Posted by admin on Oct 1, 2010 in |
The present study was designed to investigate the effect of diosmin on the levels of glycoprotein components in plasma and tissues of streptozotocin (STZ)-nicotinamide (NA) induced diabetic rats. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of STZ (45 mg/kg b. w). Diosmin (DS) (100 mg/kg b. w) was administered orally for 45days. The effects of diosmin on plasma glucose, plasma insulin, plasma and tissue glycoproteins were studied. The levels of plasma glucose and plasma glycoproteins were increased significantly whereas the level of plasma insulin was significantly decreased in diabetic rats. There was a significant decrease in the level of sialic acid and elevated levels of hexose, hexosamine and fucose in the liver and kidney of diabetic rats. Oral administration of diosmin to diabetic rats led to decreased levels of plasma glucose and plasma glycoproteins. The levels of plasma insulin and tissue sialic acid were increased, whereas the levels of tissue hexose, hexosamine and fucose were near normal. The present study indicates that diosmin possesses...
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Posted by admin on Oct 1, 2010 in |
Modern medicines are gaining less attention due to their limited availability and affordability in human intestinal helminthesis. Thus, most of the world’s population depends to a greater extent on traditional medical remedies. Carissa spinarum, Linn. (Apocynaceae) is a small spinous, evergreen shrub growing throughout India in dry regions. Further, C. spinarum roots are traditionally used for their purgative properties as well as to treat worm infested wounds in animals. There is no report on pharmacologically evaluated antihelmintic activity of root extract of C. spinarum till date. Therefore, in the present study we have investigated the antihelmintic activity of methanolic, aqueous and chloroform extracts of root of C. spinarum on Pheretima posthuma. The fresh and dried root of C. spinarum were collected in the month of November from the Bilaspur region, Chhattisgarh state, India, and the antihelmintic activity was evaluated in terms of time taken to cause paralysis and death of the adult Indian earthworm Pheretima posthuma. Piperazine citrate (PC; 10 mg/ml) was included as reference compound. The present investigation...
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Posted by admin on Oct 1, 2010 in |
Bacopa monniera is locally referred as Brahmi Shak, has been reputed to use in the Ayurvedic system of medicine for centuries. In our ongoing study to identify the scavenger of ONOO- from natural sources, the aerial parts of Bacopa monniera Linn. was evaluated to scavenge authentic peroxynitrites (ONOO-) and to inhibit total reactive oxygen species (ROS) generation, in kidney homogenates using 2′,7′-dichlorodihydrofluorescein diacetate (DCHF-DA). A methanol (MeOH) extract of the B. monniera showed strong ONOO- scavenging activity in the ONOO- system ( IC50 = 11.23±2.14 µg/ml), and marginal activity in the total ROS systems (IC50 = 46.52±0.91µg/ml). So the MeOH extract was fractionated with several organic solvents, such as dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and n-butanol (n-BuOH). The EtOAc soluble fraction, showed strong activity in all the model systems tested. The Brine shrimp lethality bioassay method was used to determine the toxicity of the extracts where Vincristin sulphate was used as a positive control. The dichloromethane (CH2Cl2) fraction showed highest activity (LC50 = 19.02 ± 1.16 µg/ml) and other...
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Posted by admin on Oct 1, 2010 in |
The present paper deals with the microbial studies of the complex Co(II) & Cu(II) with antibiotic drug Tetracycline, a formula Co (C22 H24 N2 O8) MoO4 4H2O and Cu (C22 H24 N2 O8) MoO4 3H2O has been suggested on the basis of elemental analysis and molar conductance for the newly synthesized complex. The microbial studies of synthesized complex were studied on pathogenic bacteria using gram +ve (Bacillus subtilis and Staphylococcus aureus) and gram -ve (Shigella flexneri, Salmonella typhosa, Escherichia coli) and some fungi (Aspergillus flavus, Fusarium oxysporum, Chrysosporium pannicale, Alternaria solani, Candida albicans )....
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Posted by admin on Oct 1, 2010 in |
A simple, precise and reproducible liquid chromatography–tandem mass spectrometry method has been developed and validated according to the United States – Food and Drug Administration (US-FDA) guidelines for the quantitation of Montelukat in human plasma using Neverapine as an internal standard. Montelukast is a fast acting and potent cysteinyl leukotriene receptor antagonist which is being used in the treatment of asthma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. The chromatographic run time was 3.2 mins on a Agilent Zorbax SB C8, 150×4.6 mm, 5.0µm Column. The mobile phase was a mixture of Methanol: 0.1 % Formic acid in water (90:10 v/v). Inter-batch and intra-batch coefficient of variation across four validation runs for the quality control samples was less than 6.02%. The accuracy determined at quality control levels was within 90.98 –...
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