Posted by admin on Oct 1, 2012 in |
In the present work for the simultaneous estimation of Metolazone (METO) and Spironolactone (SPI) Ratio Derivative Spectrophotometric method have been developed. In this method the overlapping spectra of Metolazone and Spironolactone were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. Methanol:water (1:4) was used as solvent for the method. Amplitude at 241.0nm and 263.0 nm were selected in the ratio derivative spectra to determine METO and SPI, respectively. Beer’s law was obeyed in the concentration ranges of 1-5μg/mL and 5-25 μg/ mL METO and SPI, respectively in the method. The % assay for commercial formulation was found to be in the range 98.00% – 102.00 % for METO and 99.00% – 102.60 % for SPI by the proposed method. Recovery was found in the range of 99.74 – 100.76 % for METO and SPI in the Formulations. The results of analysis have been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods which were carried out...
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Posted by admin on Oct 1, 2012 in |
OBJECTIVE: To evaluate the anti-inflammatory activity of indomethacin in different experimental anti-inflammatory animal models METHODS: Swiss albino rats weighing around 150 g – 250 g of either sex were randomly selected and divided into 6 groups (n=6) for 3 models, each having control and Standard . Materials used are Carrageenan, turpentine, Cotton pellets, Mercury and water plethysmographs. Methods adopted in this study are; (1) Carrageenan induced rat-paw edema (paw volume measured through mercury and water plethysmographs), (2) turpentine induced arthritis (by measuring lateral diameter of knee joint), as models of acute inflammation, (3) Cotton pellet induced granuloma (measured through dry weight of granulation tissue) as model of sub-acute inflammation. RESULTS: The following observations were made. Indomethacin exhibited potent anti-inflammatory activity in Carrageenan induced edema model (83.34% inhibition in mercury plethysmograph) compared to control. But its anti-inflammatory activity is of lower order both in turpentine induced arthritis & cotton pellet granuloma model (48% & 40.16% inhibition respectively). All the results obtained were statistically significant with P< 0.001 in all four...
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Posted by admin on Oct 1, 2012 in |
The history of herbal medicines is as old as civilization. For this purpose of ascertaining the phytochemical constituents, the present work was performed into the phytochemical investigation of the plant Avicenna alba. Separation and isolation of the constituents of interest, characterization of isolated compounds and investigation of the biosynthetic pathways of the particular pathway and quantitative evaluation. Another reason for choosing this plant is the availability of the plant. Avicenna alba is the important plant used from the past. It was reported to show antimicrobial activity in literature survey. So now, the time has come to perform a proper phytochemical investigation to find its proper way into medicine. Our plan of work is to identify and isolate the chemical constituents of the aerial part of Avicennia alba and to evaluate its pharmacological action. With the above aim, this experimental work was...
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Posted by admin on Oct 1, 2012 in |
The use of bronchodilators has been increasing for the past few decades. The literatures have reported a high rate of adverse events. It has also been found out that beta-agonists are associated with an increased morbidity and mortality rate. Respiratory tolerance develops due to regular use of beta-agonists, which are widely prescribed in asthma and bronchiolitis also is of major concern. Objective: To evaluate the rational use of bronchodilator drugs by analyzing the appropriateness of the prescription. Methodology: Prospective observational study. Study site: A 500-bedded Multispecialty Tertiary care Teaching Hospital. Result: In the study population of 133 patients male children were found to be more (58.6 %) than the female children (41.4%) Most of the bronchodilators were prescribed for infants (48.1%). Of the 133 pediatric patients, the major prescription was for SABA (99.2%). Of which the major prescription was for salbutamol (63.2%). Most of the Children with asthma were prescribed with combination therapy of salbutamol and ipratropium (81.3%). In WALRI, patients were prescribed with salbutamol (40.4%) and in acute...
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Posted by admin on Oct 1, 2012 in |
In the present study, natural gums (Hibiscus rosa – sinensis mucilage and Modified gum karaya) was investigated as superdisintegrants for use in fast dissolving tablet formulations containing amlodipine besylate. Fast dissolving tablets of amlodipine besylate was formulated using different concentrations (2, 4, 6, 8, 10) of natural superdisintegrant viz; Hibiscus Rosa – sinensis mucilage and modified gum karaya. Prepared tablets were evaluated for thickness, hardness, friability, uniformity of weight, disintegration time, wetting time and dissolution study. The formulated tablets had good appearance and better drug release properties. The best formulation of Hibiscus rosa – sinensis mucilage (FHR5) and modified gum karaya (FMGK5) shows shorter disintegration time and shows 100% release is selected as optimized formulation. Hence, the present study revealed that this natural superdisintegrants (Hibiscus rosa – sinensis mucilage and modified gum karaya) showed better disintegrating property than the most widely used synthetic superdisintegrants. Studies showed that naturally obtained polymers are more beneficial without any side effects. Optimized formulation was subjected to stability studies as per ICH guidelines and...
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