Posted by admin on Jul 1, 2010 in |
The present work deals with development and standardization of phytochemical screening for quantification of methanolic extract of medicinal plant stem bark of Anthocephalus cadamba (Roxb.)Miq. The scientific parameter is necessary to identify the exact plant material and to find its quality and purity. The present study deals with various, physical evaluation and preliminary phytochemical screening of various successive extracts such as qualitative chemical analysis and different spectroscopic study were carried out and the parameters were reported. HPTLC of extract shows the seven different peaks confirming that the seven compounds present in methanolic extract of Anthocephalus cadamba. The alkaloidal content of stem bark of the title plant has been determined. These studies indicated the possible information for correct identification and standardization of this plant...
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Posted by admin on Jul 1, 2010 in |
A novel method was developed for the determination of fluoroquinolones such as ciprofloxacin hydrochloride (CPL) and ofloxacin (OFX) by isocratic reverse phase-high performance liquid chromatography (RP-HPLC) coupled with UV detection. The developed method was rapid, accurate, reproducible, economical and sensitive for the simultaneous estimation of ciprofloxacin hydrochloride and ofloxacin. Fluoroquinolone antibiotics were separated on an analytical column (SIL 100Å, 125×4.6 mm ID) C18-RP, 5 µm, at ambient temperature. The mobile phase was consisted of Phosphate Buffer (15 mM): Methanol: Acetonitrile: TEA (66:24:10:1% v/v), pH was 6.5 (adjusted with 80% HCl), at a flow rate 1ml/min and sample injection volume was 20-µL. UV detection at 289 nm and retention time (RT) was 3.01 and 6.03min for ciprofloxacin hydrochloride and ofloxacin respectively. The method was validated in terms of stabilizing accuracy, precision (Intra/Inter-day), linearity, specificity, stability and sensitivity. The proposed method has been successfully applied for the analysis of marketed tablets and can be used for the routine analysis of formulations containing any one of the above drugs without any alteration...
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Posted by admin on Jul 1, 2010 in |
The effect of Adenanthera pavonina (AP) seed extract on the blood cholesterol level of atherogenic diet rats was evaluated. Twelve adult male Wistar rats divided into 3 groups of 4 animals each were used and were treated orally with normal saline (Control group), atherogenic diet (Positive control) and 200mg/kg of AP seed extract (treatment) over a 4-week period. The present study was undertaken to assess body weight, total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and very low density lipoprotein (VLDL). They were significantly lower in the extract treated groups compared to the control group. Phytochemical screening showed that the extract contained cardiac glycosides, tannins, saponins, alkaloids and flavonoids. Cyanogentic glycosides and anthraquinones were absent. Histopathological examination showed that the extract did not cause any significant lesion changes in the liver, kidney and even the testes. The study showed that Adenanthera pavonina (AP) seed extract have the potential to cause a blood cholesterol lowering effect. The serum biochemistry changes may suggest that the extract...
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Posted by admin on Jul 1, 2010 in |
The genus Mangifera indica belongs to the family Anacardiaceae and comprises of 40 species distributed in tropical and sub-tropical parts of South East Asia, Africa and Latin America The resin is useful in cutaneous disease .the juice of kernel is snuffed to stop nasal bleeding. A decoction of the leaves with a little honey added is given in aphonia, the loss of voice. Mid ribs of the leaves calcined are used to remove warts on eyelids. Tender leaves, dried and made into a powder, are useful in diabetics. Smoke of the leaves is said to have a curative effect in some infections of throat diseases. Ashes of leaves are a popular remedy for burns and scalds. Dried flowers in decoction powder and useful in diarrhea, chronic dysentery and gleets .Powder of flowers are used for fumigation against mosquitoes. Gum resin of the tree is applied with benefit to cracked feet. Gum resin from the bark is used in catarrhs and when mixed with lime juice and applied, it is...
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Posted by admin on Jul 1, 2010 in |
Withania somnifera Dunal. (ashwagandha) is widely used in Ayurvedic medicine, the traditional medicinal system of India. It is an ingredient in many formulations prescribed for a variety of musculoskeletal conditions (e.g., arthritis, rheumatism), and as a general tonic to increase energy, improve overall health and longevity, and prevent disease in athletes, the elderly, and during pregnancy. In the recent past tremendous attempts are being made to evaluate the scientific standards for Traditional system of medicine. For the standardization of this drug Pharmacological and Phytochemical parameters were carried out such as moisture content, ash values, pH values, extractability in water and alcohol were carried out. Thin Layer Chromatography studies were also carried out to ascertain the quality of this...
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