Posted by admin on Nov 1, 2011 in |
Analysis of fatty acid composition in fixed oil extracted from Sterculia foetida Linn. (Sterculiaceae) seed was done with the help of GC-MS. The structural identification was done by preparing FAME of the fixed oil and then subjected to GC-MS wherein Flame Ionization Detector was used. The oil constituted of 7 fatty acids whose carbon chain length, degree and position of unsaturation were determined from the characteristic ionization and fragmentation of FAME resulting from GC-MS electron impact, chemical ionization modes. The fatty acids found in the oil were methyl esters of tetradecanoic acid (Myristic acid) (1.65%), hexadecanoic acid (Palmitic acid) (11.87%), 9-Octadecenoic acid (Oleic acid) (20.50%), 7, 10-Octadecadienoic acid (Linoleic acid) (20.50%), 8-(2-Octacyclopropen-1-yl)octanoic acid (Sterculic acid)...
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Posted by admin on Nov 1, 2011 in |
The objective of this work was to study the effect of concentration and viscosity grade of HPMC, different diluents and inclusion of solid dispersions on the matrix tablets of aceclofenac. In present study, aceclofenac, a novel NSAID used for symptomatic treatment of pain and inflammation was formulated into matrix tablets with HPMC of two different viscosity grades (E50 LV and K15 M) by direct compression method. Before compression the formulations were evaluated for angle of repose, % compressibility and Hausner’s ratio. Tablets were evaluated for hardness, friability, weight variation, uniformity of thickness and diameter, and drug content. All pre-compressional and post-compressional parameters were found within the official limits. In vitro drug release studies were conducted for a period of 8 hrs using USP paddle method (II) at 37±0.5oC and at 75rpm speed in pH 7.2 phosphate buffer. Type of polymer and its concentration has influenced the drug release from matrix tablets. With increasing concentrations and increasing polymer viscosity the release rate decreased. Release rate increased with the addition of...
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Posted by admin on Nov 1, 2011 in |
Umbelliferone is a widely occurring phenolic compound of plant origin, for which many biological activities against chronic diseases have been reported. A comparative study of HPLC and HPTLC method has been developed for the quantitation of umbelliferone from dry tuber powder of Ipomoea mauritiana Jacq. Both the methods were validated for linearity, precision, limit of detection (LOD), limit of quantitation (LOQ), system suitability and accuracy in accordance with ICH guidelines. The accuracy of the HPLC and HPTLC method was checked by a recovery study conducted at three different levels and values of the average percentage recoveries for these two methods were found to be 97.90 and 98.90 respectively for umbelliferone. The study showed that both the methods are of similar efficiency, sensitivity and can be used for determination umbelliferone from dried tuber powder of Ipomoea mauritiana...
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Posted by admin on Nov 1, 2011 in |
The use of medicinal plants in the world and especially in India, contributes significantly to primary health care. Whole plant of the Cynodon dactylon is traditionally used to treat painful and inflammatory condition. We have undertaken this study since no detailed scientific study was available regarding the antifungal and antibacterial activity of the aqueous extract of the whole plant. In this study the aqueous extract of Cynodon dactylon was used to determine the antimicrobial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Proteus mirabilis and Candida albicans. The antimicrobial activity was determined using the agar well diffusion method. The diameter of the clear zone of inhibition surrounding the well was measured. The aqueous extract of Cynodon dactylon had antimicrobial activity against all the test organisms except Candida albicans. Phytotoconstituents present included Saponins, Tannins, steroids and Flavonoids. It can be concluded that aqueous extract of whole plant of Cynodon dactylon may be considered as an antibacterial agent and can be used to source antibiotic substances for possible treatment...
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Posted by admin on Nov 1, 2011 in |
The potential of methanol extract as a potent antimicrobial agent has been observed to be an effective means to combat disease causing pathogenic organisms. Growth pattern of Escherichia coli cells showed that the growth of cells decreased with an increase in concentration of extract. The specific relationship between the specific growth rate and the substrate concentration was thus be obtained by fitting these data into Monod kinetic model. The non competitive and uncompetitive inhibition kinetic models were also tested in order to determine the inhibitory effects of the methanol extract of roots on Escherichia coli. Longest lag phase of 8 hrs was obtained at nutrient broth concentration of 2 g/L. Kinetic studies showed that with an increase in concentration of extract (10-30 g/L) the specific growth rate and inhibition constant decreased but saturation constant increased. Therefore the biokinetic constant estimated by these models have laid the possibility of obtaining suitable antibiotic from Alstonia...
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