Posted by admin on Feb 28, 2022 in |
The present study aims at analyzing the efficiency of allicin, agarlic derived phytochemical or neutraceutical in elevating the activities of various enzymic antioxidants on its treatment to cultured breast cancer cell lines. In the experimental work, cultured breast cancer cell lines were treated with different doses of allicin, and through standardized protocols, the cell lines were assayed for the activities of enzymic antioxidants such as Peroxidase, Superoxide dismutase, Catalase and Glutathione S Transferase as compared to untreated control. It was found that allicin treatment significantly increased the activities of the said enzymic antioxidants as compared to control. This study demonstrates the antioxidant potential of allicin in cancer cells, showing decreased antioxidant activity as a characteristic feature of cancer cells, leading to severe oxidation-induced damage. The study also proposes future prospects in the areas of cancer research and therapy to cobble for the designing of targeted plant-based therapies that are easily available and are cost effective in comparison to synthetic therapeutic chemicals and that too without side...
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Posted by admin on Feb 28, 2022 in |
There is an urgent need to replace the injection currently used for low molecular weight heparin (LMWH) multi-dose therapy with a non-invasive delivery device. In this study, unsaturated polyester resin and peroxide were used to fabricate microneedle mould by using 3M microneedle. Dissolving microneedle arrays transdermal patch was prepared by using 20 % w/v polymers of PVA and PVP (polyvinyl alcohol and polyvinyl pyrrolidone) and active drug 20 mg (IU) low molecular weight heparin (LMWH). The physicochemical compatibility of the drug and the polymers was studied by infrared spectroscopy suggested the absence of any incompatibility. Formulated transdermal films were physically evaluated with regard to weight variation, drug content, folding endurance, percentage of moisture content and water uptake. All prepared formulations indicated good physical stability. In-vitro permeation studies of formulations were performed by using Franz diffusion cells. The formulation prepared with PVP: PVA 1:2 showed best ex-vivo skin permeation through rat skin (Wistar rat) as compared to all other formulations. The release profile of the optimized formulation F2 (R2 =...
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Posted by admin on Feb 28, 2022 in |
Polygonum glabrum, commonly called dense flower knotweed, is a perennial plant found in most parts of India. The leaves of plants Polygonum glabrum Willd. Belonging to the family, Polygonaceae are a rich source of flavonoids and tannins but not yet scientifically investigated for antioxidant activity. The present study aimed to offer a novel scientific background to the leaves of Polygonum glabrum by evaluating it for antioxidant activity using in-vitro and in-vivo methods. The ethyl acetate and ethanolic extracts of Polygonum glabrum Willd. leaves showed significant antioxidant activity by using in-vitro models, which may be due to the presence of phytoconstituents such as polyphenol, tannins and flavonoids. Based on the in-vitro results, potent extracts were further evaluated for antioxidant studies by using in-vivo models. Acute toxicity studies were carried out as per OECD guidelines, and the doses were fixed at 200 and 400 mg/kg b.w. The ethyl acetate and ethanolic extracts at the dose of 400 mg/kg showed the degree of protection by measuring antioxidant enzymes CAT, SOD, non-enzymatic antioxidant...
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Posted by admin on Feb 28, 2022 in |
Protein-protein, as well as protein-substrate interactions, play an important role in regulating specific biological functions like signal transduction, apoptosis, gene regulation, and immune response. These play a major role in drug discovery applications and can be used as molecular targets to stop disease development at the molecular level. Thus, understanding the biological mechanism of protein is an integral part of any computational study. Moreover, these interactions can be manipulated through protein engineering to address industrial applications. As part of the protein engineering strategy, the first step is the determination of hotspots. Hotspots are defined as positions in the amino acid sequence that can be targeted for mutagenesis to improve the catalytic activity or stability of an enzyme. The commonly-used method for determining hotspot residues is computational alanine scanning (CAS) mutagenesis experiments, which are computationally expensive and time-consuming. In the current study, we aim to develop a protocol to predict hotspots using a computationally less costly method. We have used two published datasets to cross-validate our method. Both the datasets...
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Posted by admin on Feb 28, 2022 in |
Objective: The Calotropis procera (Ait.) R.Br. (Ascelpidaeaceae) has been used traditionally as anthelmintic, depurative, diaphoretic, emetic, expectorant, febrifuge, and purgative, but no scientific reports are available in the regulation of asthmatic condition. In this present study, Calotropis procera (Ait.) R.Br. was selected to investigate scientifically to establish scientific data for its traditional claim. Materials and Methods: The anti-asthmatic activity of MECP (Methanolic extract of roots of Calotropis procera) and AECP (Aqueous extract of roots of Calotropis procera) was studied by using in-vitro and in-vivo models. The reference drug used was chlorpheniramine (CPM) and Dexamethasone. The in-vitro models included isolated goat tracheal chain and guinea pig ileum preparation. Further, the in-vivo included histamine-induced bronchospasm, milk-induced leukocytosis, passive paw anaphylaxis model, and haloperidol-induced catalepsy. Results: In the in-vitro and in-vivo models, the oral dose of both MECP and AECP showed significant results as compared to control. Among both extracts, MECP showed better activity in the prevention of asthma. Conclusion: Based on phytochemical analysis of the root’s extracts of Calotropis procera (Ait.)...
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