Posted by admin on Jun 1, 2012 in |
Most of the therapeutic agents are recommended through an oral route, but oral route has disadvantages like first pass metabolism, liver toxicity, etc, due to gastrointestinal pH. This leads poor bioavailability of drugs, which are not stable in G.I pH. To overcome this problem, increase the bioiavailibility, reduce the dose and dose dumping Transdermal delivery system is better option as novel drug delivery system, which bypass the hepatic first pass metabolism, and avoid drug degradation due to systemic absorption of the drug. Minimize plasma level fluctuations and extend the drug activity besides improving patient compliance. Sumatriptan succinate is a selective 5-hydroxytryptamine receptor subtype agonist. Sumatriptan succinate is chemically designated as 3-[2-(dimethylamino)ethyl]-N-methyl-indole-5-methanesulfonamide succinate. Sumatriptan succinate is a white to off-white powder that is readily soluble in water. Oral administration of Sumatriptan succinate suffers from poorbioavailability, partly due to presystemic metabolism- some of it gets broken down in the stomach and bloodstream before it reaches the target arteries. Sumatriptan is metabolized primarily by monoamine oxidase A into an indole acetic acid...
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Posted by admin on Jun 1, 2012 in |
The cytotoxic effect of crude extracts (n-hehane, ethyl acetate and chloroform soluble fractions) of root of Acacia nilotica was observed by brine shrimp lethality bioassay. The LC50 values of n-hexane, ethyl acetate and chloroform soluble fraction found to be 1.956, 2.079 and 1.981 respectively indicating that the some of the compounds of the extract are biologically active. From this experiment, it was revelead that the test sample showed different response at different concentrations. The mortality rate of brine shrimp was found to be increased with the increased concentrations of sample, and a plot of log of concentration versus percent mortality on the graph produced an approximate linear...
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Posted by admin on Jun 1, 2012 in |
Analytical monitoring of Pharmaceutical products is necessary to ensure its safety and efficacy throughout all phases of the drug. A systematic approach should be adapted to the presentation and evaluation of stable information, which should include, as necessary, physical, chemical, biological and microbiological test characteristics. Indian System of Medicine (ISM) frequently uses metal/mineral drugs. It is mandatory to standardize the preparatory procedures. For the Pharmaceutical standardization, three batches of the Panchavaktra ras were prepared and analytical study of final product carried out. Panchavaktra ras consist equal parts of Parada (Mercury), Gandhaka (Sulphur), Tankana (Borax) Pippali (Piper longum L.) and Marica (Piper nigrum L.), and Mardhana (Grinding) was done carefully with sufficient quantity of juice of Datura metel leaves for 24 hours and a final product was obtained in the form of Vati (tablet) form. It is one of the formulations mentioned in Amavata (Rheumatoid Arthritis) disease. The final products were subjected to Powder X-Ray Diffraction (XRD) studies and values of XRD peaks of particular Panchavaktra ras were observed. This...
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Posted by admin on Jun 1, 2012 in |
Pseudomonas aeruginosa, is one among the few drug resistant organisms causing Urinary Tract Infection. Most strains of Pseudomonas aeruginosa are significantly more resistant, even in the absence of R plasmids, to many antimicrobial agents, including β-lactams, tetracycline, chloramphenicol, and fluoroquinolones, than most other gram-negative rods. This broad-range resistance has so far been assumed to be mainly due to the low permeability of the P. aeruginosa outer membrane. Mex-proteins are the multidrug exporter proteins that are significant in drug ejection, which is the main reason for drug resistance. This limits the therapeutic options. Inhibition of multi drug resistant efflux pumps can restore the activities of antimicrobial agents that are substrates for these proteins. Hence efforts are now taken to screen few medicinal plants, which are both economic and less toxic. Among the several plants screened, we have chosen the acetone extract of Elephantopus scaber from which we purified a new terpenoid for our study. Its structure was generated using CHEMSKETCH software. The novel terpenoid was evaluated as potent efflux inhibitor. In...
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Posted by admin on Jun 1, 2012 in |
In this study, isolation, screening, characterization, antibiotic susceptibility tests, curing & Determination of B-complex vitamin production by potent probiotic Lactobacillus strains from different sources of meat was analysed. 250 grams of intestine parts of goat meat and beef each were transferred to MRS broth to screen the potent probiotic Lactobacillus strains. Initially 57 strains were isolated and confirmed by gram`s staining and catalase tests. After that, 51 strains selected for further biochemical tests to confirm all were Lactobacillus. Later, 41 strains were selected for antibiotic susceptibility tests by using three different classes of antibiotics viz., tetracycline, ampicilin, and streptomycin. Those strains which showed resistant to all three antibiotics used, further selected for curing of plasmid DNA and again antibiotic susceptibility tests was performed by the same antibiotics used before. Finally, only 5 strains were selected as potent probiotic Lactobacillus and unfortunately all strains from Beef intestine derived. Then, determination of B-Complex vitamin production of those selected 5 potent probiotic Lactobacillus by thin layer chromatographic method; Spectroflurophotometric method and RP-HPLC...
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