Posted by admin on Sep 1, 2012 in |
This study was to evaluate the antioxidant and antibacterial efficacy of herbal crude extract of Murraya koenigii againsthuman Cariogenic pathogens. In this study Methanolic extract of Murraya koenigii were studied for phytochemical composition and GC-MS for active compound. Antioxidant potential assay by 1,1-Diphenyl- 2-picrylhydrazyl (DPPH), 2, 2’-azinobis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays. Further extracts were studied for antibacterial activity. Methanolic extract of Murraya koenigii revealed the presence of terpenoids, alkaloids, tannins, steroids, and saponins. Reducing ability of extracts was in the range (in μm Fe(II)/g) of 3176.98±63.87 were closed to quercetin 3208.27 ± 31.29. A significant inhibitory effect of extract (IC50 = 0.019 ± 0.69 mg/ml) on ABTS free radicals was detected. The antioxidant activity of the extract (96%) DPPH radicals were comparable with that of ascorbic acid (98.8%) and BHT (97.5%). Antibacterial effect of 50% and 10% methanolic extracts was detected on Cariogenic pathogens as compared with standard drug Amoxicillin. GC-MS results revealed the presence of hexadecanoic acid, linoleic acid, oleic acid, propanoic...
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Posted by admin on Sep 1, 2012 in |
Ropinirole hydrochloride is indicated in Parkinson’s disease and Restless leg syndrome. In advanced Parkinson’s disease the usual dose of Ropinirole hydrochloride is 0.25 to 5 mg three to four times a day. Hence, an attempt was made to develop a once-a-day controlled release Osmotic drug delivery system. This may offer significant patient benefits by providing enhanced efficacy and reduced side effects and may also reduce the number of daily doses compared to conventional therapies. An oral push-pull system that can deliver Ropinirole hydrochloride for extended period of time has been developed and characterized. A bilayer osmotic drug delivery system was developed using drilling technique. The push layer swells releasing the drug at a controlled rate. An optimized system was selected to study the effect of pH of dissolution medium and the effect of agitation intensity. The drug release was found to follow zero order kinetics. The developed push-pull osmotic system showed the desired once-a-day release...
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Posted by admin on Sep 1, 2012 in |
Background: Noise has been recognized as one of the risk factors for cardiovascular diseases. Garlic is one of the oldest medications used in human culture. Garlic is used in herbal medicine for thousands of years and also as one of the main food ingredient. The present study was aimed to investigate the cardioprotective effect of garlic after noise exposure in Wistar albino rats. Methods: Animals were exposed to 1 day, 15 days and 30 days of noise stress exposure. The myocardial activity was evaluated by estimating the heart mitochondrial enzymes like malate dehydrogenase, isocitrate dehydrogenase, alpha ketoglutarate dehydrogenase, NADH dehydrogenase and cytochrome C oxidase. Garlic was administered to the animals at the dosage of 125 mg/kg b.w.) Conclusion: The result indicated that noise exposure affects the mitochondrial performance in the myocardium and that long-term consumption of garlic is beneficial. As garlic is already under human consumption, dietary supplementation of garlic for prolonged period may be beneficial as one could not avoid noise in the modern way of living. Results: Exposure...
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Posted by admin on Sep 1, 2012 in |
The purpose of this work was to develop and characterize a vesicular drug carrier for topical delivery of Quetiapine to overcome the problems related with oral route that is high first pass metabolism and fluctuating drug plasma concentration. The effects of key formulation variables on entrapment efficiency (EE %), vesicle size and in vitro drug permeation were studied using a Box- Behnken design. Liposomes bearing Quetiapine were prepared by using saturated lipids like 1, 2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1, 2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) with relatively less stability problems through rotary evaporation method. The liposomal formulation was characterized for various parameters including EE %, vesicles shape, size distribution, lamellarity, in vitro release study, skin permeation and stability studies. Firstly liposomal suspension was prepared and then previously prepared suspension was incorporated in carbopol 940P gel with an objective of enhancing stability of liposome by avoiding aggregation of vesicles and for better skin permeation. The encapsulation efficiency of drug was found to be ranging from 60.59±4.54% to 83.56±2.97%. Nano liposomes were found to have mean...
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Posted by admin on Sep 1, 2012 in |
Cyclin dependent kinases (CDKs) are known as cell cycle regulators in eukaryotic cell cycle. Different CDKs (CDK2, CDK4 etc.) are having structure homology among them. Using computer based molecular modeling tools, interactions between naturally occurring terpene based compounds with crystal structure of CDK4 mimic CDK2 enzyme having PDB ID : 1GII. Using In-silico techniques, the binding energies between terpene based compounds and receptor enzymes are calculated in the form of ΔG in kcal/mol. The reported binding energies for series of molecules are ranging from –5.35 to –13.20 kcal/mol. The negative docking energies and a few hydrogen bonds between selected ligands and receptor enzyme support the affinity of Terpene based compounds with CDK4 mimic CDK2 enzymes. It is also found out that those compounds having carbon atoms 30-31 interacts better with enzyme, whereas larger size compounds having carbon atoms higher than 40 show weak interactions. It is concluded that Tri-terpene class of compounds are the best CDK4 mimic CDK2...
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