Posted by admin on Jan 1, 2011 in |
Antipyretic effect of petroleum ether and chloroform soluble fractions of ethanol extract of the roots of Vernonia cinerea was investigated. Intraperitoneal administration of boiled milk at a dose 0.5 ml/kg body weight in albino rabbit leads to pyrexia. Intraperitoneal (i.p. route) administration of petroleum ether and chloroform soluble fractions of ethanol extract of the roots of Vernonia cinerea at a dose 250 mg/kg body weight were shown significantly reduce the elevated body temperature of rabbit which was compared with standard aspirin (market product) and solvent...
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Posted by admin on Jan 1, 2011 in |
Tylophora indica is a perennial climbing plant native to India, found in plains, forests, hills of southern and eastern India. The portions of plant used medicinally are leaves. The Total phenolic content of methanolic leaves extract was estimated by Folin-Ciocalteu assay method, and was found to be 0.160 mg/CE/g (Catechine Equivalent per gram). However, antioxidant activity of methanolic leaves extract of Tylophora indica was determined by DPPH free radical scavenging method. The DPPH radical scavenging activity of methanolic extract of Tylophora indica was found to be highest at 100μl concentration which was 30.74%. Nevertheless, % DPPH scavenging activity of standard ascorbic acid at same concentration was found to be 45.43%. The % DPPH scavenging activity increases with the increasing concentration. The concentration of Tylophora indica needed for 50% inhibition (IC50) was found to be 199.58μg/ml whereas 194.58 μg/ml needed for ascorbic...
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Posted by admin on Jan 1, 2011 in |
A rapid and simple high performance thin layer chromatography (HPTLC) method with densitometry at 230 nm was developed and validated for simultaneous determination of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation. Separation was performed on aluminum-backed silica gel 60F254 HPTLC plates as stationary phase and using a mobile phase comprising of methanol:distilled water:formic acid in the volume ratio of 0.5:7.5:0.1 (v/v/v), respectively. After development, plates were observed under UV light. The detector response was linear in the range of 200-1200 ng/spot and 100-1000 ng/spot for Naproxen sodium and Sumatriptan succinate. The validated lowest limit of detection was 85 ng/spot and 40 ng/spot whereas lowest limit of quantification was 200 ng/spot and 100 ng/spot for Naproxen sodium and Sumatriptan succinate, respectively. The percentage assay of Naproxen sodium and Sumatriptan succinate was found between 99.25 and 98.03 % respectively. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation and...
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Posted by admin on Jan 1, 2011 in |
Meloxicam (MEL) is an oxicam derivative and a member of the enolic acid group of non-steroidal anti-inflammatory drugs (NSAIDs). Most of the reported methods for HPLC analysis of Meloxicam are cumbersome, time-consuming and expensive. Reverse phase chromatographic analysis was performed on a C18 Hi Q Sil column with methanol–water- orthophosphoric acid (80:19.9:0.1 % v/v) at a flow rate of 1ml/min and detection wavelength of 360 nm. System suitability tests essential for the assurance of quality performance of the method were performed. The method was validated for accuracy, precision, reproducibility, specificity and robustness, limit of detection (LOD) and limit of quantification (LOQ). A single sharp peak was obtained for MEL at Rt of 4.38 ± 0.02 min. The polynomial regression data for the calibration plots exhibited linear relationship (r = 0.999) over a concentration range of 4–20µg/ml and the linear regression equation was y = 43754x – 2094. Accuracy ranged from 99.33 to 100.45% and the % coefficient of variation (CV) for both intra-day and inter-day precision was less than...
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Posted by admin on Jan 1, 2011 in |
Lornoxicam (chlortenoxicam) is a non steroidal anti- inflammatory drug (NSAID) of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. It is available in oral and parental dosage formulation. Lornoxicam is a Yellow or slightly yellow powder. It is slightly soluble in water, soluble in hydrochloric acid, slightly soluble in methanol. A simple spectrophotometric method was developed for the determination of lornoxicam in pharmaceutical tablet dosage form. Lornoxicam exhibiting λ max at 380 nm in mobile phase (phosphate buffer: methanol) in ratio of 50:50 and obeyed linearity in the concentration range of 1-20mcg. The proposed method was statistically...
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