Posted by admin on Jan 1, 2012 in |
Valsartan (VAL) and Hydrochlorothiazide (HTZ) are used in combination in treatment of Hypertension. Two simple, accurate, precise, economical and reproducible UV spectrophotometric methods have been developed for the estimation of Valsartan and Hydrochlorothiazide in Pharmaceutical formulation. Method I- Absorption ratio method (Q-analysis) using two wavelengths, 265nm (isobestic point at which both the drugs exhibit absorbance) 249nm (λmax of Valsartan) and Method II- Area under Curve method. For the second method Area under the Curve in the range of 249 -259nm and 261-281nm was selected for the analysis of Valsartan and Hydrochlorothiazide respectively. Linearity for detector response was observed in the concentration range of 2-24mg/ml & 2-14mg/ml for Valsartan and Hydrochlorothiazide respectively. The results of analysis have been validated statistically and by recovery studies the value of standard deviation was satisfactory and recovery studies ranging from 99.54 – 99.97 % for Valsartan and 99.75 – 101.04 % for Hydrochlorothiazide were indicative of the accuracy and precision of the proposed method The proposed methods were successfully applied for the determination of...
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Posted by admin on Jan 1, 2012 in |
Diabetes mellitus is a chronic metabolic disorder associated with hyperglycemia, oxidative stress and dyslipidemia. Hemidesmus indicus is employed as an indigenous medicine for a variety of ailments from earlier days. The present study was aimed to evaluate the role of Hemidesmus indicus in alloxan-induced experimental diabetic rats. The effect of oral administration of Hemidesmus indicus root extract (400 mg/kg b.w.) on glucose tolerance, the levels of blood glucose, hemoglobin, glycosylated hemoglobin, plasma insulin, protein, lipid peroxides, enzymatic and non-enzymatic antioxidants, lipid profile, muscle glycogen content were determined in control and experimental groups of rats. The altered levels of blood glucose, hemoglobin, glycosylated hemoglobin, plasma insulin, and protein in the diabetic rats were significantly reverted back to near basal values by the administration of ethanol extract of Hemidesmus indicus root to diabetic rats for 30 days. The levels of lipid peroxides in the plasma and pancreatic tissues of diabetic rats were elevated significantly and were normalized by the administration of Hemidesmus indicus root extract. The activities of pancreatic enzymic antioxidants...
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Posted by admin on Jan 1, 2012 in |
Turnera ulmifolia Linn. belonging to family Turneraceae was investigated to evaluate in-vitro antibacterial activity of ethanol extract of the leaves against gram negative bacteria such as Salmonella typhi, Pseudomonas fluorescens, Pseudomonas aeruginosa and Escherichia coli. The pathogens were tested by disc diffusion assay method and minimum inhibitory concentration was evaluated. An attempt has been made to compare the activity of extract with standard ciprofloxin. The pathogens were used in the study was showed potential activity of the ethanolic extract of the plant. Maximum activity was seen in case of Pseudomonas fluorescens where the zone diameter was 32 mm...
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Posted by admin on Jan 1, 2012 in |
The objective of the present investigation was to study the effect of critical formulation parameters affecting release of isoxsuprine hydrochloride from matrix tablets using combination of polyethylene oxide (PEO) and dicalcium phosphate (DCP). The powder blend consisting of drug and excipients was analyzed for angle of repose, Carr’s index and Hausner’s ratio. The tablets were prepared by direct compression method. To assess the compressional behavior of the drug-excipient blend, the tablets were analyzed for friability and crushing strength. The in vitro drug release study was carried out in distilled water. The powder blend exhibited satisfactorily flow as measured by angle of repose, Carr’s index and Hausner’s ratio. The formulation ingredients showed satisfactory tableting properties (friability <1%, crushing strength ≥ 4 kgf). The drug release was modified on addition of PEO and DCP. Addition of 5 to 25% DCP in the formulation of matrix tablets caused apparent difference in the drug dissolution in distilled water. However, the difference was insignificant as analyzed by analysis of variance (ANOVA) and similarity factor...
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Posted by admin on Jan 1, 2012 in |
In order to explore the antihistaminic and anthelmintic activity associated with piperazine framework, several 1-benzhydryl piperazine derivatives and 1- benzhydryl piperazine acyl derivatives were synthesized. Their chemical structure was characterized and confirmed by H1 nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR). The target compounds were synthesized by the nucleophilic substitution reaction of 1-benzhydryl piperazine with various acyl chlorides. The intermediate (AT-1) was prepared by reaction of benzhydryl chloride and piperazine in presence of anhydrous potassium carbonate in N-N dimethylformamide. Further 1-benzhydrylpiperazine was reacted with various acyl chloride in presence of triethylamine in dichloromethane afforded target compounds (AT5 -9). The anthelmintic activity of compounds were evaluated by Garg and Atal method while antihistaminic activity was evaluated by in- vitro guinea pig...
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