Posted by admin on Jun 30, 2026 in |
The objective of the current research work includes development and validation of novel, precise and accurate UV-visible spectrophotometric techniques for the analysis of Cilnidipine and Chlorthalidone without the need of prior separation by employing mathematical spectrophotometric manipulations, which utilize absorbance relationships to resolve overlapping spectral components. Two methods, namely Ratio Subtraction Coupled with Spectrum Subtraction (RS-SS) and Absorption Factor Method (AFM) were developed for the simultaneous estimation of Cilnidipine (CIL) and Chlorthalidone (CHLOR). In the RS-SS method, CIL and CHLOR were quantified at 365 nm and 275 nm, respectively, using a divisor concentration of 8 µg/mL of CIL. In the AFM method, CHLOR concentration was quantified directly by recording the absorbance at 275 nm. For the estimation of CIL, an absorption factor was calculated from the absorbance values at two selected wavelengths, 275 nm and 365 nm. These values were applied in the AFM equation to compute the absorbance attributable to CIL in the mixture. The developed methods were validated in accordance with ICH Q2 (R2) guidelines. Linearity was established over the concentration...
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Posted by admin on Jun 30, 2026 in |
Curcumin, the principal bioactive component of turmeric, is well-documented for its broad spectrum of pharmacological properties, including antioxidant and antibacterial activities. Nevertheless, its therapeutic use is restricted due to low solubility and limited bioavailability. In this study, eight novel curcumin derivatives (1A–8A) were synthesized using a microwave-assisted organic synthesis (MAOS) approach to enhance pharmacological efficacy. The synthetic strategy involved initial acetylation of curcumin followed by Biginelli-type cyclocondensation to incorporate heterocycles. All synthesized analogues were characterized using FT-IR, ¹H-NMR, and LC-MS. Antibacterial activity was assessed against Escherichia coli via broth dilution methods, and minimum inhibitory concentrations (MICs) were determined. Notably, Compounds 4A (9 micrograms per milliliter), 2A (10 micrograms per milliliter), and 8A (11 micrograms permilliter) displayed superior antibacterial activity compared to both curcumin (14 µg/mL) and the standard antibiotic ampicillin (16 µg/mL). These results indicate that strategic structural modifications to the curcumin scaffold, particularly through heterocyclic incorporation, can yield derivatives with significantly enhanced antibacterial potential. This work highlights the promise of curcumin analogues as potent antibacterial agents and supports further pharmacological...
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Posted by admin on Jun 30, 2026 in |
Introduction: Osteoporosis is a systemic skeletal disorder marked by reduced bone strength and increased fracture risk, often due to alterations in trabecular bone microarchitecture. Tobacco use both smoked and smokeless has emerged as a modifiable risk factor influencing bone health. Panoramic radiography (PR), a common dental imaging modality, offers potential for early detection of osteoporosis through analysis of fractal dimension (FD) and radiomorphometric indices (RMIs). Materials and Methods: This study conducted a comparative evaluation of mandibular bone quality using digital panoramic radiographs and dual-energy X-ray absorptiometry (DXA) scans in tobacco users and non-users aged 20–40 years. Fractal dimension and radiomorphometric parameters including MI, MCI, and PMI were analysed using Image J software. BMD values were obtained using DXA, and statistical analysis was performed to evaluate correlations. Results: Tobacco users demonstrated significantly lower FD values and altered radiomorphometric indices compared to controls, indicating compromised bone quality. A positive correlation was observed between FD/RMIs and DXA-derived BMD values, suggesting their diagnostic utility in early osteoporosis screening. Conclusion: Fractal geometry and radiomorphometric...
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Posted by admin on Jun 30, 2026 in |
Declining soil fertility has emerged as a critical global concern in the face of rapid population growth and increasing food demand. Among the major factors contributing to reduced soil productivity is heavy metal contamination, with lead (Pb) recognised as one of the most toxic pollutants affecting both soil health and crop yield. In this study, rhizobacteria were isolated from soils collected near battery factories and screened for their tolerance to Pb. A total of five Pb-tolerant isolates were obtained and evaluated for their maximum tolerance levels. Biochemical characterization and plant growth-promoting rhizobacteria (PGPR) trait analysis revealed that three isolates exhibited significant PGPR activities. These three promising strains were subsequently tested in pot experiments using okra (Abelmoschus esculentus) as a pot experiment. Inoculation with Pb-tolerant PGPR enhanced plant growth under lead stress, demonstrating their potential to mitigate heavy metal toxicity while simultaneously improving crop performance. This work highlights the dual role of Pb-tolerant rhizobacteria in restoring soil fertility and supporting sustainable agriculture in contaminated...
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Posted by admin on Jun 30, 2026 in |
Introduction: Hemoglobin E (HbE) disorders and glucose-6-phosphate dehydrogenase (G6PD) deficiency are two important red cell genetic abnormalities highly prevalent in malaria-endemic regions of Southeast Asia. The Karbi tribe, one of the major ethnic groups of Northeast India, is considered to have a significant burden of these disorders; however, data on their prevalence remains limited. This study aimed to determine the prevalence of Hb E disease, HbE trait, and G6PD deficiency among Karbi tribal individuals attending a tertiary care centre in Northeast India. Methods: A total of 600 Karbi tribal patients were enrolled in this cross-sectional study facility based study. Demographic details including age, gender, and sub-clan distribution were recorded. High performance liquid chromatography was done for Hemoglobin variant and G6PD by a semi-automated analyzer. Data were analyzed for prevalence patterns across age groups, gender, and sub-clans. Results: Hemoglobin E trait was found in 84 subjects (14%) and Hemoglobin E disease in 12 individuals (2%). G6PD deficiency was observed in 36 individuals (6.0%). Females showed a higher prevalence of HbE...
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