Posted by admin on Apr 1, 2013 in |
Background: For many years, creatinine clearance has become increasingly popular because of several advantages. The major error arises in clearance test when the timing of urine collection is inaccurate and the bladder is incompletely empty. Aim: In view of the aforementioned controversial literature, it was decided to evaluate the relation-ship between the renal changes (creatinine clearance) in pre-eclamptic, eclamptic, preeclamptic toxeaemic, non-pregnant and normal pregnant women. Setting and Design: The present investigations were undertaken to study creatinine clearance in normal non-pregnant women, pregnant women in different trimesters, Preeclamptic toxaemic patients and eclamptic patients, between the age group 25-32 years admitted in Rajendra Institute of Medical Sciences, Ranchi during the period from June 2008 to September 2009. Methods and Material: Control group consisted of 15 normal non-pregnant women, 10 cases in first trimester, 30 cases in 2nd trimester, 20 cases in 3rd trimester, 60 cases of Preeclamptic toxaemia and 20 cases of eclampsia. Creatinine clearance measurements were done by enzymatic method and modified Jaffe’s kinetic method. Statical Analysis: Statistical analysis...
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Posted by admin on Apr 1, 2013 in |
Tannin acyl hydrolase, commonly referred to as tannase (E.C. 3.1.1.20), an inducible extra-cellular enzyme produced by a number of animals, plants and microbes. In this investigation, tannase production under solid-state fermentation by using Aspergillus niger and the waste residue of cashew husk was used as substrate for obtaining the desired fermented product. After termination of fermentation gallic acid was isolated from the tannase, and it was used in the production of an antibacterial drug Trimethoprim by using chemical and bioconversions. Microbial tannase is more stable than tannase from other sources like plants or animals. Tannase from fungal sources are reported to be active in a wide range of pH and temperature. Aspergillus niger was used in this study for the immobilization of tannase enzyme. Tannase production was reached maximum within 24 to 36 h against crude tannin extract obtained from Anacardium occidentale by using Aspergillus niger. Tannase assay was calculated. Activation energy of the immobilized enzyme was lower than that of the free enzyme. Optimum pH and temperature was...
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Posted by admin on Apr 1, 2013 in |
Purpose: The prevalence of allergic rhinitis (AR) is increasing at an alarming rate throughout the world. India has an estimated number of 15-20 million patients with allergic bronchial asthma and 30-80% of these suffer from AR. So, AR is considered as a major chronic respiratory disease due to its prevalence, impact on quality of life (QoL), work/school performance and productivity, economic burden and links with asthma. This research aims at testing the role of classical homeopathy in bringing changes in serum immunoglobulin E (IgE) level and absolute eosinophil count (primary outcome measures) and symptoms score and WHOQOL-BREF score related to AR (secondary outcome measures) by comparing the pre-trial and post-trial data. Method: An open label,single arm, experimental, prospective, non-randomized, non-controlled, before and after comparison pilot study was carried on 34 participants suffering from chronic AR. Institutional ethical clearance was obtained; then thirty four consenting patients were enrolled after screening of 58 patients by eligibility criteria and were allocated to classical homeopathic treatment. Four cases were drop-outs; thirty cases were...
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Posted by admin on Apr 1, 2013 in |
A simple, specific, precise, and efficient method for the Simultaneous estimation of Metformin HCl and Voglibose tablet by a Reverse Phase-High Performance Liquid Chromatography method is developed and validated. Selected mobile phase was in a combination of acetonitrile: buffer pH- 6.5 in the ratio of 62:38. Optimized column is a stainless steel column packed with base octa decylsilyl silica gel of 250X4.6mm and at 254 nm wavelength for metformin and voglibose detection by Spectrofluorimeter and excitation wavelength at 350nm and emission wavelength at 430nm. In our study the validation of analytical method for determination of Metformin and voglibose tablet formulation was performed in accordance the parameters including-system suitability, specificity, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length±2 nm). The method is validated according to ICH...
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Posted by admin on Apr 1, 2013 in |
In the present study, the in vitro antimicrobial and cytotoxic activity of root extract of Coccinia grandis were evaluated. Ethanolic crude extract with its various solvent soluble fractions of C. grandis showed significant antimicrobial activity by disc diffusion method on some gram positive and gram negative bacteria. The extracts exhibited 9-12 mm (approximately) of zone of inhibition comparing with standard ciprofloxacin (15-19 mm). In the brine shrimp lethality bioassay, the crude ethanolic extract showed significant cytotoxicity with LC50 of 2.496 mg/ml in comparison to vincristine sulphate (LC50 value 0.451 mg...
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