Posted by admin on Jan 1, 2013 in |
The present study was aimed at developing an oral floating system for Enalapril maleate with the objective to enhance the oral bioavailability of the drug. As it is a prodrug, oxidizing agent KMnO4 was used with distilled water and observed at 340 nm against a reagent blank, using PC Shimazdu UV Spectrophotometer. The obtained standard graph of drug was a straight line with coefficient correlation (R 2) = 9.9984. 12 formulations were prepared in 2 batches using varying concentration of hydrophilic swelling polymer HPMC K15 M and effervescent agent i.e. NaHCO3. Having a light sensitive drug all the experimental work had been done in the dark light room. Among the different formulation B1F6was considered as optimized formulation with a floating lag time of only 20 sec and floating time of more than 10 hour showed better floating behavior. It showed the release up to 60% of drug in 8 hr .The release of Enalapril maleate from all the formulations fitted to different release kinetic models, indicated that formulation B1F1...
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Posted by admin on Jan 1, 2013 in |
Staphylococcus aureus ATCC 6538 is an important strain of pharmaceutical and biotechnological industries. We studied the viability of culture suspension of Staphylococcus aureus strain ATCC 6538 at 2 to 8°C up to 360 days in 0.9% w/v NaCl. The culture suspension containing 10000 cfu/ml used in the study was stored at 2 to 8°C for 360 days in 0.9% w/v NaCl. Using 10 µl of above culture suspension, the viable count was made by the pour plate technique using Soyabean Casein Digest Agar medium in fixed interval of time during the 360 days storage period. During the storage period, population of Staphylococcus aureus ATCC 6538 strain decreased from 10000 cfu/ml to 9900 cfu/ml during the first 30 days, whereas the population decreased to 0 cfu/ml in 360 days. Findings emanate from the study indicates that 30 days storage period of Staphylococcus aureus strain ATCC 6538 at 2 to 8°C in 0.9% w/v NaCl is suitable for laboratories testing purposes on account of fact that in 30 days storage period,...
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Posted by admin on Jan 1, 2013 in |
Viruses are the major pathogenic agents that can cause variety of diseases in humans, animals and plants. Several deadly diseases like Hepatitis A, Hepatitis C, encephalitis, meningitis, and influenza are caused by viruses. Due to the constant and rapid emergence of viral pathogens, current recommended commercial drugs for above diseases are becoming resistant. In this view in present study forty three medicinal plants with numerous phytochemicals present in Dukes phytochemical database are subjected to PASS (Prediction of Activity Spectra for Substances) server for the prediction of their biological activity. Compounds with maximum probable activity for inhibiting specific viral targets are chosen and their drug likeliness is estimated based on Lipinski’s rule of five. The medicinal value of those compounds and its activity for inhibiting specific viral targets are analyzed from the PASS prediction results. Several interesting properties of Influenza virus, Arbovirus, Picornavirus, Flavivirus, and Herpes virus proteases with catalytic dyad and triad, active site, three dimensional structural features and their role in disease progression are review from the literature. ...
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Posted by admin on Jan 1, 2013 in |
Background: Oxystelma esculentum is used traditionally as diuretic, laxative, hepatoprotective and anti-ulcer. Objectives: The present work deals with the isolation and structure elucidation of Epicatechin from O. esculentum. Methods: Methanolic extract of the entire plant of O. esculentum was subjected to pilot TLC experiments and column chromatography. Results: This yielded a pure, white compound which resolved at Rf 0.3 on TLC plate using the mobile phase toluene: ethyl acetate (5:4) when developed in iodine chamber. This compound was subjected to UV, IR and 1H-NMR spectral analysis and its structure elucidation revealed it to be Epicatechin. Conclusion: This compound has been reported for the first time in this plant and can serve as a useful tool in its...
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Posted by admin on Jan 1, 2013 in |
Introduction: Solanum nigrum (S. nigrum) has been shown to be hepatoprotective by Ayurvedic physicians but has not been scientifically evaluated so far. So, the present study was undertaken to explore the hepatoprotective activity of S. nigrum against experimentally induced hepatotoxicity in albino rats. Aim of the study: To explore the hepatoprotective activity of S. nigrum. Materials and Methods: Albino Wistar rats weighing 150-200g of either sex were divided into six groups of six animals each. Group I was given normal saline (PO), group II Carbon tetrachloride (CCl4) (IP), group III Liv. 52 syrup for twenty days followed by carbon tetrachloride, group IV, V & VI received aqueous extract of Solanum nigrum ( 2ml/100g/day twice daily) orally for 10, 20 & 30 days respectively followed by CCl4. Blood was collected from anaesthetized animals & liver was dissected out. Alanine transaminase (ALT), alkaline phosphatase (ALP) & Total bilirubin were estimated and liver was subjected to histopathological examination. Results: ALT, ALP & Total bilirubin levels were significantly increased in CCl4 treated group...
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