Posted by admin on Oct 1, 2012 in |
This paper described validated high performance thin layer liquid chromatographic (HPTLC) method for estimation of Rosuvastatin Calcium (ROSU) and Aspirin (ASP) in capsule dosage form. The method involved separation of components by TLC on a precoated silica gel 60 F254 using a mixture of n-Hexane: Acetone: Ethyl acetate: Formic acid (6:3:1:0.2 v/v) as a mobile phase. Detection of spots was carried out at 240 nm for Rosuvastatin Calcium and Aspirin both. The mean retardation factor for Rosuvastatin Calcium and Aspirin were found to be 0.31±0.02 and 0.60±0.02, respectively. The linear regression data for the calibration plots showed good linear relationship with r2 value 0.995 and 0.995 in the concentration range of 500-1000 ng/spot and 3750-7500 ng/spot for Rosuvastatin calcium and Aspirin, respectively. The developed method was validated as per ICH Guidelines....
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Posted by admin on Oct 1, 2012 in |
The present work was undertaken to investigate various extracts of fruit of Wrightia tinctoria (F-Apocyanacea) for anti-diabetic activity in alloxan induced diabetic rats. A comparison was made between the action of extracts and known anti-diabetic drug glibenclamide (10mg/kg body weight). Oral administration of methanolic extract at a dose of 300 mg/kg/b. wt and ethyl acetate extract at a dose of 200 mg/kg/b. wt exhibited a significant (p<0.001, p<0.001) hypoglycemic activity in normal rats and significant (p<0.001, p<0.001) anti-hyperglycemic activity in alloxan induced diabetic rats respectively. The maximum reduction in blood glucose level was observed after 4 hours in case of methanolic and ethyl acetate extracts with a percentage protection of 37% and 42% respectively. In long term treatment of alloxan induced diabetic rats the degree of protection was determined by measuring blood glucose on 0, 1, 2, 4, 7, 14th day. Both the extracts showed a significant anti-diabetic activity comparable with that of glibenclamide. These results indicate that the W. tinctoria fruit extracts possess significant anti –diabetic...
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Posted by admin on Oct 1, 2012 in |
The study describes a validated reverse-phase HPLC method for the simultaneous estimation of paracetamol and naproxen in bulk and in tablet formulation. The proposed RP-HPLC method utilizes Eclipse XDB C18 column (150 ×4.6 mm i.d., 5 μm), optimum mobile phase consisted of gradient run of initial ratio of water (pH-2.5 adjusted with orthophosphoric acid): acetonitrile (87:13) with the effluent flow rate of 1.0 ml/min, and UV detection wavelength 263 nm. The developed method was statistically validated for linearity, precision, robustness, ruggedness and specificity. The method was linear over the range of 5-80 μg/ml for paracetamol and 3-48μg/ml for naproxen. The mean recovery was 99.72% and 100.88% for paracetamol and naproxen respectively. The intermediate precision data obtained under different experimental setup was quite concurrent with less critical % RSD. The proposed method can be useful in the quality control of paracetamol and naproxen in bulk drug and drug products....
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Posted by admin on Oct 1, 2012 in |
Excessive platelet activity is one of the most important factors responsible for the incidence of cardiovascular diseases and, also, play important role in coagulation cascade. In this study, the comparative effects of methanol extracts of three herbs on adhesion of the activated platelets to fibrinogen coated plates and clotting factors were investigated. Artemisia dracunculus, Punica granatum and Brberis vulgaris are used as blood anti-coagulatory plants in Iranian folk medicine. Platelets were prepared and incubated with different concentration of the test samples (50-200 μg/ml) for 60 min. The treated and un-treated platelets were then activated with thrombin (0.25U/ml) and their adhesions to fibrinogen coated plates were investigated. Based on obtained data, the methanol extract of Artemisia dracunculus, Brberis vulgaris and Punica granatum at a concentration of 200 μg /ml reduced platelet adhesion to coated wells by 35%, 25% and 20%, respectively. In addition, the effects of the crude extracts of each plant on atherogenic lipoproteins were also examined. The results indicated that the LDL and cholesterol concentration were dramatically reduced...
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Posted by admin on Oct 1, 2012 in |
A simple HPTLC method was used to determine the saponin profile of Barleria cristata L. crude leaf extract. The antimicrobial activity of saponin fraction from the leaves of Barleria cristata L. was studied in-vitro against four bacterial species and four fungal species by agar disc diffusion method. Klebsiella Pneumonia, Staphylococcus aureus, E. coli, Aspergillus parasites were the most inhibited microorganism. The present study suggests that the saponin fraction possess significant antimicrobial activity and can be used to develop a potential antimicrobial...
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