Posted by admin on Jan 1, 2011 in |
Dissolution rate enhancement of alprazolam, an anti-anxiety drug, was done by preparing solid dispersions by solvent evaporation method .Polyethylene glycol 6000 and Polyvinyl pyrrolidone k-30 were selected as carriers. Drug: Polymer ratios were taken as 1:1,1:2and1:4 for both the polymers for the preparation of solid dispersions. Tablets were prepared from solid dispersions and also from physical mixtures by direct compression. Dissolution rates and drug releases of solid dispersions or their tablets were found more than those of corresponding physical mixtures or their...
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Posted by admin on Jan 1, 2011 in |
This study describes development and subsequent validation of a reversed phase high performance liquid chromatographic (RP-HPLC) method for the estimation of ursodeoxycholic acid used to solubilize cholesterol gallstones, in conventional tablet dosage formulation and in prepared dosage form. The chromatographic system was achieved in a BDS Hypersil C8 column (Thermo, 250mm x 4.6 mm, 5 µ) with an isocratic mobile phase comprising of methanol, water and phosphoric acid (77:23:0.6 v/v). Since the drug shows moderate absorption only in the short UV wavelength region (200-210 nm), quantification of the amount released from the pharmaceutical preparations by simple UV spectrophotometry is hampered by possible interferences from formulation excipients and dissolution medium components. A new method has been developed utilizing the above mobile phase and detection in refractive index (RI) detector. In this chromatographic condition ursodeoxycholic acid was eluted about 3.0 min with no interfering peaks of excipients used for the preparation of dosage forms. The method was linear over the range from 240-360 µg/mL in raw drug (r2 = 0.995). The...
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Posted by admin on Jan 1, 2011 in |
Objective: To establish does response relationship to the hypertensive patients and ensure safety profile of drugs through counseling of patients. Method: Patients who have suffered by secondary hypertension with blood pressure range of above 145-170/100-110 mmHg are selected and divided in two groups. First group of patients to administer for Nifedipine 5mg and furosemide 40mg administered for second group patients by orally .Every 30minits of after 4hrs blood pressure monitored and compliance about disease and drugs from patients are collected and recorded. Finally statistically analyzed safety and does response of the drugs. Discussion: 30% of patients were found to be 111-115/90-95 mmHg after treated with nifedipine and 27.5%of patients blood pressure reduced into 116-120/95-100mmHg for after administration of furosemide. Conclusion: In this study it was found that nifedipine is effective in the management of hypertension in terms of efficacy than furosemide. We should recommend this regimen for treatment of hypertensive patients in...
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Posted by admin on Jan 1, 2011 in |
Xylanase enzyme, one example of secondary metabolites generated by the endophytic microbes was an enzyme which degrades Xylan. The current research study was focused on isolation of endophytic fungi from Dipterocarpus grandiflorus Blanco that produced xylanase enzyme and evaluation of this enzyme activity. Isolation of endophytic fungi from Twigs of D. grandiflorus Blanco were performed via direct seeding inoculation technique utilizing Corn Meal Malt (CMM) medium, followed by purification of these isolates in Potato Dextrose Agar (PDA) media and macroscopic observations to characterize these isolates. The presence of xylanase enzyme was determined using Dinitro Salicylic Acid (DNS) method. Over 12 days our study results yield a total of eight (8) endophytic fungi isolates with six xylanase enzyme producing isolates (Dg II,3 a2, Dg II,3 a3, Dg II,3 b1, Dg II,3 c1, Dg II,3 e1, Dg II,3 f1). Highest extracellular xylanase activity was found in Dg II, 3fl (4.036 U/mL) while the lowest one was 0.426U/mL in isolate Dg II,...
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Posted by admin on Jan 1, 2011 in |
Extractive visible spectrophotometric method is described for the analysis of chloroquine in it pure forms, pharmaceutical dosage forms and biological materials. Linearity, precision, stability and accuracy were evaluated according to the recommended validation procedure. In the described method a drug complex was formed with cobalt thiocyanate reagent, buffered and extracted into nitrobenzene and absorbance determined at 625nm wavelength. Good linearity was obtained between 2µg/ml and 60µg/ml. The coefficient of variation of 5 determinations of a single sample of 2µg/ml was 1.2% and that of 0.02µg/ml was 20.3%. The mean coefficient of variation over the whole range of standard samples was 10.5+3.3% (s.d.). An assay of acceptable accuracy can be accepted down to a concentration of 0.02µg/ml. The intra- day and inter- day precision values were less than 2% for all samples analysed. The accuracy, as seen in or estimated from the percent recovery studies, was between 96 and 99% with an average of 97.75% in biological materials, common excipients and sampled tablets. Non- aqueous titration was employed as a...
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