Posted by admin on Dec 31, 2021 in |
Urena lobata is used by traditional practitioners for treating external wounds. A preclinical study was conducted to evaluate the efficacy of the ointment formulations containing ethanolic extract of Urena lobata (EO) (5%) and nanosized extract (NO) of U. lobata (5%) for their wound healing activity by assessing wound contraction, tensile strength, superoxide dismutase, catalase, reduced glutathione, myeloperoxidase, hydroxyproline, lipid peroxidation and his to pathological studies in experimentally-induced open wounds in Swiss albino rats. Quercetin, an active principle of the formulation, was estimated qualitatively and quantitatively through HPLC studies. Topical application of NO and EO formulations promoted faster reepithelialization, wound contraction and maximized tensile strength, increased SOD, catalase activities and GSH, hydroxyproline levels with lower myeloperoxidase and lipid peroxidation in comparison with control. Histopathological studies revealed lesser inflammatory cell proliferation, degeneration, with marked fibroblast proliferation and collagen maturation than the control group. Results indicate that both NO and EO formulations showed benefits in the management of wounds when compared to the standard povidone...
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Posted by admin on Dec 31, 2021 in |
Fostemsavir was quantified using a simple and reliable HPLC technique developed and validated. PhenomenexC18 column was used to achieve chromatographic segregation (150 x 4.6 mm, 5m). The mobile phase was a 55:45 mixture of 0.01N disodium orthophosphate and acetonitrile pumped through the column at 1.0ml/min. The temperature was maintained at 30 °C and the buffer used was 0.01N sodium dihydrogen phosphate. The wavelength of the ideal choice was 278.0nm. Fostemsavir’s retention time was discovered to be 2.471 minutes. Fostemsavir’s percent RSD was found to be 0.7 percent. For Fostemsavir, the percentage recovery was 100.07 percent. Fostemsavir’s regression equation yielded LOD and LOQ values of 0.18 and 0.54, respectively. Fostemsavir’s regression equation is y = 26381x + 8398.8. Since run time and retention time were minimized, the system developed was simple and cost-effective, and it could be used on a daily basis for quality...
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Posted by admin on Dec 31, 2021 in |
To study the hepatoprotective activity of ethanolic leaves extracts of Abroma augusta against non-alcoholic fatty liver disease to validate ethnobotanical claim regarding the plant used in the above mentioned disease. Materials and Methods: The hepatoprotective potential of Abroma augusta leaves extract (AALE) (250 and 500 mg/kg/ body weight) was studied on Methionine and Choline deficient diet (MCD diet), High Fat Diet (HFD), Cholesterol and Cholate diet (CCD) and streptozotocin + HFD induced non-alcoholic fatty liver disease. In the last treatment, blood was collected from direct cardiac puncture and analyzed for various parameters like alanine aminotransferase (ALT), aspartate transaminase (AST), triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), free fatty acid (FFA) and malondialdehyde (MDA). Results: The present study showed for the first time that AALE reduced level of ALT, AST, TG, TC LDL, FFA, MDA and possessed hepatoprotective activity as evidenced by its significant inhibition in the formation of hepatoxicity induced by diet and chemical agents with maximum curation (500mg/kg/b.w.) against non-alcoholic fatty liver disease. It...
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Posted by admin on Dec 31, 2021 in |
Objective: The research work aims to formulate and evaluate the Gastro retentive mucoadhesive film of Ritonavir using polymers like HPMC K15M, Polyvinyl alcohol, and PEG 400 as a plasticizer by solvent casting method for treatment of AIDS. Method: Prepared gastro retentive mucoadhesive films were evaluated for various parameters such as in-vitro unfolding behavior of film, Folding endurance, Percent swelling, Drug content, and in-vitro drug release studies. Results: The release rate of the gastro retentive mucoadhesive films of Ritonavir was found to obey Korsmeyer-Peppas kinetics. After analysis of different evaluation parameters and drug release kinetics. Conclusion: Formulation code F4 was selected as a promising formulation for delivery of Ritonavir as a muco-adhesive Gastro retentive film with required in-vitro parameters 99.08% drug release at 12th...
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Posted by admin on Dec 31, 2021 in |
The application of mathematically based prognostic software is a rational and promising approach to determining disease prognoses. In our research, we identified indicators as prognostic criteria for the development of atopic dermatitis (AD). Our research involved 260 healthy children and 268 newborns with AD. To select the risk factors for predicting the formation of AD, initially, variation and discriminant analyses were used to identify 50 indicators (out of 109 clinical laboratory indicators) that differed statistically. Then, 28 indicators with a high impact on the final result were selected via disbursement analysis; subsequently, 19 indicators based on specificity and sensitivity were fixed. In the next step, via correlation analysis, we focused on 15 antenatal, intranatal, neonatal, and laboratory risk factors that did not correlate with each other. Based on these indicators, software for the prediction of atopic dermatitis was developed. The software-based on MS EXCEL was developed with the application of theories of probability and “fuzzy logic”, and the Sp and Sn of the test were evaluated accordingly at 78.5±2.5%...
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