Posted by admin on Jun 30, 2025 in |
This comprehensive study investigates the in vivo effects of Tupichinol E, osimertinib, and their combination in a xenograft model of triple-negative breast cancer (TNBC), an aggressive and therapeutically challenging breast cancer subtype. By employing a multi-faceted approach, the study evaluates tumor growth inhibition, apoptosis, and proliferation through histopathological and immunohistochemical techniques, including hematoxylin and eosin (H&E) staining, TUNEL staining, and Ki67 immunohistochemistry. The results reveal significant therapeutic potential. Tumor growth was markedly suppressed in all treated groups, with monotherapy using Tupichinol E or Osimertinib reduces tumor volume by approximately 35% and 40%, respectively. Notably, combination therapy achieved a synergistic effect, maintaining tumor volumes at baseline levels and preventing progression over the two-week treatment period (p<0.001). Histological analyses demonstrated extensive necrosis and disruption of tumor architecture in the combination group, correlating with a 150% increase in apoptotic activity as indicated by TUNEL staining (p<0.001). Furthermore, Ki67 immunohistochemistry showed a substantial 70% reduction in proliferative activity in the combination group compared to controls (p< 0.001). These findings underscore the enhanced efficacy...
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Posted by admin on Jun 30, 2025 in |
Chromatography is a separation process that is achieved by distributing the substances to be separated between a moving phase and a stationary phase. HPLC is high resolution, high pressure and high-speed liquid chromatography. It has several resolving powers than open column liquid chromatography hence it is used for speedy resolution of complex mixture, separation and determination of species in a variety of organic, inorganic and biological materials. The developed HPLC method was validated for various parameters like accuracy, precision, specificity, LOD, LOQ, linearity, range and robustness as per ICH guidelines. The results obtained were well within the acceptance criteria for all the parameters. The proposed method was applied for simultaneous estimation of Tamsulosin and Dutasteride formulation. The HPLC system used was WATERS Alliance series System with Rheodyne injector 20 μL and the column Inertsil ODS 250 x 4.6 mm, 5 μm. The mobile phase comprised of Sodium phosphate buffer and Acetonitrile in the ratio of 30:70 v/v and flow rate of 1.0 ml/min with UV detection at 233 nm...
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Posted by admin on May 31, 2025 in |
Medicinal plants are of great importance to researchers in the field of pharmacology as most pharmaceutical industries depend on medicinal plant for their raw materials. Meyna laxiflora belongs to the family Rubaceae and is well known for its medicinal properties. The present study was carried out in continuation of our previous findings to determine the possible bioactive components present in the aqueous and methanolic extracts of Meyna laxiflora fruit pulp. The gas chromatography – Mass spectrometry was used for identification of the bioactive compounds in the extracts and the spectrums of unknown compounds were compared with the compounds stored in the National Institute of Standards and Technology Mass Spectral database (NIST). The GC-MS analysis revealed the presence of 37 peaks in aqueous extract among these 19 were found to be major compounds while that of methanolic extract showed 24 peaks of which 15 were found to be major compounds. Hence, the present study has generated baseline data for further characterization of fruit pulp extract of this...
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Posted by admin on May 31, 2025 in |
Momordica cymbalaria, an underexplored vegetable crop native to India, holds significant therapeutic potential due to its traditional antidiabetic use. This study explores its phylogenetic characterization, protein structure modelling, and phytochemical profiling, emphasizing its role in diabetes management. The 5.8S rRNA internal transcribed spacer sequence was retrieved from NCBI and analysed using BLAST, identifying 17 related species and insights into its evolutionary positioning. Phylogenetic analysis revealed close relationships with Momordi cafoetida and distant similarity to Momordica balsamina. Protein structure prediction using SWISS-MODEL and PyMOL identified key structural features and active sites. Three open reading frames (ORFs) were identified, potentially encoding proteins involved in diabetes-related pathways. Phytochemical analysis using Dr. Duke’s and IMPPAT databases highlighted bioactive compounds such as beta-sitosterol, ergosterol, and alpha-eleostearic acid (ESA), a potent antidiabetic compound. ESA modulates the adipose deposition pathway and improves glucose regulation by interacting with proteins like peroxisome proliferator-activated receptor gamma (PPARG) and ribosomal protein L19. In-silico analysis demonstrated ESA’s ability to enhance GLUT-4 and beta-glucokinase activity, facilitating glucose uptake and improving metabolic efficiency....
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Posted by admin on May 31, 2025 in |
The most widely used cationic dye, methyl violet (MV), has significant carcinogenic potential. The textile, cosmetic, printing, pharmaceutical, and food processing sectors all make extensive use of artificial dyes. They are typically referred to as xenobiotics because of their strong resistance to biodegradation. Microbes are beneficial because of their rapid growth and ability to break down and mineralise dyes. Methyl violet-degrading Enterobacter sp. was isolated from the Ganges River. It degrades up to 180 ppm of methyl violet in 24 hours when shaken at 25°C – 45ºC and pH 1 -12; optimum degradation occurs at 40 ppm in 24 hours at pH 7.0. The effects of temperature, initial pH, and dye concentration were investigated. The capacity of bacteria to degrade was demonstrated by experimental results. The deterioration of methyl violet was validated by UV-Vis spectroscopy, SEM, and FTIR examination of samples both before and after dye breakdown in culture...
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