New 5- phenyl- 1, 3, 4- oxadiazole- 2- thiol derivative was synthesized by the ring closure reactions of benzohydrazides with carbon disulphide in presence of ethanolic KOH followed by substitution with secondary amines at 2nd position. All the newly synthesized compounds were characterized by IR, NMR and LC-MASS spectral data. Most of them were tested for their anti-inflammatory and antibacterial...
Read MoreHormonal changes during the menstrual cycle are well documented but there are very few reports on the changes in serum calcium, phosphorus and uric acid levels in various phases of the menstrual cycle. Therefore, present study was conducted on 50 healthy females in the age group of 18-35 menstruating regularly (cycle length between 25 and 35 days). The study demonstrated that serum calcium levels were significantly (p<0.001) higher in follicular phase than menstrual and luteal phases. Serum inorganic phosphorus levels decreased progressively and significantly (p<0.001) from the menstrual phase to the follicular phase and from the follicular phase to the luteal phase while serum uric acid levels were significantly (p<0.001) lower in luteal phase than the menstrual and follicular phases. These changes in calcium, phosphorus and uric acid in serum may have significance in terms of the normal reference interval, hence necessitate small but significant alterations to the normal reference interval for these analytes in menstruating...
Read MoreA simple, precise, specific and accurate reverse phase HPLC method has been developed for the determination of Ritonavir in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved on Symmetry C18 (4.6 x 100mm, 3.5 μm) column using a mixture of Buffer: Acetonitrile (50:50) as the mobile phase at a flow rate 1.0 ml/min. Linearity was observed in concentration range of 50-150μg/ml. The retention time of Ritonavir was 5.1 min. The analyte was monitored using UV detector at 239 nm. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and...
Read MoreA considerable number of studies have already been performed involving the roots and stems of Berberis aristata and thus the following investigation has been carried out for the phytochemical study of ethanolic extract of heartwood of Berberis aristata. The heartwood (1.8 kg) of Berberis aristata DC. Var aristata (Berberidaceae) was exhaustively extracted in 95% ethanol using Soxhlet apparatus. The column was prepared for isolating various phytoconstituents using solvents of increasing polarity. On the basis of various spectral data analysis i.e., IR, 1HNMR, 13CNMR and +ve ion FAB MS, one of the isolated phytoconstituents was characterized as an aliphatic hydrocarbon,...
Read MorePurpose: A study was conducted for Arthritis, mainly focused on Osteoarthritis (OA) and Rheumatoid Arthritis (RA), to check the prevalence, awareness, severity, probable causes, drug utilization and evaluate efficiency of different therapies in population of North Gujarat. Methodology: Detailed questionnaires were designed to collect data randomly from 260 patients of rural and urban area of Palanpur and Mehsana districts. Results: Prevalence of OA was found to be 3.11% where as that of RA was 0.06%. Furthermore, the prevalence ratio (male: female) for OA was 1:1.19, where that of for RA was 1:4. Prevalence of OA and RA is significantly higher in urban area than in rural area. Suffering for most arthritic patients is more than five years indicating its chronic nature. The usage of Allopathic medicine for the treatment for OA and RA is highest as compared to other therapies. Family history for OA and RA patients was found 20% and 28.57% respectively indicating hereditary factor for diseases. More numbers of parts affected in RA patients than that of...
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