Posted by admin on Jul 1, 2011 in |
Purpose: The present study was undertaken to investigate diuretic effect of aqueous and methanol extracts of the dried seeds of erythrina indica in normal rats. Method: Aqueous and methanol extracts of erythrina indica seeds were administered to experimental rats orally at doses of 25 and 30 ml/kg p.o. Furosemide (10 mg/kg) was used as positive control in the study. The diuretic effect of the extracts was evaluated by measuring urine volume, sodium and potassium content, conductivity and chloride content. Result: Urine volume was significantly increased by the two doses of aqueous and methanol extracts from root and bark of erythrina indica in comparison to control group. While the excretion of sodium was also increased by both extracts, potassium excretion was only increased by the aqueous extract at a dose of. 25 ml/kg. There was no significant change in the conductivity and pH of urine after administration of the erythrina indica extracts. The diuretic effect of the extracts was comparable to that of the reference standard (Furosemide). Conclusion: We can...
Read More
Posted by admin on Jul 1, 2011 in |
A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Teicoplanin in Parenteral dosage form. An Xterra RP C18, 250×4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.02M Potassium Dihydrogen Orthophosphate + 0.02M Dipotassium Hydrogen Orthophosphate + 0.5% Triethyl Amine in water pH: 3.2 adjusted with Orthophosphoric Acid: Acetonitrile (40:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.5 ml/min and the effluents were monitored at 210 nm. The retention time was 4.178 min. The detector response was linear in the concentration of 24.1-289.2 mcg/mL for Teicoplanin, the respective linear regression equation being Y (-242937.9235) = 71026.949x + 174544.8363 for Teicoplanin. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1.205 mcg/mL and 3.615 mcg/mL respectively for Teicoplanin. The percentage assay of Teicoplanin was 99.64 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple,...
Read More
Posted by admin on Jul 1, 2011 in |
Phytochemical constituents present in the aqueous and ethanolic extract of Syzygium cumini seeds were investigated which were found to be responsible for its medicinal efficacy. The major phytoconstituents present in S. cumini seeds were further determined by GCMS analysis which showed the presence of polyphenols, sesquiterpenes, n-alkanes. The antibacterial activity of ethanolic extract of S. cumini seeds were tested against common human pathogens by agar well diffusion method and minimum inhibitory concentration required to inhibit the growth of various pathogens were also evaluated. The seed extract was found to have high antibacterial activity. The total polyphenolic content of the ethanolic extract of S. cumini seed was determined by Folin-Ciocalteau method. The antioxidant activity of S. cumini seed ethanolic extract was evaluated by DPPH free radical scavenging assay, reducing power assay and total antioxidant capacity. S. cumini seed extract was found to have very high and potent antioxidant activity and might be due to the presence of high phenolic components in seed. The cytotoxicity assay of S. cumini seed ethanolic...
Read More
Posted by admin on Jul 1, 2011 in |
Mycobacterium tuberculosis is a deadly infectious disease, there is rising death of humans every year because of this disease, availability of genome sequences of Mycobacterium tuberculosis has provided tremendous amount of information that can be useful in drug target and new vaccine development. Sequence similarity provides accurate annotation for genes in newly sequenced genomes. In this present work about 107 hypothetical proteins of Mycobacetrium tuberculosis were taken and its functions were predicted using bioinformatics tools BLAST, BLOCKS, COGs, InterProScan and PFP. From our analysis of 107 hypothetical proteins only two shows 100% functions these proteins may serve as target for few...
Read More
Posted by admin on Jul 1, 2011 in |
In the present experiment effect of electroplating industrial effluent chromium on the behavioral responses of freshwater air-breathing cat fish Mystus cavasius was studied. The toxicity of chromium to aquatic life is strongly influenced by the form of chromium and quality of water. The two most prevalent chromium forms found in plating industry waste water are hexavalent chromium (Cr+6) and trivalent chromium (Cr+3). The fishes exhibited various behavioral responses – locomotory, physiological, neurological and morbidity responses under influence of chromium industrial effluent. One of the major advantages of using data on behavioral effects is that they are more sensitive indicators of potential for impacts on survival in the filed than are measures of lethality. Study noted the possible utility of the behavior responses as rapid biomonitory assessment technique for qualitative evaluation of various industrial...
Read More
