Posted by admin on Mar 1, 2011 in |
The aqueous, methanolic and saponin extracts of Zizyphus mauritiana barks has been screened for antimicrobial activities against some human vaginal pathogens Staphylococcus aureus, Pseudomonas aeruginosa, streptococcus facecalis, klebsiella pneumoniae, Escherichia coli, Enterobacter faecalis, Enterobacter faecium and Proteus mirabilis isolated from patient samples. Extracts were found to produce significant inhibition against all the pathogens. Saponin extract were observed to be more active than methanolic and aqueous fraction. Extracts are found to be more active against Staphylococcus aureus and Escherichia coli...
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Posted by admin on Mar 1, 2011 in |
Tinospora cordifolia, belonging to the Menispermaceae family, has been investigated for isolation of its secondary metabolites and evaluation of biological activities with special emphasis to the antimicrobial screening and cytotoxic study. The crude methanolic extract of Tinospora cordifolia along with its Pet ether, carbon tetrachloride, and chloroform soluble fractions were subjected to microbiological investigation by the disk diffusion method. The CCl4 soluble fraction was found to have mild to moderate anti-microbial action. In the brine shrimp lethality bioassay, the Pet ether, CCl4, chloroform and aqueous soluble fractions of crude methanolic extract were found to show LC50 1.918 mg/ml, 0.402mg/ml, 0.691 mg/ml and 2.246 mg/ml respectively. This indicated that the carbon tetrachloride and chloroform fractions were highly cytotoxic....
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Posted by admin on Mar 1, 2011 in |
Diabetes mellitus is characterized by persistent fasting and postprandial blood glucose levels due to inability of the body cells to utilize glucose properly. Though drugs are plenty for the treatment of diabetes, none is found to be ideal due to undesirable side effects and diminution after prolonged use. Hence, search for novel drugs, especially from plant origin continues. Based on folkloric use, the present study was designed to evaluate the antidiabetic and antioxidant potential of Annona squamosa Linn. (Annonaceae) in STZ-induced experimental diabetes in rats. Daily oral administration of Annona squamosa leaves extract (100 mg/kg b.w./day) to diabetic rats for 30 days significantly reduced the levels of blood glucose, glycosylated hemoglobin, urea and creatinine. The observed decrease in the levels of plasma protein, plasma insulin, C-peptide and hemoglobin in the diabetic rats were elevated to near normal by the extract treatment. The altered antioxidant status of diabetic rats were reverted back to near normalcy by the administration of Annona squamosa leaves extract. The efficacy of the Annona squamosa extract...
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Posted by admin on Mar 1, 2011 in |
A simple reverse phase liquid Chromatographic method has been developed and subsequently validated for simultaneous determination of Pioglitazone and Glimepiride in combination. The separation was carried out using a mobile phase of phosphate buffer (pH-4.5): Acetonitrile (45:55) v/v and using methanol as diluent. The column used was Inertsil ODS (250 mm x 4.6 mm i.d., 5μm) with flow rate of 1 ml/min using UV detection at 225 nm. The described method was linear over a concentration range of 5-50µg/ml and 5-25 µg/ml for the assay of Pioglitazone and Glimepiride respectively. The retention times of Pioglitazone and Glimepiride were found to be4.6 and 7.7min respectively. Results of analysis were validated statistically and by recovery studies. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Pioglitazone and Glimepiride bulk drug and in its pharmaceutical dosage form....
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Posted by admin on Mar 1, 2011 in |
To deliver zidovudine (AZT) to asialoglycoprotein receptors, drug-containing galactosylated liposomes were prepared. Simultaneously, miconazole nitrate (MCZ) was entrapped in the outer liposomal bilayer of double liposomes for the treatment of opportunistic fungal infections. Galactosylated liposomes were entrapped in double liposomes. The galactose binding Ricinus communis lectin was used for the determination of in vitro ligand binding capacity. Percent cumulative drug release from double liposomes with inner uncoated liposomes (DLMA) and inner galactosylated liposomes (Gal-DLMA) were compared. Cellular drug uptake studies were performed. In vivo antifungal activity, plasma distribution and hepatic localization study were performed in albino rats. Percent cumulative drug release of AZT from inner liposomes was significantly higher with DLMA as compared to Gal-DLMA. MCZ uptake was 4.7 times greater after encapsulation in liposomes irrespective of inner liposome coating and maximum AZT uptake (7.9 fold enhancement) was observed from double bilayer galactosylated liposomes as compared to free drug. MCZ encapsulated in double liposomes was able to reduce CFU (colony forming units) values to a significant extent in various...
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