Posted by admin on Apr 1, 2011 in |
Nanodrop spectrophotometric method was developed and validated for the estimation of Ranolazine in bulk. Ranolazine exhibited λmax at 272nm in water and obeyed linearity in the concentration range of 12.5-2000 ppm. The proposed method has been applied successfully for the analysis of Ranolazine in bulk with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of Ranolazine in...
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Posted by admin on Apr 1, 2011 in |
The in vitro activity of the petroleum ether extract of fruit part of Phyllanthus acidus was tested for cytotoxic antibacterial and antioxidant activities as well as for phytochemical screening. The plant was collected from Savar, Dhaka. Phytochemical screening of petroleum extract of phyllanthus acidus revealed the presence of carbohydrate, glycoside and steroid. The extract exhibited antibacterial activity was determined by the disc diffusion method against thirteen pathogenic bacteria and the cytotoxic activity was performed by brine shrimp lethality bio-assay method. The higher concentrations showed antimicrobial activity against a number of bacteria including Salmonella typhi, Staphylococcus aureus, Bacillus cereus, Escherichia coli, Bacillus megaterium. In brine shrimp lethality bio-assay, the LC50 value was 3.12. The phenolics contents was 159.601 mg/g GAE and the amount of flavonoid was 24.183 mg/g of quercetin equivalent. The DPPH radical scavenging activity of Phyllanthus acidus was found to slight increase with increasing concentration of the extract and IC50 value showed 1192.263 µg mL−1 for plant extract compared to 13.37 µg mL−1 which was the IC50 value...
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Posted by admin on Apr 1, 2011 in |
This study was aimed to investigate analgesic and neuropharmacological actions of the methanolic extract of rhizomes of Hedychium coronarium. The analgesic activity was evaluated for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test in mice respectively. The extract, at the doses of 100, 200 and 400 mg/kg body weight, produced a significant increase in pain threshold in tail immersion methods in a dose dependent manner. In acetic acid-induced writhing test, the extract at 400 mg/kg dose showed a maximum of 73.12% writhing inhibition (p <0.001) compared to the control and this activity was comparable to 75.78% inhibition of writhing by standard drug Diclofenac-Na (25 mg/kg). The extract was also investigated for its neuropharmacological action using hole-cross and open field test in mice. The extract displayed dose dependent suppression of motor activity and exploratory behavior in mice in the tested models. The results of the study indicate that the plant possesses strong analgesic and central nervous system depressant...
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Posted by admin on Apr 1, 2011 in |
The antimicrobial effects of selected medicinal plants commonly used by herbal practitioners in central province Kenya was evaluated on different bacterial strains- Staphylococcus aureus (Gram +ve cocci) – ATCC 20591, Salmonella typhi (Gram –ve rod) – ATCC 2202, Escherichia coli (Gram-ve rod) – STD. 25922, Klebsiella pneumoniae (clinical isolate) and Pseudomonas aeroginosa (Gram-ve rod) – ATCC 25852. Also Candida albicans ATCC EK138 was used as a fungal isolate. Methanol was used as the only solvent in the extraction. The in vitro antimicrobial activity was performed by agar disc diffusion method. The most susceptible Gram-positive bacteria was S. aureus (between 19.33-23.33mm), while the most susceptible Gram-negative bacteria was P. aeruginosa (14.66-19.33mm). All the extracts showed sufficient inhibitory activity to the test strains. The Gram positive strain (S. aureus) was more sensitive to the extracts (range 23.33-19.33mm) than the Gram negative strains (range 21.00-14.66mm). The mean inhibition value was between 15.997mm and 19.995mm. Statistical analysis revealed that Hyptis spicigera and Crotalaria quartiniana produced significantly different (P≤0.05) zones of inhibition in all...
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Posted by admin on Apr 1, 2011 in |
The present study was aimed at investigating the antioxidant activities of coumarin in streptozotocin (STZ)-nicotinamide (NA) induced type 2 diabetic rats. Diabetes was induced in experimental rats by a single intraperitoneal (i.p.) injection of STZ (45 mg/kg body weight (b.w.)), 15 min after the i.p. administration of NA (110 mg/kg b.w.). Diabetic rats were administered coumarin intragastrically at 100 mg/kg b.w. for 45 days. The antioxidant activities of coumarin have been evaluated by using a range of in vivo assays and in vitro assays. In case of in vivo studies the levels of plasma thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (HP) and conjugated dienes (CD) were measured. The levels of vitamin C, vitamin E and reduced glutathione (GSH) in the plasma and tissues were assayed. In case of in vitro assays 2, 2¢-diphenyl-1-picrylhydrazyl radical (DPPH•), 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS•+), hydroxyl radical, superoxide anion scavenging activity and reducing power were measured. In addition to that ascorbic acid and butylated hydroxyl toluene were used as the reference antioxidant...
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