Posted by admin on Sep 1, 2012 in |
Oxidative stress has been implicated in the eiteology of diabetic neuropathy (DN) and anti-oxidants have shown to ameliorate this condition. Previous studies have indicated that early treatment with vitamin E acetate (VE) and methylcobalamin (MCA) attenuated surgery induced neuropathy in rats. The aim of this study was to test the effect of early co-administration of VE (50 mg/kg) and MCA (500µg/kg) (VEM) on the development of DN in laboratory rats. Male Wistar rats were divided into the three treatment groups, sham (non-diabetic, vehicle treated), diabetic rats (vehicle treated; (DU), and diabetic rats which received VEM treatment (DVEM). Diabetes was induced by single injection of alloxan monohydrate (120 mg/kg). Body weights of all subjects were noted daily and serum glucose levels (SGL) were measured at regular intervals. Behavioural assessment of thermal hyperalgesia (TH) was performed using the hot plate test and warm water tail immersion test. Finally, motor nerve conduction velocities (MNCV) were measured on day 45. Significant enhancements in SGL were noted for DU rats and this remained throughout...
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Posted by admin on Sep 1, 2012 in |
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-diabetic drugs Metformin hydrochloride (MT), Rosiglitazone (RT) and Sitagliptin (ST). The separation was achieved on ACE 3 150 mm X 4.6mm, 3.5µm column using gradient method. The mobile phase at a flow rate of 1.5 mL min−1 consisted of 10 mM sodium hexane sulphonate monohydrate and 10 mM Potassium dihydrogen phosphate buffer with acetonitrile and methanol in Gradient ratio. The UV detection was carried out at 210 nm. The forced degradation for these drug substances were performed under Acid, Base, Oxidative, Photolytic and Thermal stress conditions. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage...
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Posted by admin on Sep 1, 2012 in |
The ethanolic extracts of aerial part of Ocimum canum (OC) were studied for its antioxidant activity and its role to prevent renal ischemia. The in vitro antioxidant models used were DPPH radical scavenging activity, Hydroxyl peroxide radical scavenging method, reducing power assay & FRAP assay. The study was carried out at different concentration (250, 500, 1000, 2000 µg/ml). Further, the antioxidant activity was studied by using in vivo method to prove its potency in preventing ischemia by incorporating renal ischemia/reperfusion model in Wistar albino rats. The animals were divided into four different groups of six rats in each group. Group-1 was served as Control and received oral saline only once daily for 28 days. Group-2 received (oral saline + RI). Group 3 received Ocimum canum ethanolic leaf extract 300mg/kg bwt dose orally for 28 days, Group-4 were pretreated with Ocimum canum ethanolic leaf extract an oral dose of 100mg/kg bwt for 28 days. After the experimental period all rats were sacrificed and antioxidant defense system and oxidative stress in...
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Posted by admin on Sep 1, 2012 in |
In quest of better anti-amoebic agents, quantitative structure-activity relationship (QSAR) studies were performed on a series of pyrazoline & dioxazoles derivatives with the help of PM5 calculations and geometry optimizations using CAChe software. Multiple Linear Regression (MLR) analysis was performed to derive QSAR models using the descriptors, molecular weight (MW), conformation minimum energy (ɛ), HOMO energy (eHOMO), shape index, basic kappa second order (k2), absolute hardness (h), electronegativity (c), electrophilicity index (ω), molar volume (MV), molar refractivity (MR), LogP (LP), parachor (Pc) and solvent accessibility surface area (SASA). The QSAR models equations of anti-amoebic agents have been developed by using maximum of seven descriptors, in which conformation minimum energy, shape index, molar volume and parchor were present have good predictive powers of correlation coefficients. These models can successfully predict the anti-amoebic activity of any newly discovered pyrazoline and dioxazole derivatives which can later be tested in...
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Posted by admin on Aug 31, 2012 in |
Retraction Note : Shokri N, Azizi E, Akbari Javar H, Fouladdel Sh, Khalaj A, Dinarvand R. In vitro and in vivo evaluation of poly (caprolactone fumarate) nanoparticles. International Journal of Pharmaceutical Sciences and Research. 2012; 3(9):3106-3115. It has come to the attention of the IJPSR Editors that there are substantial overlaps between the text of the above article and the following papers. It has been pointed out that the above study appeared to contain a high level of similarity with two articles by the same authors published as below: Shokri N, Akbari Javar H, Fouladdel Sh, Khalaj A, Khoshayand M, Dinarvand R, Atyabi F, Nomani A, Azizi E. Preparation and evaluation of poly (caprolactone fumarate) nanoparticles containing doxorubicin HCI. Daru. 2011;19(1):12-22. PMID: 22615635. and Shokri N, Akbari Javar H, Fouladdel Sh, Khalaj A, Dinarvand R, Azizi E. Preparation and characterization of crosslinked and non-crosslinked polycaprolactone fumarate (PCLF) NPs as carriers for doxorubicin HCl. Afr J Pharm Pharmacol. 2011;5(7):797-805. Following an internal investigation, it was established that the studies were...
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