Posted by admin on Dec 1, 2012 in |
Cardiospermum halicacabum L. var. microcarpum (Kunth) Blume and var. luridum (Blume) Adelb. (Sapindaceae) are used for various ailments in Indian traditional medicines. The present study evaluated the antiulcerogenic property of seed oils (Petroleum ether extracts) in animal model. Ethanol induced gastric ulcers was used for this study and analysed for gastric volume, ulcer score pH, free and total acidity and sodium and potassium ions. Bio-chemical estimations like total proteins, total hexoses, hexosamine, fucose, sialic acid and protein were also made. Ulcer score was calculated for the same model. Oils were found to be more effective and exhibit concentration dependent anti-ulcer...
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Posted by admin on Dec 1, 2012 in |
A simple, precise and accurate UV-Spectrophotometric method has been developed and validated for estimation of Efavirenz in bulk and tablet dosage form. It shows maximum absorbance at 320 nm with methanol and water (30:70). Estimation was carried out by A(1%1cm) and by comparison with standard. Calibration graph was found to be linear (r2 =0.09657) over concentration range of 1-4μg/ml. The proposed methods appear to be simple, sensitive, and reproducible when checked for parameters like accuracy, precision, limit of detection for routine determination of Efavirenz in bulk as well as in tablet. The methods can be adopted in its routine...
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Posted by admin on Dec 1, 2012 in |
The main aim of our study is to evaluate the antiurolithiatic activity of pomegranate (punica granatum, Family: Puniaceae) and garlic (Allium sativum, Family: Liliaceae). For this, we have subjected the seeds of pomegranate and garlic bulbs to maceration using 95% of ethanol, ethyl acetate, petroleum ether and water at a ratio of 1:4 for 24 hours, followed by reflux over a water bath for 3 hours and the obtained extract was concentrated under vacuum. The prepared extracts were then subjected to titrimetry and colorimetry for the estimation of calcium and phosphate respectively. By comparing the result of different extracts, we have found that ethanol extract of garlic has showed better result in inhibiting precipitation of calcium and phosphate. But individual isolation and further studies are to be carried out to confirm this...
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Posted by admin on Dec 1, 2012 in |
The objective of the present study was to investigate the antioxidant and analgesic activity of ethanol extract of Xanthium indicum Koen. Family: Compositae. Ethanol extract of X. indicum plant was prepared and tested for its phytochemical and pharmacological investigation. Preliminary phytochemical investigation showed that extracts possessed the active principles-alkaloid, carbohydrate, glycoside, flavonoid & tannin. The ethanol extract of X. indicum showed significant antioxidant property in the qualitative assay. The extract showed the antioxidant property by the presence of strong yellow spot on a purple background on the TLC plate & it also in the quantitative assay, X. indicum extract displayed free radical scavenging activity in the DPPH assay (IC50 ~141.25 µg/mL) which is comparable to standard (ascorbic acid, IC50 ~14.12 µg/mL). The extract showed dose-dependent reduction ability (Fe3+ to Fe2+ transformation) in reducing power assay; showing a maximum absorbance of 0.492 at a concentration of 100 μg/mL of the ethanol extract comparable to that of ascorbic acid which were used as positive control and gave maximum absorbance of 0.659...
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Posted by admin on Dec 1, 2012 in |
Nosocomial infections even in this modern era antibiotics, continue to remain an important and formidable consequence of hospitalization. Our aim of the study was to investigate the antimicrobial susceptibility against hospital-associated infecting microorganisms. 30 Clinical samples were taken from OPD of GMC Hospital, Bhopal (MP), India. Among 30 clinical isolates we identified bacterial pathogens as Staphylococcus aureus (10), Escherichia coli (10) and Pseudomonas aeruginosa (8). Antimicrobial susceptibility assay was performed by the reference criteria of clinical and laboratory standard institute guidelines. In the present study antibiotic susceptibility results showed that all 10 (100%) S. aureus isolates were resistant to penicillin, 8 (80%) S. aureus isolates were resistant to cefuroxime, 7 (70%) S .aureus isolates were resistant to ciprofloxacin, 5 (50%) S. aureus isolates were resistant to amikacin, 2 (20%) S. aureus isolates were resistant to nitrofurantoin whereas, 0 (0%) S. aureus isolates were found to be resistant to cefazolin. For E. coli isolates all 10 (100%) were resistant to cefuroxime, 7 (70%) E. coli isolates were resistant to amikacin,...
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