Posted by admin on Feb 1, 2011 in |
Purpose: A study was conducted for Arthritis, mainly focused on Osteoarthritis (OA) and Rheumatoid Arthritis (RA), to check the prevalence, awareness, severity, probable causes, drug utilization and evaluate efficiency of different therapies in population of North Gujarat. Methodology: Detailed questionnaires were designed to collect data randomly from 260 patients of rural and urban area of Palanpur and Mehsana districts. Results: Prevalence of OA was found to be 3.11% where as that of RA was 0.06%. Furthermore, the prevalence ratio (male: female) for OA was 1:1.19, where that of for RA was 1:4. Prevalence of OA and RA is significantly higher in urban area than in rural area. Suffering for most arthritic patients is more than five years indicating its chronic nature. The usage of Allopathic medicine for the treatment for OA and RA is highest as compared to other therapies. Family history for OA and RA patients was found 20% and 28.57% respectively indicating hereditary factor for diseases. More numbers of parts affected in RA patients than that of...
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Posted by admin on Feb 1, 2011 in |
This study is concerned to develop a rapid system for regenerating shoots and callus from mature shoot tip explants of Asteracantha longifolia (L.) Nees, a medicinally important annual herb (family acanthaceae). Effective shoot and callus regeneration was observed by using several concentrations of cytokinins and auxins with MS medium. Shoot tips responded better for callusing almost in all combinations such as BA + IAA (1.0+1.0, 1.5+1.0, 1.5+1.5, 2.0+1.5 mg/lit) and BA + NAA (1.0+1.0, 1.5+1.0, 1.5+1.5, 2.0+1.5 mg/lit). Shooting was observed only in shoot tip explants when media was supplemented with growth regulators in different combinations at different concentrations such as BA (0.5, 1.0, 1.5, 2.0 mg/lit) and BA+NAA (1.0+0.5, 1.5+0.5, 1.0+1.0, 1.5+1.0). However no shooting was observed in MS media by using nodal explants containing different concentrations of BA +NAA + IAA. This approach of plant tissue culture may proved to be a helpful method to develop a rapid system of regeneration for production of medicinally important products. Also the production of aseptic plants which may decline the...
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Posted by admin on Feb 1, 2011 in |
A simple, rapid and reproducible HPLC method was developed and validated for the estimation of Formoterol Fumarate Rotacaps. Symmetry C8 (4.6×150mm, 5 µ) column, in isocratic mode with mobile phase containing Ammonium Acetate Buffer pH 5.0 and Acetonitrile (80:20) was used. The flow rate was 1.0 ml/min and the analyte was monitored at 254 nm. The retention time for Formoterol was about 4.6 mins. The system was validated for precision, accuracy, linearity, limit of detection and limit of quantitation. The Linearity was obtained in the concentration range of 30µg/ml to 180µg/ml with correlation coefficient of 0.999. The percentage recovery of Formoterol was found to be in the range of 98% -102%. Therefore it was concluded that the proposed method can be used for routine analysis of Formotero Fumarate Rota...
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Posted by admin on Feb 1, 2011 in |
Ayurvedic medicine plays a crucial role in healthcare and serves the health need of a vast majority of people in developing countries. Some clinically used ayurvedic herbal preparations were investigated (solvent extract preparation) for antibacterial potential against enteric bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus epidermidis, Salmonella typhimurium and Proteus vulgaris. In present study Shatawaryadi churna, Panchasakar churna, Talisadi churna, Dashmula churna and Manjistadi churna were potent antibacterial against Staphylococcus epidermidis, Proteus vulgaris, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Study suggested thatthese herbal preparations may be useful as an alternative medicine in the treatment of enteric bacterial...
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Posted by admin on Feb 1, 2011 in |
Crude ethanolic extract of Brassica nigra was studied to detect the chemical compounds as well as to evaluate the antioxidant and anti-inflammatory activities. The different antioxidants assays including total antioxidant activity, DPPH, nitric oxide (NO) scavenging, reducing power, total phenolic content and flavonoid content were studied. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides and carbohydrates in the extract. Total phenol content in the plant was 6.67 mg/g of galic acid. Brassica nigra was found to contain 2.04 mg/g of quercetin in flavonoid assay. Total antioxidant capacity of the extractwas found to be 97.08 mg/g of ascorbic acid. Brassica nigra showed IC 50 value of 63.09µg/ml whereas the standard antioxidant showed IC 50 value 14.45 µg/ml in DPPH method. The standard antioxidants ascorbic acid, galic acid and quercetin showed the reducing power 485.75%, 736.30% and 763.01%, respectively whereas Brassica nigra showed the value 263.69%. IC50 value in NO scavenging activity of the extract was found to be 118.21 µg/ml whereas ascorbic acid showed the value 5.47 µg/ml and...
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