Posted by admin on Aug 1, 2012 in |
The aim of the present work is to develop simple accurate, precise and cost effective UV-Vis spectrophotometric method for the estimation of pyrimethamine in bulk and pharmaceutical dosage form and determination of percent degradation by using different stress condition. The solvent used was methanol and distilled water (40:60) and λmax of the drug was found to be 272.5nm. A linear response was observed in the range 2-20 µg/ml with a regression coefficient 0.999. The method was then validated for different parameters as per the ICH guidelines. This method can be used for the determination of pyrimethamine in quality control of formulation without interference of the excipients. Pyrimethamine was subjected to stress degradation under different conditions recommended by ICH. The samples generated were used for degradation studies using the developed...
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Posted by admin on Aug 1, 2012 in |
Indigofera linifolia Retz(Fabaceae) is herb which spread throughout the India and found to be used by the tribal to cure inflammations. Based upon the review the present study focused on screening of Ant-inflammatory and Anti-bacterial activity of Indigofera linifolia Retz. The coarse powder of whole plant was extracted by cold maceration method using various solvents successively. Preliminary phytochemical screening was carried out to confirm the presence of phytoconstituents. Based upon the phytochemical tests chloroform and ethanol extracts (200mg/kg) were taken for anti-inflammatory and antibacterial activity screening. Anti inflammatory studies was carried out by Carrageenan induced paw edema method in Wistar albino rats. Anti bacterial screening was carried out in chloroform and ethanolic extract by disc diffusion method in three different concentrations (100μg, 150μg, 200μg /disc) and the diameter of the zone of inhibition were recorded. The percentage increase in paw edema was reduced in both chloroform (18.20±1.32) and ethanol extract (24.22±1.12) treated animals when compared with standard Indomethacin (19.18±1.02) and control (40.10±1.22) animals. The chloroform extract possessed significant anti...
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Posted by admin on Aug 1, 2012 in |
The voltammetric behavior of olopatadine was investigated at hanging mercury drop electrode using cyclic voltammetry and differential pulse voltammetry. The drug under study exhibited a single, well-defined reduction peak owing to the reduction of carbon-carbon double bond. The electrode and reaction conditions which yielded maximum peak current were established using differential pulse voltammetry. A linear relationship was observed between the peak current and the concentration of olopatadine over the range 1.0 x10-8 M to 4.0 x10-7 M. The limits of detection and limits of quantitation were found to be 1.92ng/mL and 6.34ng/mL respectively. The proposed method was successfully applied for the determination of olopatadine in pharmaceutical formulations without interference from...
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Posted by admin on Aug 1, 2012 in |
Phytochemicals are the bioactive components present at micro level in our daily diet, especially the phenolic antioxidants, contribute to the prevention of cardiovascular and other degenerative diseases. Moringa oleifera (MO) leaves polyphenols effect on the activity of 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase was studied using a spectrophotometric assay based on the stoichiometric formation of coenzyme A (SH) during the reduction of microsomal HMG CoA to mevalonate at different concentrations (200- 400 µg). Activity of HMG- CoA reductase was inhibited by MO polyphenols significantly (p<0.05) at the concentrations studied. The Coenzyme A released at the end of assay in the control was higher (18.87±0.12 n Moles) significantly than the MO polyphenol extract, which was dose dependent (14.69±0.07 to 10.26±0.33 n Moles). The results reveal the potency of the MO polyphenols in inhibiting the HMG- CoA reductase. Hence, the MO polyphenols can be explored for its role as a cholesterol lowering...
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Posted by admin on Aug 1, 2012 in |
The aim of the present study was to investigate the applicability of liquisolid technique in improving the dissolution properties of Valsartan in a solid dosage form. This study was designed to optimize and evaluate the effects of different formulation variables: amount of liquid vehicle (X1), ratio of carrier to coating material(X2) and amount of magnesium oxide(X3) on angle of repose (Y1), hardness(Y2) and in-vitro release(Y3) of formulation using three level three factor Box-Behnken statistical design. The non-linear quadratic model generated by the design is of the form: Y = A0 + A1X1 + A2X2 + A3X3 + A4X1X2 + A5X2X3 + A6X1X3 + A7X12 + A8X22 +A9X32 + E, where Y is the measured response associated with each factor level combination. Contour and response surface plots were depicted based on the equation given by the model. The optimization procedure generated the maximum overall desirability value. The optimized formula yields observed values close to the predicted values. Furthermore, the commonly used carrier and coating materials in liquisolid systems Avicel® and...
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