Posted by admin on Jan 1, 2012 in |
This paper describes the study to measure the sensitivity and accuracy of two techniques, chromatography and spectrophotometry for the direct determination of citalopram in pharmaceutical formulations. Chromatography was performed on SE-30 column (1 m × 3 mm i.d) using Nitrogen as a carrier gas (flow rate of 40 ml/min), having retention time of 12.8 min. Calibration curve was found linear in the range of 200-400 µg/ml. LOD and LOQ values were found as 1.1726 µg/ml and 3.5535 µg/ml respectively and the recoveries were in the range of 98.36–100.07%. In...
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Posted by admin on Jan 1, 2012 in |
Most of the traditional systems of medicine are effective but they lack of standardization. So there is need to develop standardization technique. Central Council for Research in Ayurveda has given preliminary guidelines for standardization the conventionally used formulations. Standardization of herbal formulations is essential in order to assess the quality of drugs, based on the concentration of their active principles, physical and chemical standards. This article reports on standardization of Yakrita Plihantak Churan a polyherbal Ayurvedic formulation used as hepatoprotective. Samples were collected from local market and were subjected standardized on the basis of organoleptic properties, physical characteristics, and physic-chemical evaluation. Microscopic characterization was compared with authentic ingredients as a reference. It was observed that commercial sample from market matched exactly with that of authentic standards after performing the standardization. The set parameters were found to be sufficient to evaluate the studied churn can be used as reference standard for the quality control/assurance...
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Posted by admin on Jan 1, 2012 in |
Fruits of the plant were air dried for several days and cut into small pieces, which were subjected to oven dried for 24 hours at 45-50°C to effect further chopping. Cold methanol extract of fruits were subjected to acid-base treatment to separate the alkaloids. The treated extract was subjected to column chromatography to isolate the pure compound. The xanthine alkaloid, 1, 3, 7-trimethyl-1H-purine-2, 6 (3H, 7H)-dione, caffeine (compound 1) and also p-anisaldehyde (compound 2) were isolated from the column fraction of this ‘alkaloid containing residue’ by elution with ethyl acetate/10% methanol and ethyl acetate/50-100% methanol, respectively. The isolated pure compounds were identified by extensive spectral (1H-NMR, 13C-NMR, DEPT, HMBC, HSQC, Mass) data analysis. Greshoff isolated an alkaloid, pithecolobin from the bark, which is saponin like in its action. Wehmer stated that the seeds also contain pithecolobin. In the Philippines, a decoction of the inner bark (fresh cambium) and the fresh leaves of this tree is used in diarrhea. All these above mentioned study lead us to work on...
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Posted by admin on Jan 1, 2012 in |
Schiff Base derivatives are important class of compounds. They possess different types of Biological activities like antibacterial, antiviral, anti HIV, antifungal etc. Schiff base derivatives are prepared by the condensation of aldehyde and amine and these compounds are characterized by chemical and instrumental methods. Their important biological properties have been...
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Posted by admin on Jan 1, 2012 in |
CLX (celecoxib) is a highly hydrophobic non-steroidal anti-inflammatory drug with high plasma protein binding. We describe here the encapsulation of CLX in MLVs composed of SPC and variable amounts of cholesterol. The influence of drug – lipid ratio was studied and amount of the drug could be encapsulated was optimized. The effect of cholesterol and other process parameters were studied to obtain the liposomal vesicles with desired quality. All the prepared formulations were characterized for their physico chemical properties such as appearance, vesicle size, vesicle size distribution and percentage drug entrapment. Stability of the liposomes in terms of their drug leakage and drug retention behaviour was studied by storing the liposomal formulations under different conditions for the period of 30 days. The optimized formulation parameters and process parameters resulted the liposomes with mean vesicle diameter of 4.81μ. The maximum percentage drug entrapment was achieved with the formulation CL3 which contains the drug – lipid ratio of 1:10%W/W and the percentage drug entrapment is equal to 72.33±0.64 (%). In vitro...
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