterize the antidiabetic potential of Cyperus pangorei rhizomes. The column chromatography and HPTLC study was performed on ethyl acetate fraction and five sub-fractions were separated and subjected for the evaluation of antidiabetic potential in streptozotocin induced diabetic rats. The experimental data indicated that the ethyl acetate sub-fractions SF4 (26.39%) (P < 0.05) and SF5 (43.69%) (P < 0.05) at the dose of 50mg/kg BW produced the maximum fall in the blood glucose level of diabetic rats after 15 days of treatment. Elevated levels of triglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL), very low density lipoprotein (VLDL) and decrease level of high density lipoprotein (HDL) and serum insulin level were observed in Streptozotocin-induced diabetic rats. While significant differences were observed in serum insulin level and serum lipid profiles in sub fractions treated diabetic animals, when compared with the diabetic control and normal animals (P < 0.01). In sub fractions SF4 and SF5 treated group decreased level of serum TG, TC, LDL...
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Posted by admin on Jul 1, 2013 in |
An accurate, sensitive and precise RP-HPLC method has been developed and validated for the estimation of Glimepiride (GLM) from bulk drug and Pharmaceutical Dosage form. The separation was achieved by Hypersil C18 column (250mm X 4.6mm, 5μm) in isocratic mode, with mobile phase comprises of Acetonitrile : Buffer in proportion of 60:40v/v, buffer was 0.02M Potassium Di-hydrogen Phosphate (pH 4.5 adjusted with Ortho Phosphoric Acid). The flow rate of mobile phase was 1.0ml/min and employing UV detection with 232nm wavelengths. The retention time of GLM was 5.420 min.The calibration curve was found to be linear within the concentration range of 50µg/ml to 150µg/ml. The regression data for calibration curve shows good linear relationship with r2 = 0.9965. The method was validated in accordance with the requirements of ICH guidelines. Moreover, the proposed analytical method was applied to monitor the formulation commercially...
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Posted by admin on Jul 1, 2013 in |
A simple and rapid high performance thin layer chromatographic (HPTLC) method was developed for the assay of colchicine in bulk drug, pharmaceutical preparations and biological fluids. The method has a linear range of 4 – 35 μg/ml. The limits of detection and quantification were 1 and 4 μg/ml respectively. The results obtained by the proposed method were compared statistically by means of Student t-test and by the F-test with those of the reported method. The two were shown in good agreement. No interference was observed from common pharmaceutical excipients....
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Posted by admin on Jul 1, 2013 in |
An increased inclination has been observed for the use of herbal drugs in chronic and incurable diseases. Treatment of psychiatric diseases like Schizophrenia is largely palliative and more importantly a prominent adverse effect prevails with the majority of antipsychotic drugs, which are the extrapyramidal motor disorders. This study was a trial to evaluate the neuroleptic activity of the ethanolic extracts of Nardostachys Jatamansi with different antipsychotic animal models. Two doses of the extract (100 and 200mg/kg) were used for this study with 5 different animal models. After that, the concentration of the dopamine neurotransmitter was estimated in two different regions of the brain viz. Frontal cortex and Striatum. The result of the study indicated a significant reduction of amphetamine induced stereotype and conditioned avoidance response for the extracts compared with the control group, but did not have any significant effect in phencyclidine induced locomotor activity and social interaction activity. However the extract showed minor signs of catalepsy compared to the control group. The study also revealed that the neuroleptic...
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Posted by admin on Jul 1, 2013 in |
Synthetic analgesic drugs have major side effects like gastritis, gastric ulcer, kidney disorders and cardiac arrhythmias. So, use of natural or herbal analgesics is an important and growing part of the pain-control and new discoveries are made practically every year. Many of these drugs commit the side effects like Morphine is responsible do work against heavy sedation and may elevate mood in distressed patients as do the antidepressants. Caffeine, release the histamine in more amounts, also have stimulation. And the use of cannabis like a medicine remains a debated issue. Analgesic herbs are the therapeutic herbs with analgesic effect that are commonly known as pain relief herbs or simply the pain herbs. Analgesic herbs are natural pain reliever that reduce or eliminate pain. These pain relief herbs are available in the market in analgesic topical forms like essential oil and analgesic cream- that can be directly applied at the pain sites- as well as in form of capsules, tea and tinctures for pain relief. So, Author proposed the comparative...
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