Posted by admin on Dec 1, 2011 in |
The essential oil of the aerial parts of Launaea nudicaulis was isolated using Clevenger’s apparatus and analyzed by GC/MS instrument. Different extracts of the aerial parts of Launaea nudicaulis were tested for their activity against Staphylococcus aureus and Escherichia coli. Seventeen constituents were identified in the essential oil. The major constituents in the essential oil are limonene, Z-citral and E-citral. The three polar extracts (hydro alcoholic, Ethyl acetate and butanol extracts) showed good activity against Gram +ve and Gram-ve bacteria. On the other hand, non-polar extracts (petroleum ether extract and chloroform extract) did not show any antibacterial...
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Posted by admin on Dec 1, 2011 in |
The antidiabetic effects of Ethyl acetate (Et Ac), Petroleum-ether (Pet ether), and Chloroform fractions from methanolic extract of the seeds of Entada phaseoloides were investigated in alloxan induced diabetic mice (AIDM). The effect of these fractions (200 mg/kg body weight i.p) was observed on fasting blood glucose (FBG) level and active fraction was further investigated for its dose dependent activity (250 and 350 mg/kg b. w.) on fasting blood glucose level and also on total cholesterol (TC), triglyceride (TG), serum glutamate oxaloacetate transaminases (SGOT) and serum glutamate pyruvate transaminases (SGPT) level in AIDM and found significant effects. The most significant reduction of FBG level of around 72.02% was observed for Et-Ac fraction in AIDM. A significant reduction (*p< 0.05) in serum TC and TG level of 53.00% and 57.25% respectively was also found for Et-Ac fraction of E. phaseoloides. The hypoglycemic and hypolipidemic activities were comparable to metformin HCl (150 mg/kg). In diabetic mice, SGOT and SGPT levels were significantly elevated that were further reduced after intraperitoneal administration of...
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Posted by admin on Dec 1, 2011 in |
Ketoconazole (KTZ) is a synthetic antifungal drug used to prevent and treat fungal infections, especially in immunocompromised patients such as those with AIDS. Resistance to ketoconazole has been observed in a number of clinical fungal isolates, including C. albicans. Thus new effective agents with less toxicity against fungal infection are urgently required. With this view, ketoconazole thiosemicarbazone analogues (Compounds 1-10) were synthesized wherein condensation of different thiosemicarbazides substituted by different cyclic and aromatic amines with the KTZ was done. Investigation of in-vitro antifungal activity of compounds was done by broth microdilution assay method against five pathogenic fungi Aspergillus niger, Candida albicans, Candida krusei, Candida glabrata and Candida tropicalis. Ketoconazole was used as reference for inhibitory activity against fungi. All the compounds were found potent antifungal agents, while compounds 8, 9 and 10 exhibited excellent in-vitro antifungal activity showing importance of halogenated...
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Posted by admin on Dec 1, 2011 in |
Amaranthus vegetable and seeds are highly nutritious. Four species of Amaranthus were planted in pots in a glass house and leaves were harvested. Fresh leaves were analyzed for protein and carbohydrate content and oven dried leaves for Fe, Ca, K and Na contents. Protein content was varied from 6.10-9.00g/100g of fresh leaves. The amount of carbohydrate in fresh leaves of all four species varied from 9.75g-21.29g. Among the species, A. spinosus showed higher amount (21.29g) which is almost two folds higher than A. tricolor (9.75g). Results showed that A. viridis had higher accumulation (54mg). Overall K amount in the dry leaves of the four species varied between 2230-3900mg per 100 g of dry leaves and it was almost 2 fold differences between values of lowest and highest accumulation. The variation in amount of Ca was 38 fold higher, A. spinosus from the minimum amount (A. blitum). Fe content in dry leaves was maximum in A. viridis (15mg per 100g of dry wt) followed by A. spinosus (13.28mg), A. tricolor...
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Posted by admin on Dec 1, 2011 in |
A rapid and sensitive high performance liquid chromatography method for determination of cetirizine dihydrochloride and ambroxol hydrochloride has been developed. The chromatography system used a reversed phase C-8 column with UV- Vis detection at 230 nm. Mobile phase consisted of acetonitrile – 0.1% triethlamine (50:50 v/v) (pH adjusted to 4.0 using 10% ortho phosphoric acid) at a flow rate of 1.5 ml/min using propranolol as internal standard (I.S.). The calibration curve was linear in the concentration range of 2-20 µg/ml for cetirize dihydrochloride, 24-240µg/ml for ambroxol hydrochloride. The lower limit of detection was found to be 0.06 µg and 0.02µg, for cetirizine dihydochloride and ambroxol hydrochloride...
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