Posted by admin on Jun 1, 2013 in |
Three simple and sensitive and reproducible spectrophotometric methods were developed for the determination of spiramycin (SPM) in pure form and in pharmaceutical formulations (tablets). The first and second methods, A and B, are based on the formation of charge transfer complex between drug and the chromogenic reagents quinalizarin (method A) and alizarin red S (method B) producing charge transfer complexes in methanolic medium which showed an absorption maximum at 568 and 527 nm using methods A and B, respectively. Beer’s law was obeyed in the concentration range of 1.0–10 and 2.0-18 μg mL-1 with mean percentages accuracy of 100.39±0.89 and 100.26±0.60 using methods A and B, respectively. The third method C, is based on the reduction of Fe(III) by spiramycin in acid medium and subsequent interaction of Fe(II) with ferricyanide to form Prussian blue, which exhibits an absorption maximum at 760 nm. Beer’s law was obeyed in the concentration range of 2.0–12 μg mL-1 with mean percentage accuracy of 99.85±0.956. All variables were studied to optimize the reaction conditions...
Read More
Posted by admin on Jun 1, 2013 in |
The extracellular matrix (ECM) is the backbone in controlling cell behavior in the living organisms during the process of wound healing. The design of a suitable biomimetic nanofibrous scaffold which mimics the properties of natural ECM is a need of present time. In order to create a novel and unique wound dressing material, composite nanoscaled eudragit scaffolds loaded with gentamicin were prepared using the process of electrospinning. The nanofibers were characterized for size, size distribution, surface morphology and surface chemical structures using the Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). In-vitro release of gentamicin was also investigated. In vitro drug release tests confirmed that the nanofibers had pH-dependent drug release profiles. These properties indicate that the drug loaded eudragit nanofibrous scaffold has the potential to be used as a candidate in wound healing...
Read More
Posted by admin on Jun 1, 2013 in |
Reactive oxygen species (ROS) produced by activated macrophages play a major role in causing liver disorders. Small antioxidants and scavengers are protective only at very high concentrations and they poorly detoxify toxic ROS. Catalase (CAT) is an antioxidant enzyme that scavenges ROS and has great potential for treating liver disorders. In the present research work, gelatin nanoparticles bearing CAT were prepared using a two-step desolvation technique coupled with mannose and targeted in to liver to treat liver disorders associated with excess of ROS. It was characterized for shape, particle size, zeta potential, and percentage drug release and entrapment and confirmed by infrared spectroscopic studies. Experimental studies demonstrated that mannose Coupling enhanced their uptake into macrophages due to presence of mannose receptors in macrophages which increases their ability to scavenge ROS, produced by the activated...
Read More
Posted by admin on Jun 1, 2013 in |
As part of a research initiative to evaluate plants used for their nutritional and herbal values, the antimicrobial activity of Solanum Melongena’s fruit and Moringa oleifera’s leaveswere investigated. Each plant part was subjected to selective extraction using solvents of varying polarity: n-C6H14, CH2Cl2, EtOAc and CH3CH2OH. The n-C6H14 and CH3CH2OH extract of these two plants were tested for their antimicrobial activity at three different concentrations of 5%, 10% and 20% of crude extracts against three pathogenic bacterial strains: Eschericia coli, Staphyloccocus aureus and Klebsiella pneumoniae using the Disc diffusion assay. Both n-C6H14 and CH3CH2OH extracts showed antibacterial activity at a higher concentration of 20% of crude extract. The order of bacteria susceptibility to Moringa oleifera extract been S. aureus > K. pneumoniae > E. coli whereas that for Solanum Melongena extract been S. aureus > E. coli > K. pneumonia. The area of zone of inhibition ranges from 44.15 mm2 to 53.55 mm2. These investigations suggest that the extracts of Moringa oleifera and Solanum Melongena can be used as...
Read More
Posted by admin on Jun 1, 2013 in |
Antiretroviral therapy for decades is the appropriate treatment for infected populations with human immunodeficiency virus (HIV). However, it is associated with complications that affect the organism infected. The combination of antiretroviral therapy and pregnancy degrades iron metabolism. This work aims to evaluate and characterize the impact of the interaction between antiretroviral therapy and different stages of pregnancy in women of Abidjan on all biological indicators of iron status. The conducted investigations were a prospective, cross-sectional, descriptive analytical and case-control study. They concerned 275 women of reproductive age in all trimesters of pregnancy and in consultation to integrated center for bioclinical research of Abidjan (ICBRA). These women were divided into two groups: 135 pregnant women infected with HIV without antiretroviral therapy as controls and 140 pregnant women infected with HIV receiving antiretroviral therapy. All biological indicators of iron metabolism were determined either by colorimetric or immunoturbidimetric assays through blood samples from each pregnant woman or by calculation. The Student t test, factor analysis of variance (ANOVA) with two factors and...
Read More
