Posted by admin on Aug 1, 2011 in |
Nitrogen plays a pivotal role in growth and differentiation of cells of Phyllanthus amarus Schum. and Thonn. Both form and amount of nitrogen in nutrient medium have significant effects on the rate of cell growth and differentiation. In this study, effects of different inorganic nutrients (KNO3 and NH4NO3) and organic nutrients (glycine) on regeneration of P. amarus were evaluated. Murashige and Skoog’s medium (MS) was used as basal medium along with plant growth hormones and different concentrations of nitrogen. Among the various growth hormones tried, BAP (0.5 mg/l) gave maximum number of shoots (17.33±0.86) after 3-4 weeks of inoculation. However, various strength of KNO3, NH4NO3 and glycine were optimized in the medium in order to get the maximum number of shoots, nodes per shoot, shoot length as well as the size of leaves respectively. Amongst all modifications, less or more, MS medium with standard amount of nitrogen source was found to be most effective. The shoots regenerated on MS medium along with BAP (0.5 mg/l) were subjected to their elongation on the same medium...
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Posted by admin on Aug 1, 2011 in |
n-octacosanol is a normolipidemic aliphatic primary alcohol obtained from the plant Tinospora cordifolia Miers. In the present study, the plant stems were collected from five accessions namely Anniyalam, Chennai, Hassan, Vishakhapatnam and Bangalore between June and August. Later a simple HPTLC method has been established for determination of n-octacosanol in methanolic extracts of all these geographical sources using Toluene: Ethyl acetate: Formic acid (4.5:4.5:1 v/v) as mobile phase. Detection and quantification were performed by densitometric scanning at λ = 555 nm. The results of HPTLC estimation showed variation in n-octacosanol content in all the accessions. The extract from Anniyalam accession showed significantly higher amount of n-octacosanol (6.54%) and that of Vishakhapatnam showed the least (2.28%). Thus the present work is helpful in selecting Tinospora cordifolia yielding highest percentage of...
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Posted by admin on Aug 1, 2011 in |
Substituted Quinoxaline have received considerable attention during last two decades as they are endowed with variety of biological activities and have wide range of therapeutic properties. A literature survey indicates that quinoxaline derivatives possess different pharmacological and biological activities, of which the most potent is anti-inflammatory activity. In view of above literature survey, we thought to synthesize a novel substituted quinoxaline...
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Posted by admin on Aug 1, 2011 in |
The present study was undertaken to formulate directly compressible tablets to investigate the effect of different disintegrating agents such as maize starch and avicel PH101 on disintegration time, in vitro drug dissolution and their release properties. Tablets of paracetamol and the combination of paracetamol and diclofenac using maize starch and avicel PH 101 were prepared separately by direct compression method. The in vitro release of these formulations were studied at distilled water for one hour using the USP paddle method as specified in united states pharmacopeias for immediate release preparation. Significant differences were found among the drug release profile from different tablet formulations using different disintegrants. Formula No. 3 containing the combination of paracetamol and diclofenac using maize starch showed the lowest hardness and disintegrating time i.e. 3.99 Kg/cm2 and 7.08 minutes respectively, and the highest drug release i.e. 75.50% compared to other formulations. The influence of different disintegrants was evaluated by measuring the disintegration time and hardness of the tablets. The effect of starch & avicel on the...
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Posted by admin on Aug 1, 2011 in |
This study evaluated the in vitro antioxidant and cytotoxic potential of methanolic extract of the root of Calotropis procera (R. BR.). The Antioxidant activity of the extract was investigated by the In-vitro Anti-Oxidant activity (DDPH Scavenging activity) assays. The IC50 of the Calotropis procera studied by the above mention method was found below 100 μg/ml which indicates the potent antioxidant activity of the plant. The extract of the root of Calotropis procera also demonstrated a strong cytotoxic activity against brine shrimp nauplii with an LC50 value of 2.931...
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