Posted by admin on Nov 30, 2025 in |
The objective of this work was to create a polyherbal formulation and evaluate its antibacterial and wound-healing properties in an in-vitro setting. Physicochemical properties of a topical polyherbal formulation were assessed with the chitosan, gelatin, neem, pectin, honey, and aloe vera were combined in different proportions. Polyherbal formulations were assessed for their tensile strength, swelling behavior, moisture retention, microbial penetration, honey release, pH, folding endurance, thickness, water vapor transmission in the prepared formulation. In-vitro activity revealed that F5 formulation had the better wound healing potential as compared to F4 and F3. Thus, we can conclude that enhanced dressings with good wound healing capabilities can lead to new possibilities in the treatment of wound healing related to burns. The outcome of this investigation indicates that the film made with various herbs and 5.4 ml of honey meets all the specifications for wound dressings, including thickness, weight, and folding endurance. The polyherbal formulation has tensile strength of 26N/mm2, moisture uptake of 17.14 ± 1.12 to 26.7 ± 2.17%, and swelling behavior of 85.38. Its antibacterial...
Read More
Posted by admin on Nov 30, 2025 in |
Introduction: Antimicrobial resistance is a burning problem globally due to irrational use of antibiotics. Drug utilization study is a strong tool for evaluating rational use of antibiotics. Objective: To evaluate the drug utilization pattern, to assess the risk factors associated with Neonatal sepsis & to encourage rational prescribing for these patients in tertiary care teaching hospital. Materials and Methods: It was a prospective study in NICU of tertiary care hospital for duration of one year. Case record forms were used to collect the information regarding demographic details, maternal and delivery details, symptoms and co-morbidities in neonates with sepsis, final diagnosis and medications administered. Antimicrobials and other drugs use data such as selection of drug, dosage form and route of administration, duration were collected as per WHO guideline for drug utilization study (core indicators) and were analyzed using SPSS. Results and Discussion: Majority of the patient (42.85%) had low birth weight of less than 2.5 kg. Prematurity is a major cause of low birth weight (less than 2.5kg). 82.5% of patients admitted were...
Read More
Posted by admin on Nov 30, 2025 in |
P-glycoprotein (P-gp) is a membrane efflux transporter influencing drug absorption and disposition. Inhibiting P-gp may increase plasma levels of substrates like digoxin, heightening toxicity risk. This study aimed to classify 35 drugs based on their molecular docking-derived binding affinity to P-gp and their potential to inhibit digoxin transport, using estimated inhibition constants (Ki). Docking simulations were performed using AutoDockVina. Physicochemical parameters were gathered, and binding energies (ΔG) were converted to Ki values. Drugs were ranked using a Ki ratio relative to digoxin (Ki-drug/Ki-dgx). Four drugs (conivaptan, telmisartan, indinavir, and troglitazone) showed stronger affinity than digoxin (Ki < Ki-dgx), indicating high risk of interaction. Diltiazem displayed indirect interaction through aldosterone modulation. The classification revealed 12% strong, 56% moderate, and 32% weak inhibitors. This computational framework allows early screening of P-gp-mediated drug–drug interactions and can guide clinical decisions involving digoxin therapy in polypharmacy...
Read More
Posted by admin on Nov 30, 2025 in |
Mesalamine is a key therapeutic agent used in the treatment of inflammatory bowel diseases such as ulcerative colitis and Crohn’s disease. Ensuring its purity and controlling related impurities is critical for drug safety and efficacy. This study focuses on the development and validation of a robust reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous detection and quantification of three structurally related impurities Impurity G, Impurity K, and Impurity L in mesalamine pharmaceutical formulations. The chromatographic separation was achieved using an Agilent C18 column with an isocratic mobile phase composed of methanol and 0.1% acetic acid (52:48, v/v), at a flow rate of 0.85 mL/min, with detection at 229 nm. The method was validated in accordance with ICH Q2(R2) guidelines, covering critical parameters such as specificity, linearity (R² ≥ 0.999), precision (%RSD ≤ 0.65), accuracy (% recovery 94.8%–96%), robustness, limit of detection (0.20 µg/mL), and limit of quantification (0.62 µg/mL). The assay demonstrated high reliability for quality control applications and routine analysis of mesalamine products. Additionally, the method showed...
Read More
Posted by admin on Nov 30, 2025 in |
The present study aimed to develop and validate a simple, precise, and accurate UV spectrophotometric method for the estimation of atorvastatin in bulk and pharmaceutical dosage forms. The method exhibited excellent linearity over the concentration range of 20–120 µg/ml with a correlation coefficient (R²) of 0.9996 and a regression equation of y = 0.01x + 0.0048. Precision was confirmed through intra-day and inter-day studies, showing low %RSD values of 0.2598 and 0.2987, respectively. Accuracy, evaluated via recovery studies at 80%, 100%, and 120% concentration levels, yielded a mean recovery of 99.65% ± 1.25, demonstrating the method’s reliability. The method also proved sensitive with a Limit of Detection (LOD) of 0.19872 µg/ml and a Limit of Quantification (LOQ) of 0.652387 µg/ml. Specificity studies confirmed no interference from formulation excipients. Additional parameters, including molar absorptivity (2289.23) and Sandell’s sensitivity (0.125), further validated the method’s robustness. The results affirm that this UV spectrophotometric method meets ICH validation criteria and is suitable for routine quality control of...
Read More
